Biomedical Science Letters

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Table. 4.

Viability and overgrowth tests after storage at refrigerated and room temperature (The solid type with charcoal)

Organism Type Recovery at concentration of a
105 CFU/mL at (h) 104 CFU/mL at (h) 103 CFU/mL at (h)
5 min 24 hr, 48 hr 5 min 24 hr, 48 hr 5 min 24 hr, 48 hr
RT 4℃ RT 4℃ RT 4℃
Aerobic, facultative anaerobic bacteria
P. aeruginosa A 907 819 160 157 14 13
B 820 764 152 150 13 12
S. pyogenes A 327 252 30 24 3 3
B 350 273 44 26 4 2
S. pneumoniae A 809 837 104 112 9 9
B 937 854 122 115 10 9
H. influenzae A 1525 1400 221 184 18 18
B 1413 1418 180 196 20 19
Anaerobic bacteria
B. fragilis A 1545 1450 329 294 32 29
B 1522 1360 322 296 31 28
P. anaerobius A 1530 1521 247 240 26 24
B 1490 1571 243 254 26 26
F. nucleatum A 1282 1135 251 211 23 21
B 1347 1251 286 234 25 22
C. acnes A 1554 1447 327 309 31 31
B 1508 1461 301 317 35 34
P. melaninogenica A 1557 1677 253 252 23 22
B 1353 1607 228 248 22 22
Fastidious bacteria
N. gonorrhoeae A 1062 974 218 187 27 29
B 993 931 208 177 29 25

aAverage of triplicate tests in CFU/100 μL. RT, room (ambient) temperature

A and B the date of manufacture and expiration date are different

Biomed Sci Letters 2023;29:249-55 https://doi.org/10.15616/BSL.2023.29.4.249
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