Biomedical Science Letters

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Fig. 3. Optimization of N/P ratio for in vitro transfection by luciferase assay.
(A and B) A549 and BEAS-2B cells were transfected with Fluc mRNA encapsulated in each LNP to determine the optimal N/P ratio. Encapsulation was carried out at room temperature for 30 min at each N/P ratio. Following transfection, cells were incubated for 3 h and then stabilized at 37℃ with 5% CO2 for an additional 21 h. After this incubation period, cell lysates were obtained by treating with 1X CCL buffer at room temperature for 2 h, collected in microtubes, and centrifuged at 12,000 rpm for 1 min to obtain the supernatant. Subsequently, the supernatant was measured with luciferase assay reagent by luminometer (n = 3; mean ± SD, nsP >0.05, *P < 0.05, **P < 0.01, and ***P < 0.001; Student's t-test, two-tailed).
Biomed Sci Letters 2023;29:231-41
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