Biomedical Science Letters

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Fig. 3.

The dissipation of mitochondrial membrane potential (MMP) of colon cancer cells upon treatment with pristimerin. Western blot analysis showing BAX, Bcl-2, Bcl-xL and tBid protein levels in (A) HCT116 and (B) SW480 cells with the same treatments as in Fig. 2. Equal amounts of cell lysates were were subjected to Western blot analysis for indicated proteins. β-actin was served as an equal loading control. Relative protein expression is presented in the bar graphs below. HCT116 (C) and SW480 cells (D) treated with indicated concentrations of pristimerin for 24 h were stained with JC-1 and the average ratio of red/green fluorescence intensity was analyzed by flow cytometry. The data shown are representative of three independent experiments. Relative protein expression is presented in the bar graphs below. The results shown are the mean ± S.E.M. of four independent experiments. Statistical significance is based on the difference when compared with 0 nM-treated cells, **P < 0.01, ***P < 0.001.

Biomed Sci Letters 2018;24:15-22 https://doi.org/10.15616/BSL.2018.24.1.15
© 2018 Biomed Sci Letters