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Comparison of O-serogroups, Virulence Factors and Phylogenetic Groups of Uropathogenic Escherichia coli Isolated from Patients with Urinary Tract Infections between 2 Time Periods of 1989 and 2010-2014 at Gangwon Province in Korea
Biomed Sci Letters 2022;28:127-136
Published online June 30, 2022;  https://doi.org/10.15616/BSL.2022.28.2.127
© 2022 The Korean Society For Biomedical Laboratory Sciences.

Min Park* and Seong-Mi Kim†,*

Department of Biomedical Laboratory Science, Masan University, Changwon 51217, Korea
Correspondence to: Seong-Mi Kim. Department of Biomedical Laboratory Science, Masan University, 2640, Hamma-daero, Naeseo-eup, Masanhoewon-gu, Changwon-si, Gyeongsangnam-do 51217, Korea.
Tel: +82-55-230-1272, Fax: +82-55-230-1442, e-mail: smkim@masan.ac.kr
*Professor.
Received June 17, 2022; Revised June 29, 2022; Accepted June 30, 2022.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
 Abstract
Uropathogenic Escherichia coli (UPEC) is main causative agent of urinary tract infections. They are classified based on various types of O antigen. UPEC strains commonly possess many genes encoding virulece-associated factors. E. coli strains are generally divided into four main phylogenetic groups. The virulence factor (VF) profiles of UPEC are related with their O-serogroups in each strains. A total of 681 strains of UPEC clinical isolates were collected from Korean healthcare facility (1989: 123 strains and 2010-2014: 558 strains). The UPEC clinical isolates were analyzed by polymerase chain reaction (PCR) methods. A total of 14 O-serotypes (O1, O2, O4, O6, O7, O8, O15, O16, O18, O21, O22, O25, O75 and O83), 6 virulence factors (papC, fimG/H, sfaD/E, hly1, cnf1 and usp) and phylogenetic groups were identified. The most prevalent O-serogroups were O6 (11.1%) in 1989 UPEC strains and O25 (21.0%) in 2010-2014 UPEC strains. The identified VFs, phylogenetic groups in 1989 UPEC strains and 2010-2014 UPEC strains were fimG/H and B2 group. In this study, O6 serotype was revealed the close relationships with VFs. Also, the distribution of prevalence O-serogroups of UPEC has been changed from O6 to O25 and virulence of UPEC strains was increased during past twenty-one years.
Keywords : Uropathogenic Escherichia coli, O-serogroup, Virulence factor, Phylogenetic group
꽌 濡

Escherichia coli뒗 꽭洹좎꽦 媛먯뿼吏덊솚쓣 媛옣 留롮씠 쑀諛쒗븯뒗 꽭洹 以 븯굹씠떎(DebRoy et al., 2011). E. coli뒗 洹몃엺쓬꽦 留됰洹좎쑝濡 옣궡꽭洹좉낵뿉 냽븯硫, 젙긽꽭洹좊Т由щ줈 옣궡뿉 議댁옱븷 븣뒗 媛먯뿼利앹쓣 쑀諛쒗븯吏 븡吏留 옣궡媛 븘땶 씤泥댁쓽 떎瑜 遺쐞뿉 삤뿼릺뿀쓣 寃쎌슦 湲고쉶媛먯뿼쓣 쑀諛쒗븯뒗 寃껋쑝濡 븣젮졇 엳떎(Smith et al., 2007). 옣愿 씠쇅쓽 媛먯뿼利앷낵 愿젴맂 옣洹 以 슂濡쒕퀝썝꽦 옣洹(uropathogenic Escherichia coli, UPEC) 臾댁쬆긽꽭洹좊눊, 湲됱꽦 諛⑷킅뿼 諛 湲됱꽦떊슦떊뿼 벑쓽 슂濡쒓컧뿼쓣 쑀諛쒗븯뒗 寃껋쑝濡 븣젮吏 옣쇅蹂묒썝꽦 옣洹(extra-intestinal pathogeic E. coli) 以 븯굹씠떎(Fhin, 2003). 슂濡쒓컧뿼(urinary tract infection, UTI)쓣 쑀諛쒗븯뒗 寃껋쑝濡 븣젮吏 꽭洹좎뿉뒗 E. coli, Proteus spp., Enterobacter aerogenes 媛숈 옣궡꽭洹좉낵뿉 냽븯뒗 꽭洹, Pseudomonas aeruginosa, Enterococcus faecalis, Staphylococcus aureus, Staphylococcus saprophyticus 벑씠 엳떎(Lee and Kim, 1996; Fhin, 2003). 씠 以 슂濡쒓컧뿼쓣 媛옣 쓷븯寃 쑀諛쒗븯뒗 寃껋 E. coli씤 寃껋쑝濡 븣젮졇 엳떎(Struelens et al., 2004). 삉븳 옣洹좎 O (lipopolysaccharide), H (flagella) 洹몃━怨 K (capsular antigens) antigen뿉 뵲씪 삁泥삎쑝濡 遺꾨쪟븷 닔 엳뒗뜲, 쁽옱源뚯 蹂닿퀬맂 O 삁泥삎 170뿬醫 씠긽쑝濡 븣젮졇 엳떎(Liu et al., 2008). 옣洹좎 O 삁泥삎뿉 뵲씪 蹂묒썝꽦씤옄瑜 媛곴린 떎瑜닿쾶 蹂댁쑀븯怨 엳쑝硫(Kaper et al., 2004), 듅젙 媛먯뿼利앷낵 愿젴맂 寃껋쑝濡 蹂닿퀬릺怨 엳떎(Adamus-Bialek et al., 2009). 슂濡쒓컧뿼쓣 쑀諛쒗븯뒗 슂濡쒕퀝썝꽦 옣洹좎뿉꽌 쓷븯寃 솗씤릺뒗 O 삁泥삎 O1, O2, O4, O6, O7, O8, O15, O16, O18, O21, O22, O25, O75 洹몃━怨 O83 벑쑝濡 븣젮議뚯쑝硫(Ørskov et al., 1982; Johnson et al., 1994; Blanco et al., 1996; Bidet et al., 2007; Abe et al., 2008; Ananias and Yano, 2008), 슂濡쒕퀝썝꽦 옣洹좎씠 蹂댁쑀븯怨 엳뒗 蹂묒썝꽦씤옄뿉뒗 P-fimbriae, S fimbriae, type 1 fimbriae 媛숈 꽭룷遺李⑹씤옄, α-hemolysin, cytotoxic necrotizing factor type 1 벑쓽 룆냼 bacteriocin 諛 siderophores 벑씠 븣젮졇 엳떎(Adamus-Bialek et al., 2009). 슂濡쒕퀝썝꽦 옣洹좎뿉 쓽븳 슂濡쒓컧뿼쓽 꽦由쎌뿉뒗 P fimbriae 媛숈 꽭룷遺李⑹씤옄瑜 씠슜븯뿬 슂濡쒖긽뵾꽭룷뿉 遺李⑺븯硫, 냼蹂뿉 쓽븳 슂濡쒖쓽 꽭泥숈옉슜뿉 빆븯뿬 媛먯뿼뿉 寃곗젙쟻씤 뿭븷쓣 븳떎(He et al., 2015). 옣洹좎 A, B1, B2, D 벑 4媛吏 二쇱슂 怨꾪넻遺꾨쪟援(phylogenetic group)쑝濡 遺꾨쪟媛 媛뒫븯硫 옣愿 씠쇅쓽 媛먯뿼利앹쓣 쑀諛쒗븯뒗 蹂묒썝꽦 옣洹좎 二쇰줈 B2 D援곗뿉 냽븳떎(Clermont et al., 2000).

