Fig. 2. Arctigenin induces apoptosis in SK-BR-3 cells. (A) SK-BR-3 cells were treated with an indicated dose of arctigenin for 24 hours, then stained with annexin V-FITC and PI, and subjected to flow cytometry. Stained cells were analyzed and illustrated on the quadrant by CellQuestPro software. (B) The percentage of apoptotic or live cells from flow cytometry results was analyzed and illustrated as a graph. (C) The cell lysates were subjected to Western blot to evaluate the levels of PARP, cleaved PARP, caspase 3, cleaved caspase 3, caspase 8, cleaved caspase 8, caspase 9, cleaved caspase 9, Survivin, and XIAP. GAPDH was used as an internal control. (D) Densities of the Western blot bands were illustrated as a graph and the results from triplicate experiments were analyzed by Student’s t-test (*P < 0.05, **P < 0.01, and ***P < 0.001).
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