Fig. 12. This figure visualizes the key findings regarding the final selection of conditions for the antibody-quantum dot conjugate manufacturing. It visually demonstrates that an effective binding between antibodies and quantum dots can be achieved when the EDC/sulfo-NHS molar ratio is set at 1:2. The figure also presents sensitivity comparison experiments between conditions using pH 6.0 MES buffer for the EDC/NHS reaction and conditions using pH 7.4 PB buffer. The experimental results indicate that the conditions with the reaction carried out in pH 6.0 MES buffer exhibited over two times higher sensitivity than the conventional pH 7.4 PB buffer. It signifies that the final conditions involved setting the EDC/sulfo-NHS molar ratio to 1:2 and using pH 6.0 MES buffer for conjugate manufacturing. These conditions allowed for effective binding between antibodies and quantum dots while achieving a high level of sensitivity simultaneously.
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