Biomedical Science Letters

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Fig. 4. The supernatant from BEAS-2B cells treated with Der p 38 and DI extract suppresses the anti-apoptotic effect of Der p 38 on neutrophils. BEAS-2B cells were treated with Der p 38 (10 μg/mL) or/and DI extract (50 μg/mL) for 48 hours. The supernatants were collected and used for treatment of neutrophils. (A) The supernatant-treated neutrophils were incubated for 24 hours. Apoptotic cells were evaluated using annexin-PI staining. Control was set to be 100%. (B, D) Expressions of cleaved caspase 9, caspase 3, BCL-2, and BAX were detected by western blotting. (C, E). Densitometric data from B and D were presented relative to the negative control set at 1. (F) Ratio of BCL-2/BAX was calculated by densitometric analysis. Der p, Dermatophagoides pteronissinus; DI, Duchesnea indica; BCL-2, B-cell leukemia/lymphoma 2 protein; BAX, BCL-2-associated protein X; ERK, extracellular signal-regulated kinase; PI, propidium iodide; SD, standard deviation. The data are presented as the mean ± SD. *P < 0.05 and **P < 0.01 indicates a significant difference between the control and Der p 38-treated groups. ##P < 0.01 indicates a significant difference between the Der p 38 and Der p 38 + DI-treated groups.
Biomed Sci Letters 2024;30:219-27 https://doi.org/10.15616/BSL.2024.30.4.219
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