Fig. 5. Effect of the P. tectorius extract on the Notch signaling pathway in AGS cells.
(A-C) qPCR analysis showing the relative gene expression levels of HES1 (A), HES5 (B), and NOTCH1 (C) in AGS cells treated with 600 μg/mL of the P. tectorius extract compared to those in the control. Data are represented as the mean ± SEM of three independent experiments. *P < 0.05 compared to the control group. (D) Western blotting analysis showing the protein levels of HES1, HES5, and NOTCH1 in AGS cells treated with the P. tectorius extract. β-actin was used as a loading control. The results indicate the significant reduction in the protein levels of these markers in the treated group.
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