Fig. 3. DI extract suppress the anti-apoptotic effect of Der p 38 on neutrophils. (A) Neutrophils were isolated and incubated for 24 hours in the absence or presence of Der p 38 (10 μg/mL) or/and DI extract (50 μg/mL). Apoptotic cells were evaluated using annexin-PI staining. Control was set to be 100% (n = 3). (B, D) Expressions of cleaved caspase 9, caspase 3, BCL-2, and BAX were detected by western blotting. (C, E) Densitometric data from B and D were presented relative to the negative control set at 1. (F) Ratio of BCL-2/BAX was calculated by densitometric analysis. DI, Duchesnea indica; Der p, Dermatophagoides pteronissinus; BCL-2, B-cell leukemia/lymphoma 2 protein; BAX, BCL-2-associated protein X; ERK, extracellular signal-regulated kinase; PI, propidium iodide; SD, standard deviation. The data are presented as the mean ± SD. *P < 0.05 and **P < 0.01 indicate a significant difference between the control and Der p 38-treated groups. #P < 0.05 and ##P < 0.01 indicate a significant difference between the Der p 38 and Der p 38 + DI-treated groups.
© 2024 Biomed Sci Letters