Fig. 4. Cytotoxicity assessment of LNP/mRNA complexes.
(A and B) The cytotoxicity of LNP/mRNA complexes was verified by encapsulating LNPs with mRNA at various N/P ratios, followed by a 24 h incubation at 37℃ with 5% CO2. A positive control was transfected using lipofectamine 3000. Subsequently, MTT reagent was diluted to 10% in serum-free media and incubated at 37℃ with 5% CO2 for 2 h 30 min. The resulting formazan particles were dissolved by treatment with DMSO for 30 min. The optical density (OD) value was measured using a microplate reader at 570 nm.
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