Fig. 1. Adenine modulates Bax and Bcl-2 protein levels. (A) B16-F10 cells were exposed to various doses of adenine for 24 h in complete media. Cell lysates were used for Western blotting to detect indicated proteins. (B) Densitometry analysis of Bax and Bcl-2 done using ImageJ software and ratio of Bax and Bcl-2 was calculated. Data presented after normalizing the ratio with untreated control group. The data shown are representative of three independent experiments. Significant difference against control group is indicated as *P < 0.05.
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