빐쇅쓽 寃쎌슦 슂濡쒕퀝썝꽦 옣洹좎쓽 O 삁泥삎怨 蹂묒썝꽦씤옄 諛 怨꾪넻遺꾨쪟援곗쓽 뿰愿꽦뿉 븳 뿰援ш 솢諛쒗엳 닔뻾릺怨 엳쑝굹(Blanco et al., 1996; Clermont et al., 2000; Ananias and Yano, 2008; Baldy-Chudzik et al., 2008; Bukh et al., 2009), 援궡뿉꽌뒗 슂濡쒕퀝썝꽦 옣洹좎쓽 O 삁泥삎뿉 뵲瑜 蹂묒썝꽦씤옄 諛 怨꾪넻遺꾨쪟援곗뿉 븳 뿰援щ 李얠븘蹂닿린 옒뱾떎.

뵲씪꽌 蹂 뿰援ъ뿉꽌뒗 1989뀈룄 2010뀈룄遺꽣 2014뀈룄뿉 遺꾨━맂 슂濡쒕퀝썝꽦 옣洹좎쓣 긽쑝濡 O-serogroup怨 蹂묒썝꽦씤옄 諛 怨꾪넻遺꾨쪟援곗쓣 遺꾩옄깮臾쇳븰쟻 諛⑸쾿쓣 씠슜븯뿬 鍮꾧탳 遺꾩꽍븿쑝濡쒖뜥 援궡뿉꽌 遺꾨━릺뒗 슂濡쒕퀝썝꽦 옣洹좎쓽 O-serogroup뿉 뵲瑜 蹂묒썝꽦씤옄 蹂댁쑀쑉 諛 怨꾪넻遺꾨쪟援곗뿉 븳 뿭븰쟻 湲곗큹옄猷뚮 젣떆븯怨좎옄 븯떎.

옱猷 諛 諛⑸쾿

蹂 뿰援ъ뿉꽌뒗 媛뺤썝룄 냼옱쓽 븳 븰蹂묒썝뿉꽌 1989뀈 3썡遺꽣 12썡源뚯 닔吏묓븳 슂濡쒕퀝썝꽦 옣洹 123二쇱 2010뀈 7썡遺꽣 2014뀈 7썡源뚯 닔吏묓븳 슂濡쒕퀝썝꽦 옣洹 558二쇰 蹂 뿰援ъ뿉 궗슜븯떎.

슂濡쒕퀝썝꽦 옣洹좎쓽 DNA 異붿텧

슂濡쒕퀝썝꽦 옣洹좎쓣 brain heart infusion broth뿉 젒醫낇븯뿬 37℃뿉꽌 18떆媛 諛곗뼇 썑, 꽭洹 諛곗뼇븸 1 mL쓣 12,000 rpm뿉꽌 10遺꾧컙 썝떖遺꾨━븯怨 닔꽭븯뒗 怨쇱젙쓣 2쉶 諛섎났븯떎. 꽭洹좎묠궗뿉 200 μL쓽 硫멸퇏利앸쪟닔瑜 꽔뼱 꽭洹좊쑀븸쓣 젣議고븯怨, 100℃뿉꽌 10遺꾧컙 걪씤 떎쓬, 12,000 rpm뿉꽌 10遺꾧컙 썝떖遺꾨━븯뿬 긽痢듭븸쓣 二쇳삎 DNA濡 궗슜븯떎.

Multiplex polymerase chain reaction (PCR)쓣 씠슜븳 O-serogroup쓽 寃異

슂濡쒕퀝썝꽦 옣洹좎쓽 O-serogroup瑜 솗씤븯湲 쐞븳 multiplex PCR뿉 궗슜븳 primer뒗 CosmoGenetech Co. (Seoul, Korea)뿉 쓽猶고븯뿬 빀꽦븯쑝硫 primer쓽 뿼湲곗꽌뿴怨 利앺룺맂 궛臾쇱쓽 겕湲곕뒗 Table 1怨 媛숇떎(Li et al., 2010). 30 μL쓽 reaction mixture뿉뒗 異붿텧븳 옣洹좎쓽 DNA 2 μL, 媛 냽룄蹂 primer (Table 1), 10 X PCR buffer, 0.3 mM deoxynucleoside triphosphate (dATP, dGTP, dCTP, dTTP), 2 U쓽 G-Taq DNA polymerase (CosmoGenetech Co., Seoul, Korea)瑜 샎빀븯떎. 以묓빀슚냼 뿰뇙諛섏쓳 95℃뿉꽌 5遺꾧컙 denaturation쓣 떎떆븯怨, 95℃뿉꽌 30珥, 55℃뿉꽌 1遺, 72℃뿉꽌 1遺꾧컙 30 cycle쓣 븳 썑, 72℃뿉꽌 5遺꾧컙 final extension쓣 닔뻾븯떎. 利앺룺궛臾쇱 2.0% agarose gel뿉꽌 100 bp DNA ladder (Fermentas, Burlington, Canada) 븿猿 1 X TAE buffer뿉꽌 쟾湲곗쁺룞 븳 썑, ethidium bromide (0.5 μg/mL)濡 10遺꾧컙 뿼깋븯뿬 GelDoc Image Analyzer (Biorad, Hercules, USA)瑜 씠슜븯뿬 利앺룺 쑀臾 諛 겕湲곕 愿李고븯떎.

