Biomedical Science Letters

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Fig. 2. Intracellular cytokine staining analysis in latent tuberculosis infection (LTBI) and healthy control (HC) groups. (A) Gating strategy for identifying polyfunctional CD4+ T cells. For each sample, singlets are selected, followed by excluding CD14+ and CD19+ cells. Lymphoid and CD3+CD4+ T cells are gated sequentially, and cytokine gates were applied. (B) Data show single-cytokine expression levels, including interferon-gamma (IFN-γ), interleukin-2 (IL-2), and tumor necrosis factor-alpha (TNF-α), for LTBI individuals and HCs. (C) Polyfunctionality of CD4+ T cells is compared between LTBI individuals (red plots) and HCs (blue plots). *P < 0.05, **P < 0.001, ***P < 0.0001, ****P < 0.00001.
Biomed Sci Letters 2024;30:335-40 https://doi.org/10.15616/BSL.2024.30.4.335
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