Fig. 6. Determination of accumulation time of nebulized Rho-LNP/mRNAs in BEAS-2B cells.
To determine the accumulation time of LNPs within cells, Rho-LNPs were synthesized by replacing DOPE with Rho-DOPE. The resulting Rho-LNP was nebulized using a nebulizer and subsequently applied to BEAS-2B cells. After incubation for various durations, the cells were stabilized with 10% medium, subjected to trypsin treatment, harvested, and the accumulated rhodamine within the cells was quantified using a flow cytometer.
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