Biomedical Science Letters

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Fig. 3. iNOS mediates UVB-induced increased expression of pro-inflammatory cytokines in Jurkat T cells exposed to TGs.
Jurkat T cells were treated with TG (1.0 mg/mL) and irradiated with UVB (100 J/m2) in the absence or presence of the iNOS inhibitor 1,400 W (0, 10, or 20 µM) for 24 h. (A) The mRNA level of TNF-α was analyzed using RT-PCR. (B) The mRNA level of IL-1β was analyzed using RT-PCR. (C) The mRNA level of INF-γ was analyzed using RT-PCR. GAPDH was used as internal control. Data are expressed as the mean ± SD and are presented as the expression levels of mRNA relative to GAPDH mRNA. The expression level of mRNA relative to GAPDH in Jurkat T cells without treatment TG and UVB was set as 1.0. Data are from three independent experiments. P-values were determined with Student's t-test. *P < 0.05, **P < 0.01, ***P < 0.001.
Biomed Sci Letters 2023;29:376-81 https://doi.org/10.15616/BSL.2023.29.4.376
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