Biomedical Science Letters

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Fig. 8. In vivo Experiments with Selected LNPs.
Based on the in vitro experiment results, LNP1, LNP2-2, and LNP3-2 were chosen for further evaluation in animal experiments. CleanCap Fluc mRNA was prepared at a concentration of 0.5 mg/kg in mice and encapsulated with each LNP at an N/P ratio of 6:1. The formulations were then administered via intravenous injection or nebulization. After 6 h, the mice were euthanized, and bronchoalveolar lavage (BAL) fluid was collected using 1 mL of PBS. The lungs and liver were harvested and immediately frozen at -80℃. Subsequently, tissue samples and BAL fluid were subjected to Luciferase assay to quantify mRNA expression and ELISA to assess toxicity.
Biomed Sci Letters 2023;29:231-41
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