Oligonucleotide sequences for O-serotyping of UPEC

Serogroup Primer Primer sequence (5'-3') Amplicon size (bp) Target gene Conc. in multiplex PCR (μM) Reference
Group 1 Li et al., 2010
O1 wl-14632-(F) GTGAGCAAAAGTGAAATAAGGAACG 1,098 wzx 0.05
wl-14633-(R) CGCTGATACGAATACCATCCTAC
O6 wl-14646-(F) GGATGACGATGTGATTTTGGCTAAC 783 wzy 0.07
wl-14647-(R) TCTGGGTTTGCTGTGTATGAGGC
O7 wl-14648-(F) CTATCAAAATACCTCTGCTGGAATC 610 wzy 0.12
wl-14649-(R) TGGCTTCGAGATTAAACCTATTCCT
O8 wl-14652-(F) CCAGAGGCATAATCAGAAATAACAG 448 orf469 0.12
wl-14653-(R) GCAGAGTTAGTCAACAAAAGGTCAG
O16 wl-14654-(F) GGTTTCAATCTCACAGCAACTCAG 302 wzx 0.13
wl-14655-(R) GTTAGAGGGATAATAGCCAAGCGG
O21 wl-14676-(F) CTGCTGATGTCGCTATTATTGCTG 209 wzx 0.12
wl-14677-(R) TGAAAAAAAGGGAAACAGAAGAGCC
O75 wl-17413-(F) GAGATATACATGGGGAGGTAGGCT 511 wzx 0.07
wl-17414-(R) ACCCGATAATCATATTCTTCCCAAC
Group 2
O2 wl-14636-(F) AGTGAGTTACTTTTTAGCGATGGAC 770 wzy 0.07
wl-14637-(R) AGTTTAGTATGCCCCTGACTTTGAA
O4 wl-14642-(F) TTGTTGCGATAATGTGCATGTTCC 664 wzy 0.07
wl-14643-(R) AATAATTTGCTATACCCACACCCTC
O15 wl-14672-(F) TCTTGTTAGAGTCATTGGTGTATCG 183 wzy 0.08
wl-14673-(R) ATAAAACGAGCAAGCACCACACC
O18 wl-14656-(F) GTTCGGTGGTTGGATTACAGTTAG 551 wzx 0.08
wl-14657-(R) CTACTATCATCCTCACTGACCACG
O22 wl-14660-(F) TTCATTGTCGCCACTACTTTCCG 468 wzx 0.08
wl-14661-(R) GAAACAGCCCATGACATTACTACG
O25 wl-14666-(F) AGAGATCCGTCTTTTATTTGTTCGC 230 wzy 0.08
wl-14667-(R) GTTCTGGATACCTAACGCAATACCC
O83 wl-14668-(F) GTACACCAGGCAAACCTCGAAAG 362 wzx 0.08
wl-14669-(R) TTCTGTAAGCTAATGAATAGGCACC


Multiplex PCR쓣 씠슜븳 蹂묒썝꽦씤옄쓽 寃異

떎뿕뿉 궗슜븳 primer뒗 CosmoGenetech Co. (Seoul, Korea)뿉 쓽猶고븯뿬 빀꽦븯쑝硫 primer쓽 뿼湲곗꽌뿴 諛 利앺룺맂 궛臾쇱쓽 겕湲곕뒗 Table 2 媛숇떎(Adamus-Bialek et al, 2009). Multiplex PCR쓣 씠슜븯뿬 蹂묒썝꽦씤옄瑜 寃異쒗븯湲 쐞빐 30 μL쓽 reaction mixture뿉뒗 異붿텧븳 옣洹좎쓽 DNA 2 μL, 5 pmole쓽 媛 primer, 10 X PCR buffer, 0.2 mM deoxynucleoside triphosphate, 1 U G-Taq DNA polymerase (CosmoGenetech Co., Seoul, Korea)瑜 샎빀븯떎. 以묓빀슚냼 뿰뇙諛섏쓳 thermal cycler (GeneAmp PCR system 2700, Perkin-Elmer Cetus, Boston, USA)瑜 씠슜븯뿬 95℃뿉꽌 5遺꾧컙 denaturation쓣 떎떆븯怨, 95℃뿉꽌 1遺, 60℃뿉꽌 1遺 30珥, 72℃뿉꽌 3遺꾧컙 35 cycle쓣 븳 썑, 72℃뿉꽌 8遺꾧컙 final extension쓣 닔뻾븯떎. 利앺룺궛臾쇱 1.5% agarose gel뿉꽌 100 bp DNA ladder (Fermentas, Burlington, Canada) 븿猿 1 X TAE buffer뿉꽌 쟾湲곗쁺룞 븳 썑, ethidium bromide (0.5 μg/mL)濡 10遺꾧컙 뿼깋븯뿬 GelDoc Image Analyzer (Biorad, Hercules, USA)瑜 씠슜븯뿬 利앺룺 쑀臾 諛 利앺룺궛臾쇱쓽 겕湲곕 愿李고븯떎.

Oligonucleotide sequences for amplification of the virulence factors genes and phylogenetic groups

Primer Primer sequence (5'-3') Amplicon size (bp) Target gene Reference
VFs pap1 GACGGCTGTACTGCAGGGTGTGGCG 328 papC Adamus-Bialek et al., 2009
pap2 ATATCCTTTCTGCAGGGATGCAATA
sfa1 CTCCGGAGAACTGGGTGCATCTTAC 410 sfaD/sfaE
sfa2 CGGAGGAGTAATTACAAACCTGGCA
cnf1a AAGATGGAGTTTCCTATGCAGGAG 498 cnf1
cnf12a CATTCAGAGTCCTGCCCTCATTATT
usp1mod TTCTGGGGAACTGACATTCACGG 657 usp
usp2mod CCTCAGGGACATAGGGGGAA
fimGH1 GCAATGTTGGCGTTCGCAAGTGC 1,001 fimG/fimH
fimGH2 CGTAAATATTCCACACAAACTGG
hly1mod AACAACGATAAGCACTGTTCTGGCT 1,177 hlyA
hly2mod ACCATATAAGCGGTCATTCCCATCA
PGs TspE4C1 GAGTAATGTCGGGGCATTCA 152 TSPE4.C2 Clermont et al., 2000
TspE4C2 CGCGCCAACAAAGTATTACG
YjA1 TGAAGTGTCAGGAGACGCTG 211 yjaA
YjA2 ATGGAGAATGCGTTCCTCAAC
ChuA1 GACGAACCAACGGTCAGGAT 279 chuA
ChuA2 TGCCGCCAGTACCAAAGACA

