Fig. 2. Amplification plot of qPCR for the target synthesized GAPDH gene and negative sample including nine bacterial and five fungal reference strains. Amplification curves (X-axis: cycle, Y-axis: RFU, relative fluorescence unit) of the GAPDH plasmid gene as positive control and nine bacterial and five fungal reference strains as negative control and non-template control (NTC). Nine bacterial reference strains included S. epidermidis, S. aureus, E. faecalis, S. dysgalactiae, S. pyogenes, E. coli, A. baumannii, P. aeruginosa and E. cloacae. Five fungal reference strains included C. parapsilosis, C. tropicalis, C. glabrata, C. albicans, A. fumigatus.
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