Biomedical Science Letters

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Fig. 2.

Pristimerin induces apoptosis in human colon cancer cells. (A) HCT116 and SW480 cells were treated with indicated concentration of pristimerin for 24 h and apoptosis was evaluated using Annexin V Assay Kit. The lower right quadrant (Annexin V+/ 7-AAD-) represents early apoptosis, and the upper right quadrant (Annexin V+/PI+) represents late apoptotic cells. Percentages of early and late apoptotic cells in (B) HCT116 and (C) SW480 cells are shown. (D) HCT116 and (E) SW480 cells were treated with indicated concentration of pristimerin for 24 h. Equal amounts of cell lysates were subjected to Western blot analysis for indicated proteins. β-actin was served as an equal loading control. Relative protein expression is presented in the bar graphs below. The results shown are the mean ± S.E.M. of four independent experiments. Statistical significance is based on the difference when compared with 0 nM-treated cells, *P < 0.05, **P < 0.01, ***P < 0.001.

Biomed Sci Letters 2018;24:15-22 https://doi.org/10.15616/BSL.2018.24.1.15
© 2018 Biomed Sci Letters