Abbreviation: VFs, virulence factor; PG, phylogenetic group



Multiplex PCR쓣 씠슜븳 怨꾪넻遺꾨쪟援 솗씤

怨꾪넻遺꾨쪟援곗쓣 솗씤븯湲 쐞빐 떎뿕뿉 궗슜븳 primer쓽 뿼湲곗꽌뿴 Table 2 媛숇떎(Clermont et al., 2000). 20 μL쓽 reaction mixture뿉뒗 異붿텧븳 슂濡쒕퀝썝꽦 옣洹좎쓽 DNA 2 μL, 10 pmole쓽 媛 primer, 0.2 mM deoxynucleoside triphosphate, 2.5 U G-Taq DNA polymerase (CosmoGenetech Co., Seoul, Korea)瑜 샎빀븯떎. 以묓빀슚냼 뿰뇙諛섏쓳 thermal cycler (GeneAmp PCR system 2700, Perkin-Elmer Cetus, Boston, USA)瑜 씠슜븯뿬 94℃뿉꽌 4遺꾧컙 denaturation븳 썑, 94℃뿉꽌 5珥, 59℃뿉꽌 10珥, 72℃뿉꽌 5遺꾧컙 30 cycle쓣 닔뻾븯떎. 利앺룺궛臾쇱 1.5% agarose gel뿉꽌 100 bp DNA ladder (Fermentas, Burlington, Canada) 븿猿 1 X TAE buffer瑜 궗슜븯뿬 쟾湲곗쁺룞 븳 썑 ethidium bromide (0.5 μg/mL)濡 10遺꾧컙 뿼깋븯뿬 GelDoc Image Analyzer (Biorad, Hercules, USA)瑜 씠슜븯뿬 利앺룺 쑀臾 諛 利앺룺궛臾쇱쓽 겕湲곕 愿李고븯떎.

寃 怨

슂濡쒕퀝썝꽦 옣洹좎쓽 O-serogroup 寃異

1989뀈룄 2010-2014뀈룄뿉 遺꾨━븳 슂濡쒕퀝썝꽦 옣洹좎쓽 O-serogroup쓽 遺꾪룷뒗 Table 5 媛숇떎. 슂濡쒕퀝썝꽦 옣洹좎쓽 O-serogroup쓣 솗씤븯湲 쐞븳 multiplex PCR 諛⑸쾿쑝濡 O-serogroup媛 寃異쒕릺吏 븡 寃쎌슦媛 1989뀈룄 遺꾨━二쇱뿉꽌 123二 以 55二(44.7%), 2010-2014뀈룄 遺꾨━二쇱뿉꽌 558二 以 136二 (24.4%)怨, 씠瑜 젣쇅븯硫 1989뀈룄 遺꾨━二쇱뿉꽌 68二 (55.3%), 2010-2014뀈룄 遺꾨━二쇱뿉꽌 422二 (75.6%)뿉꽌 O1, O2, O4, O6, O7, O8, O15, O16, O18, O21, O22, O75 諛 O83뿉 빐떦븯뒗 O-serogroup쓣 솗씤븷 닔 엳뿀떎. 1989뀈룄 遺꾨━二쇱뿉꽌뒗 O15, O16, O18 諛 O22뒗 솗씤릺吏 븡븯쑝硫, O6 (11.1%), O25 O75 (9.8%), O8 (7.1%), O1 (4.8%), O21 (3.2%), O2 O4 (2.4%), 07 (0.8%)쓽 鍮덈룄濡 굹궃 諛섎㈃, 2010-2014뀈룄 遺꾨━二쇱뿉꽌뒗 O25 (21.0%), O2 (12.4%), O75 (10.8%), O6 (7.7%), O1 (7.2%), O8 (3.6%), O15 (3.4%), O18 (2.7%), O16 (2.5%), O4 (1.6%), O22 O7 (0.9%), O21 O83 (0.5%)濡 솗씤릺뿀떎.

O-serogroup, virulence factors and phylogenetic group of uropathogenic E. coli isolated from patient with urinary tract infections in 1989 and 2010-2014

O-serogroup Year Virulence factors Phylogenetic group
fimG/H sfaD/E papC usp cnf1 hlyA A B1 B2 D Total
O1 1989 6 (100%) 2 (33.3%) 2 (33.3%) 5 (83.3%) - - - - - 6 6 (4.9%)
2010-2014 36 (90%) 4 (10%) 12 (57.5%) 3 (7.5%) 8 (20%) 4 (10%) - - 20 20 40 (7.2%)
O2 1989 3 (100%) 3 (100%) 1 (33.3%) 2 (66.7%) 3 (100%) 2 (66.7%) - - 3 - 3 (2.4%)
2010-2014 68 (98.6%) 19 (27.5%) 63 (91.3%) 8 (11.6%) 24 (34.8%) 3 (4.3%) - - 65 4 69 (12.4%)
O4 1989 2 (100%) 2 (100%) 2 (100%) 2 (100%) 2 (100%) 1 (57.1%) 1 - 2 - 3 (2.4%)
2010-2014 9 (100%) 5 (55.6%) 4 (44.4%) 4 (44.4%) 5 (55.6%) 3 (33.3%) 1 2 6 - 9 (1.6%)
O6 1989 11 (78.6%) 8 (57.1%) 8 (57.1%) 9 (64.3%) 8 (57.1%) 8 (57.1%) 5 - 9 - 14 (11.4%)
2010-2014 43 (100%) 30 (69.8%) 27 (62.8%) 26 (60.5%) 30 (69.8%) 25 (58.1%) 2 2 30 9 43 (7.7%)
O7 1989 1 (100%) - - - - - - - - 1 1 (0.8%)
2010-2014 3 (60%) 1 (20%) 1 (20%) 1 (20%) - - 2 - 1 2 5 (0.9%)
O8 1989 6 (66.7%) - 1 (11.1%) - - - 2 2 1 4 9 (7.3%)
2010-2014 17 (85%) 3 (15%) 1 (5%) 2 (10%) 2 (10%) - 5 6 7 2 20 (3.6%)
O15 1989 - - - - - - - - - - -
2010-2014 17 (89.5%) 1 (5.3%) 9 (47.4%) 3 (15.8%) 8 (42.1%) 1 (5.3%) 4 - 2 13 19 (3.4%)
O16 1989 - - - - - - - - - - -
2010-2014 14 (100%) - 4 (28.6%) - - 1 (7.1%) 2 - 9 3 14 (2.5%)
O18 1989 - - - - - - - - - - -
2010-2014 15 (100%) 2 (13.3%) 3 (20%) 6 (40%) 4 (26.7%) 3 (20%) 3 1 6 5 15 (2.7%)
O21 1989 4 (100%) - 4 (100%) 3 (75.0%) - 4 (100%) - - 4 - 4 (3.3%)
2010-2014 3 (100%) 1 (33.3%) 1 (33.3%) - 1 (33.3%) 1 (33.3%) 1 1 - 1 3 (0.5%)
O22 1989 - - - - - - - - - - -
2010-2014 5 (100%) - 1 (20%) 2 (40%) - - - - 2 3 5 (0.9%)
O25 1989 12 (100%) - - 2 (16.7%) - - - - 9 3 12 (9.8%)
2010-2014 110 (94%) 12 (10.3%) 37 (31.6%) 10 (8.5%) 24 (20.5%) 17 (14.5%) 7 5 96 9 117 (21.0%)
O75 1989 12 (100%) 1 (8.3%) 6 (50.0%) 8 (66.7%) 1 (8.3%) 2 (16.7%) 1 1 8 2 12 (9.8%)
2010-2014 57 (95%) 1 (1.7%) 2 (3.3%) 56 (93.3%) 10 (16.7%) - 1 - 57 2 60 (10.8%)
O83 1989 4 (100%) - - 2 (50.0%) - - - - 4 - 4 (3.3%)
2010-2014 2 (66.7%) 1 (33.3%) 1 (33.3%) 1 (33.3%) - - 1 - 2 - 3 (0.5%)
Other serogroup 1989 42 (76.4%) - 2 (3.6%) 10 (18.2%) - 2 (3.6%) 21 8 7 19 55 (44.7%)
2010-2014 118 (86.8%) 19 (14%) 31 (22.8%) 30 (22.1%) 30 (22.1%) 10 (7.4%) 27 13 42 54 136 (24.4%)


