Biomedical Science Letters

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Fig. 4. Effect of Angelica gigas Nakai (AG) on (A) IκB‐α degradation and (B) nuclear factor-κB (NF-κB) activation in activated human mast cells-1. Cells (7 × 106 cells/well) were incubated with various concentration of AG (0.1 and 1 mg/mL) for 1 hour and then stimulated by PMA plus 1 mg/mL of calcium Ionophore A23187 (PMACI) for 2 hours. Cells were harvest and cytosol and nuclear proteins were isolated. Cytosolic extracts for IκB‐α expression and nuclear extracts for NF-κB were evaluated by Western blot analysis. Dexamethasone (Dex, 100 nM) was used as positive control. Results are shown the mean ± standard deviation of experiments. #P < 0.05, significantly different from control; *P < 0.05, significantly different from PMACI-treatment.
Biomed Sci Letters 2024;30:238-47 https://doi.org/10.15616/BSL.2024.30.4.238
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