Biomedical Science Letters

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Table. 4.

Result of qPCR for GAPDH plasmid gene and reference strains in this study

Gene and reference strains Control type Real-Time PCR using design of primers and probe in This Study
Mean Ct value Standard deviation
GAPDH gene The synthesized GAPDH gene (This study) Internal control 3.94 0.45
Bacterial strains ATCC 35989 Staphylococcus epidermidis Negative control N/A*
ATCC 29213 Staphylococcus aureus Negative control N/A*
KCTC 3511 Enterococcus faecalis Negative control N/A*
KCTC 3098 Streptococcus dysgalactiae subsp. equismilis Negative control N/A*
ATCC 19615 Streptococcus pyogenes Negative control N/A*
ATCC 35150 Escherichia coli O157: H7 Negative control N/A*
KCTC 23254 Acinetobacter baumannii Negative control N/A*
KCTC 22063 Pseudomonas aeruginosa Negative control N/A*
ATCC 2361 Enterobacter cloacae Negative control N/A*
Fungal strains KCTC 7653 Candida parapsilosis Negative control N/A*
KCTC 7212 Candida tropicalis Negative control N/A*
KCTC 7219 Candida glabrata Negative control N/A*
ATCC 10231 Candida albicans Negative control N/A*
KCTC 6145 Aspergillus fumigatus Negative control N/A*

*Not detection

†The following abbreviations are used: KCTC, Korean Collection for Type Cultures, Taejon, Korea; ATCC, American Type Culture Collection, Rockville, MD, USA

Biomed Sci Letters 2023;29:220-30 https://doi.org/10.15616/BSL.2023.29.4.220
© 2023 Biomed Sci Letters