蹂묒썝꽦씤옄 怨꾪넻遺꾨쪟援 寃異

1989뀈룄 2010-2014뀈룄뿉 遺꾨━맂 슂濡쒕퀝썝꽦 옣洹좎쓽 蹂묒썝꽦 씤옄 怨꾪넻遺꾨쪟援곗쓽 鍮덈룄뒗 Table 3怨 媛숇떎. 1989뀈룄 遺꾨━二쇱뿉꽌 蹂묒썝꽦 씤옄쓽 蹂댁쑀 鍮덈룄뒗 fimG/H (83.7%), usp (32.5%), hlyA (13.8%), papC (21.1%), sfaD/E (13.0%), cnf1 (11.4%) 닚쑝濡 굹궃 諛섎㈃, 2010-2014뀈룄 遺꾨━二쇱뿉꽌뒗 fimG/H (92.7%), papC (37.3%), usp (27.2%), cnf1 (26.2%), sfaD/E (17.7%), hlyA (12.2%)쓽 닚쑝濡 굹궗떎. fimG/H뒗 1989뀈룄 2010-2014뀈룄 遺꾨━二쇱뿉꽌 紐⑤몢 媛옣 넂寃 굹궗떎. 듅엳, cnf1쓽 寃쎌슦 1989뀈룄 遺꾨━二쇱뿉꽌 11.4%뜕 諛섎㈃, 2010-2014뀈룄 遺꾨━二쇱뿉꽌 26.2%濡 굹궗쑝硫, papC쓽 寃쎌슦 1989뀈룄 遺꾨━二쇱뿉꽌 21.1%, 2010-2014뀈룄 遺꾨━二쇱뿉꽌 37.4%濡 솗씤릺뼱 1989뀈룄 遺꾨━二쇱뿉 鍮꾪빐 2010-2014遺꾨━二쇱뿉꽌 cnf1怨 papC媛 媛곴컖 14.8%, 16.2% 利앷븳 寃껋쑝濡 솗씤릺뿀떎. 怨꾪넻遺꾨쪟援곗쓽 寃쎌슦 1989뀈룄 遺꾨━二쇱쓽 39.8% 2010-2014뀈룄 遺꾨━二쇱쓽 61.6%媛 B2援곗뿉 냽븯뒗 寃껋쑝濡 솗씤릺뿀쑝硫, 1989뀈룄 遺꾨━二쇱쓽 26.8%, 2010-2014뀈룄 遺꾨━二쇱쓽 22.8%媛 D援곗뿉 냽븯뒗 寃껋쑝濡 솗씤릺뼱 B2 D援곗뿉 냽븯뒗 슂濡쒕퀝썝꽦 옣洹좎씠 1989뀈룄 遺꾨━二쇱뿉꽌 66.7%, 2010-2014뀈룄 遺꾨━二쇱뿉꽌 84.4%濡 솗씤릺뿀떎. 諛섎㈃, 蹂묒썝꽦씠 떎냼 궙 寃껋쑝濡 븣젮吏 A援곌낵 B1援곗뿉 냽븯뒗 슂濡쒕퀝썝꽦 옣洹좎 1989뀈룄 遺꾨━二쇱뿉꽌 24.4% 8.9%, 2010-2014뀈룄 遺꾨━二쇱뿉꽌 10% 5.6%濡 솗씤릺뼱 A援곌낵 B1援곗뿉 냽븯뒗 슂濡쒕퀝썝꽦 옣洹좎쓽 鍮덈룄媛 媛곴컖 14.4%, 3.3% 媛먯냼븳 寃껋쑝濡 솗씤릺뿀떎.

Frequency of virulence factor genes and phylogenetic groups of E. coli strains from patients with urinary tract infection isolated in 1989 and 2010-2014

Target Strains isolated in
1989 (n=123) 2010-2014 (n=558)
VFs hlyA 17 (13.8%) 68 (12.2%)
fimG/H 103 (83.7%) 517 (92.7%)
usp 40 (32.5%) 152 (27.2%)
cnf1 14 (11.4%) 146 (26.2%)
papC 26 (21.1%) 208 (37.3%)
sfaD/E 16 (13.0%) 99 (17.7%)
PGs A 30 (24.4%) 56 (10%)
B1 11 (8.9%) 31 (5.6%)
B2 49 (39.8%) 344 (61.6%)
D 33 (26.8%) 127 (22.8%)

