Biomedical Science Letters

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Fig. 1. Gel retardation assay for mRNA encapsulation efficiency of LNPs at various N/P ratios.
The encapsulation efficiency of various LNP formulations was determined using gel retardation assay. For each LNP, encapsulation was performed using 100 ng of mRNA at various N/P ratios for 30 min at room temperature. This was followed by gel lag on a 1.2% agarose gel at 100 V for 20 min. The mRNA bands were then stained with SYBR Green II diluted 1:10,000 in TBE buffer and measured using UV illumination.
Biomed Sci Letters 2023;29:231-41
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