Fig. 7. Luciferase and cytokine expression in vivo after LNP/mRNA administration.
Mice were administered with LNP/mRNAs via i.v. injection and inhalation. After 6 h, the mice were euthanized, and the lungs, livers and BAL fluid were promptly harvested, then immediately frozen at -80℃. (A) Tissue lysates were prepared by homogenizing the lungs and liver in 1X CCL buffer. The resulting supernatant was collected through centrifugation, and a luciferase assay was conducted by luminometer. (B) Expression levels of IL-1β and IL-6 were assessed using the ELISA method in cells from the BAL fluid. The procedure was conducted as instructed in the manual, and measurements were taken using a microplate reader at 450 nm. (n = 3; mean ± SD, *P < 0.05, ***P < 0.001 as compared with control; Student's t-test, two-tailed).
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