Abbreviation: VFs, vrulence factor; PG, phylogenetic group



슂濡쒕퀝썝꽦 옣洹좎쓽 O-serogroup, 蹂묒썝꽦씤옄 諛 怨꾪넻遺꾨쪟援곗쓽 뿰愿꽦

蹂묒썝꽦씤옄 profile뿉 뵲瑜 怨꾪넻遺꾨쪟援 遺꾪룷뒗 Table 4 媛숇떎. 珥 6媛쒖쓽 virulence factors 以(fimG/H, sfaD/E, papC, usp, cnf1 and hlyA) type 1 fimbriae留 (fimG/H)쓣 蹂댁쑀븳 슂濡쒕퀝썝꽦 옣洹좎씠 媛옣 留롮씠 솗씤릺뿀쑝硫(1989뀈룄 遺꾨━二: 43.9%, 2010-2014뀈룄 遺꾨━二: 34.2%), type 1 fimbriae留(fimG/H)쓣 蹂댁쑀븳 E. coli瑜 젣쇅븯怨, 슂濡쒓컧뿼솚옄뿉꽌 遺꾨━븳 슂濡쒕퀝썝꽦 옣洹좎뿉꽌 넂 鍮덈룄瑜 蹂댁씠뒗 蹂묒썝꽦씤옄 profile 1989뀈룄 遺꾨━二쇱쓽 寃쎌슦, fimG/H -, sfaD/E -, papC -, usp -, cnf1 -, hlyA - (14.6%), fimG/H+, usp + (13.8%), fimG/H +, papC +, usp + (6.5%), fimG/H +, sfaD/E +, papC +, usp +, cnf1 +, hlyA + (5.7%), fimG/H +, papC +, usp +, hlyA + (3.3%), fimG/H +, papC +, hlyA + (2.4%) 닚씠뿀뜕 諛섎㈃, 2010-2014 遺꾨━二쇱쓽 寃쎌슦 fimG/H +, papC + (14.7%), fimG/H +, usp + (12.2%), fimG/H +, sfaD/E +, papC +, usp +, cnf1 +, hlyA + (5.6%), fimG/H -, sfaD/E -, papC -, usp -, cnf1 -, hlyA - (4.3%), fimG/H +, papC +, cnf1 + (3.9%), fimG/H +, cnf1 + (3.6%), fimG/H +, sfaD/E +, papC +, cnf1 + (3.0%) 닚씠뿀떎. 삉븳 蹂묒썝꽦씤옄瑜 븯굹 씠긽 媛吏뒗 슂濡쒕퀝썝꽦 옣洹좎 怨꾪넻遺꾨쪟援 A B1쓽 寃쎌슦 1989뀈룄 遺꾨━二쇱뿉꽌 22.8%怨, 2010-2014뀈룄 遺꾨━二쇱뿉꽌 12.9%뜕 諛섎㈃, 怨꾪넻遺꾨쪟援 B2 D쓽 寃쎌슦뒗 1989뀈룄 遺꾨━二쇱뿉꽌 62.6%, 2010-2014뀈룄 遺꾨━二쇱뿉꽌 82.8%떎. 1989뀈룄 遺꾨━二쇱뿉꽌 蹂묒썝꽦 씤옄瑜 媛옣 留롮씠 蹂댁쑀븯怨 엳뒗 O-serogroup O4怨, 蹂묒썝꽦씤옄 蹂댁쑀쑉 fimG/H (100%), sfaD/E (100%), papC (100%), usp (100%), cnf1 (100%), hlyA (57.1%)濡 굹궗쑝硫, 3二 以 2二(66.7%)媛 怨꾪넻遺꾨쪟援 B2뿉 냽븯떎(Table 5). 삉븳 2010-2014뀈룄 遺꾨━二쇱뿉꽌뒗 O6쓽 蹂묒썝꽦 씤옄 蹂댁쑀쑉씠 fimG/H (100%), sfaD/E (69.8%), papC (62.8%), usp (60.5%), cnf1 (69.8%), hlyA (58.1%)濡 媛옣 넂븯怨, 43二 以 39二(90.7%)媛 怨꾪넻遺꾨쪟援 B2 D援곗뿉 냽븯떎(Table 5).

Virulence factor gene profiles and phylogenetic groups of E. coli strains from patients with urinary tract infection isolated in 1989 and 2010-2014

1989 2010-2014
VF genotype Phylogenetic group VF genotype Phylogenetic group
F S P U C H A B1 B2 D n=123 F S P U C H A B1 B2 D n=558
+ - - - - - 10 11 13 20 54 (43.9%) + - - - - - 28 29 72 62 191 (34.2%)
+ - + - - - 1 1 (0.8%) + - + - - - 64 18 82 (14.7%)
+ - - + - - 6 6 5 17 (13.8%) + - - + - - 6 54 8 68 (12.2%)
+ + + + + + 7 7 (5.7%) + + + + + + 1 30 31 (5.6%)
- - - - - - 13 1 4 18 (14.6%) - - - - - - 15 5 4 24 (4.3%)
+ - + - + - 16 6 22 (3.9%)
+ - - - + - 1 7 12 20 (3.6%)
+ + + - + - 1 1 (0.8%) + + + - + - 1 16 17 (3.0%)
+ - + - + + 11 2 13 (2.3%)
+ - - + + - 1 1 (0.8%) + - - + + - 1 9 10 (1.8%)
+ + - - - - 1 7 1 9 (1.6%)
+ - + + - - 7 1 8 (6.5%) + - + + - - 1 3 3 7 (1.3%)
+ + + - + + 1 1 (0.8%) + + + - + + 5 1 6 (1.1%)
+ + - + + + 2 2 (1.6%) + + - + + + 6 6 (1.1%)
+ + - + - - 2 2 (1.6%) + + - + - - 6 6 (1.1%)
+ + + - - - 5 5 (0.9%)
- - - - + - 3 3 (0.5%)
+ + - + + - 1 1 (0.8%) + + - + + - 4 4 (0.7%)
+ + + + - - 5 5 (0.9%)
+ + + + + - 4 4 (0.7%)
- - - + - - 4 4 (0.7%)
- + + - + - 2 1 3 (0.5%)
- - + - - - 1 1 2 (0.4%)
+ - + + + + 2 2 (0.4%)
+ - - - - + 1 1 (0.8%) + - - - - + 1 1 (0.2%)
+ - - + - + 1 1 (0.2%)
+ - + - - + 3 3 (2.4%) + - + - - + 1 1 2 (0.4%)
+ - - - + + 1 1 (0.2%)
+ - + + + - 1 1 (0.2%)
- + + - - - 1 1 (0.2%)
+ + + + - + 1 1 (0.8%) + + + + - + 1 1 (0.2%)
+ + + + - - 1 1 (0.2%)
+ - + + + + 1 1 (0.2%)
- - - - - + 1 1 (0.2%)
+ - + + - + 4 4 (3.3%) + - + + - + 1 1 (0.2%)
- - + - - + 1 1 (0.2%)
- + - - + - 1 1 (0.2%)
- + + + + - 1 1 (0.8%)
Total 30 11 49 33 123 Total 56 31 344 127 558

