Fig. 1. Quantitative PCR TaqMan probe assay for GAPDH gene with concentration from 1 × 108 copies/μL to 1 × 101 copies/μL by 10-fold serial dilution. (A) Amplification curve (X-axis: Cycles, Y-axis: RFU, Relative Fluorescence Unit) and (B) Standard curve (X-axis: Log Starting Quantity, Y-axis: Cq, quantification cycle) of the target GAPDH gene plasmids.
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