Primer sets for RT-PCR
| Gene | Primer sequence (5' - 3') | Annealing temperature (℃) | PCR product size (bp) |
|---|---|---|---|
| MMP-2 | F : GCGACAAGAAGTATCGCTTC | 58 | 390 |
| R : TGCCAAGGTCAATGTCAGGA | |||
| MMP-9 | F : CCATTTCGACGATGACGAGTT | 58 | 530 |
| R : CTTGTCGCTGTCAAAGTTCGAG | |||
| GAPDH | F : GAAGGTGAAGGTCGGAGT | 58 | 226 |
| R : GAAGATGGTGATGGGATTTC |