Abbreviation: F, fimG/H; S, sfaD/E; P, papC; U, usp; C, cnf1; and H, hlyA


怨 李

슂濡쒓컧뿼 꽦蹂꾩뿉 愿怨꾩뾾씠 쑀諛쒕릺뒗 吏덈퀝쓽 몴쟻씤 寃껋쑝濡 듅엳 뿬꽦씠 궓꽦蹂대떎 뜑 留롮씠 媛먯뿼릺뒗 寃껋쑝濡 븣젮議뚮떎(Magliano et al., 2012). E. coli뿉 쓽븳 슂濡쒓컧뿼씠 媛옣 쓷븳 寃껋쑝濡 븣젮졇 엳쑝硫 슂濡쒓컧뿼쓣 쑀諛쒗븯뒗 E. coli瑜 슂濡쒕퀝썝꽦 옣洹(uropathogenic Escherichia coli, UPEC)씠씪 븳떎(Totsika et al., 2012). 슂濡쒕퀝썝꽦 옣洹좎 떎뼇븳 蹂묒썝꽦씤옄瑜 媛吏먯쑝濡쒖뜥 슂濡쒓컧뿼쓣 쑀諛쒗븷 닔 엳쑝硫 O-serogroup怨 怨꾪넻遺꾨쪟援곗뿉 뵲씪 蹂댁쑀븯怨 엳뒗 蹂묒썝꽦 씤옄媛 떎뼇븯寃 諛앺議뚮떎(Momtaz et al., 2013). 蹂 뿰援ъ뿉꽌뒗 1989뀈룄 2010뀈룄遺꽣 2014뀈룄源뚯 닔吏묐맂 슂濡쒕퀝썝꽦 옣洹좎쓽 O-serogroup, 蹂묒썝꽦씤옄 諛 怨꾪넻遺꾨쪟援곗쓽 鍮덈룄瑜 鍮꾧탳 遺꾩꽍 빐蹂닿퀬옄 븯떎. 1989뀈룄뿉 遺꾨━븳 슂濡쒕퀝썝꽦 옣洹 123二쇱 2010뀈룄遺꽣 2014뀈룄源뚯 遺꾨━븳 슂濡쒕퀝썝꽦 옣洹 558二쇱뿉 븯뿬 슂濡쒕퀝썝꽦 옣洹좎뿉꽌 二쇰줈 遺꾨━릺뒗 O-serogroup씤 O1, O2, O4, O6, O7, O8, O15, O16, O18, O21, O22, O25, O75 洹몃━怨 O83쓽 鍮덈룄瑜 솗씤븯湲 쐞빐 flippase gene씤 wzx gene怨 polymerase gene씤 wzy gene뿉 븳 multiplex-PCR쓣 닔뻾븯떎. 蹂 뿰援ъ뿉꽌 O-serogroup瑜 솗씤븯湲 쐞빐 궗슜븳 multiplex PCR濡 寃곗젙븷 닔 뾾뜕 寃쎌슦瑜 젣쇅븯硫 O1, O2, O4, O6, O7, O8, O15, O16, O18, O21, O22, O25, O75 諛 O83뿉 빐떦븯뒗 O-serogroup 쟾泥 슂濡쒕퀝썝꽦 옣洹좎쓽 怨쇰컲닔 씠긽씠뿀떎(1989뀈룄 遺꾨━二: 55.3%, 2010-2014뀈룄 遺꾨━二: 75.6%). 듅엳, 슂濡쒕퀝썝꽦 옣洹좎뿉꽌 쓷엳 굹굹뒗 O1遺꽣 O83源뚯 14醫낆쓽 O-serogroup뿉 빐떦븯뒗 洹좎<쓽 鍮꾩쑉씠 1989뀈룄뿉 鍮꾪빐 2010-2014뀈룄뿉 遺꾨━맂 슂濡쒕퀝썝꽦 옣洹좎쓽 鍮꾩쑉씠 20.3% 利앷븳 寃껋쑝濡 솗씤릺뿀떎. 1989뀈룄뿉 遺꾨━맂 슂濡쒕퀝썝꽦 옣洹좎쓽 寃쎌슦 O15, O16, O18 洹몃━怨 O22뒗 솗씤릺吏 븡븯쑝硫, 媛옣 留롮 鍮덈룄瑜 굹궦 O-serogroup O6濡 123二 以 14二(11.4%)瑜 李⑥븯怨, O25 O75媛 媛곴컖 12二(9.8%)濡 넂 鍮덈룄瑜 蹂댁뿬 1989뀈룄뿉 쑀뻾븳 O-serogroup뒗 O6엫쓣 솗씤븯떎. 諛섎㈃ 2010뀈룄遺꽣 2014뀈룄源뚯 遺꾨━맂 슂濡쒕퀝썝꽦 옣洹좎쓽 寃쎌슦 O25媛 558二 以 117二(21.0%)濡 媛옣 넂 鍮덈룄瑜 蹂댁怨 O2媛 69二(12.4%), O75媛 60二(10.8%)쓽 鍮덈룄濡 굹굹, 1989뀈룄뿉 遺꾨━맂 슂濡쒕퀝썝꽦 옣洹좎쓽 O-serogroup怨쇰뒗 떎냼 李⑥씠媛 엳뿀떎. 듅엳 媛옣 留롮 鍮덈룄瑜 굹궦 O-serogroup씠 O6뿉꽌 O25濡 蹂븯쓬쓣 솗씤븯떎. Momtaz 벑 (2013) 슂濡쒓컧뿼 솚옄濡쒕꽣 123二쇱쓽 슂濡쒕퀝썝꽦 옣洹좎쓣 遺꾨━븯뿬 O-serogroup쓽 遺꾪룷瑜 솗씤븯뒗뜲, 珥 123二쇱쓽 슂濡쒕퀝썝꽦 옣洹 以 O25媛 32二(26.0%)濡 媛옣 쓷븳 O-serogroup엫쓣 蹂닿퀬븯뿬, 蹂 뿰援ъ쓽 寃곌낵 鍮꾩듂 븯떎(Momtaz et al., 2013). Blanco 벑 (1996)쓽 뿰援ъ뿉꽌뒗 몢 踰덉㎏濡 쓷엳 遺꾨━맂 O-serogroup씠 O6씪怨 蹂닿퀬븯뒗뜲, 蹂 뿰援ъ쓽 1989뀈룄 遺꾨━二쇱뿉꽌 媛옣 넂 鍮덈룄濡 굹궃 O-serogroup쓽 寃곌낵 鍮꾩듂븯떎(Blanco et al., 1996).

