Biomedical Science Letters

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Table. 3.

Mean Ct values for qPCR carried out on serial dilutions of plasmid DNA of the GAPDH gene. SD is standard deviation

No. GAPDH plasmid
Plasmid DNA content Mean Ct value SD Log value Copies per reaction (copies/μL)
1 1.782 ng 8.99 0.10 8.24 1.74×108
2 178.2 pg 14.59 0.08 6.93 8.58×106
3 17.82 pg 19.06 0.02 5.89 7.78×105
4 1.782 pg 23.88 0.15 4.77 5.83×104
5 178.2 fg 27.23 0.06 3.98 9.66×103
6 17.82 fg 31.16 0.03 3.07 1.17×103
7 1.782 fg 34.97 0.11 2.18 1.51×102
8 178.2 ag 40.00 0.00 1.00 1.01×101
Biomed Sci Letters 2023;29:220-30 https://doi.org/10.15616/BSL.2023.29.4.220
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