븳렪 multiplex-PCR쓣 씠슜븯뿬 蹂묒썝꽦씤옄쓽 蹂댁쑀쑉쓣 솗씤빐蹂 寃곌낵, 1989뀈룄 遺꾨━二쇱뿉꽌 fimG/H (83.7%), sfaD/E (13.0%), papC (21.1%), usp (32.5.0%), cnf1 (11.4%), hlyA (13.8%)濡 굹궃 諛섎㈃, 2010-2014뀈룄 遺꾨━二쇱뿉꽌뒗 fimG/H (92.7%), sfaD/E (17.7%), papC (37.3%), usp (27.2%), cnf1 (26.2%), hlyA (12.2%)濡 媛곴컖 솗씤릺뿀떎. 듅엳, 媛옣 쓷엳 蹂댁쑀븯怨 엳뒗 蹂묒썝꽦씤옄뒗 fimG/H (type 1 fimbriae)씤 寃껋쑝濡 솗씤릺뿀떎. Adamus-Bialek 벑 (2009) 127二쇱쓽 슂濡쒕퀝썝꽦 옣洹 以 122二(96.1%)뿉꽌 type 1 fimbriae瑜 蹂댁쑀븯떎怨 븯怨(Adamus-Bialek et al., 2009), Yun 벑 (2014) 뼱由곗씠쓽 슂濡쒓컧뿼 諛 臾댁쬆긽꽭洹좊눊瑜 쑀諛쒗븳 슂濡쒕퀝썝꽦 옣洹 64二 以 62二(96.9%)뿉꽌 type 1 fimbriae (fimH)쓣 蹂댁쑀븯떎怨 蹂닿퀬(Yun et al., 2014)븯뒗뜲, 씠뒗 蹂 뿰援ъ뿉꽌 굹궃 슂濡쒕퀝썝꽦 옣洹좎쓽 type 1 fimbriae 蹂댁쑀쑉怨 鍮꾩듂븯떎. 삉븳 蹂묒썝꽦씤옄 媛옣 諛젒븳 뿰愿씠 엳뒗 寃껋쑝濡 蹂댁씠뒗 O-serogroup 1989뀈룄 遺꾨━二쇱쓽 寃쎌슦 O4怨, 蹂묒썝꽦씤옄쓽 蹂댁쑀쑉 hlyA (57.1%)瑜 젣쇅븳 fimG/H, sfaD/E, papC, usp, cnf1쓽 蹂댁쑀쑉씠 100%濡 굹궗떎. 諛섎㈃, 2010-2014뀈룄 遺꾨━二쇱뿉꽌뒗 O6媛 蹂묒썝꽦씤옄 媛옣 諛젒븳 뿰愿꽦씠 엳뒗 寃껋쑝濡 솗씤릺뿀떎(fimG/H: 100%, sfaD/E: 69.8%, papC: 62.8%, usp: 60.5%, cnf1: 69.8% and hlyA: 58.1%). fimG/H, papC, cnf1 諛 sfaD/E쓽 蹂댁쑀쑉 1989뀈룄 遺꾨━二 蹂대떎 2010-2014뀈룄 遺꾨━二쇱뿉꽌 뜑 넂寃 굹궗뒗뜲, 씠뒗 fimG/H, papC, cnf1 諛 sfaD/E瑜 蹂댁쑀븯뒗 O15, O16, O18, O22뿉 빐떦븯뒗 O-serogroup씠 1989뀈룄뿉 遺꾨━릺吏 븡븯뜕 諛섎㈃, 2010-2014뀈룄 遺꾨━二쇱뿉꽌留 遺꾨━릺뿀湲 븣臾몄씠씪 궗猷뚮맂떎.

Clermont 벑 옣쇅 蹂묒썝꽦쓣 굹궡뒗 E. coli쓽 遺遺꾩씠 怨꾪넻遺꾨쪟援 B2 D援곗뿉 냽븳떎怨 븯떎(Clermont et al., 2000). 蹂 뿰援ъ쓽 怨꾪넻遺꾨쪟援 遺꾩꽍 寃곌낵瑜 궡렣蹂대㈃, 怨꾪넻遺꾨쪟援 B2뿉 냽븯뒗 寃쎌슦媛 1989뀈룄 遺꾨━二 39.8%, 2010-2014뀈룄 遺꾨━二 61.6%濡 솗씤릺뿀怨, 怨꾪넻遺꾨쪟援 D뿉 냽븯뒗 寃쎌슦媛 1989뀈룄 遺꾨━二 26.8%, 2010-2014뀈룄 遺꾨━二 22.8%濡 솗씤릺뼱, B2 D援곗쓽 鍮덈룄媛 넂븯쑝硫 듅엳, 2010-2014뀈룄 遺꾨━二쇱뿉꽌 B2援곗뿉 냽븯뒗 鍮꾩쑉씠 1989뀈룄 遺꾨━二 蹂대떎 뜑 넂븯떎. 뜑슧씠, 븳 媛吏 씠긽쓽 蹂묒썝꽦씤옄瑜 蹂댁쑀븳 슂濡쒕퀝썝꽦 옣洹좎씠 B2 D援곗뿉 냽븯뒗 鍮꾩쑉씠 1989뀈룄 遺꾨━二쇱뿉꽌 62.6%뜕 諛섎㈃, 2010-2014뀈룄 遺꾨━二쇱뿉꽌 82.8%濡 솗씤릺뿀쑝硫 씠뒗 슂濡쒕퀝썝꽦 옣洹 58二 以 B2援곗뿉 냽븯뒗 寃쎌슦媛 46二(79.31%), D援곗뿉 냽븯뒗 寃쎌슦媛 9二(15.51%)씪怨 蹂닿퀬븳 Lee 벑쓽 뿰援 寃곌낵 鍮꾩듂븯떎(Lee et al., 2016).

蹂 뿰援ъ뿉꽌뒗 1989뀈룄 2010뀈룄遺꽣 2014뀈룄뿉 遺꾨━맂 슂濡쒕퀝썝꽦 옣洹좎쓣 긽쑝濡 O-serogroup怨 蹂묒썝꽦씤옄 諛 怨꾪넻遺꾨쪟援곗쓽 遺꾪룷 諛 뿰愿꽦쓣 醫낇빀쟻쑝濡 遺꾩꽍븯떎. 蹂 뿰援ъ쓽 寃곌낵瑜 醫낇빀쟻쑝濡 깮媛곹빐 蹂댁븯쓣 븣, 슂濡쒕퀝썝꽦 옣洹좎쓽 O-serogroup怨 蹂묒썝꽦씤옄 洹몃━怨 怨꾪넻遺꾨쪟援곗씠 諛젒븯寃 뿰愿씠 엳뒗 寃껋쑝濡 굹궗쑝硫, 듅엳 1989뀈룄 遺꾨━二쇱쓽 蹂묒썝꽦씤옄 蹂댁쑀쑉蹂대떎 2010-2014뀈룄 遺꾨━二쇱쓽 fimG/H, papC, sfaD/E 諛 cnf1쓽 蹂댁쑀쑉씠 뜑 넂寃 굹궗떎. 蹂묒썝꽦씤옄 諛젒븳 뿰愿씠 엳뒗 寃껋쑝濡 솗씤맂 O-serogroup뒗 O4 O6쑝硫, 怨꾪넻遺꾨쪟援 B2 D援곗뿉 냽븯뒗 슂濡쒕퀝썝꽦 옣洹좎쓽 鍮덈룄媛 넂븯떎. 슂濡쒕퀝썝꽦 옣洹좎뿉 쓽븳 슂濡쒓컧뿼뿉 븳 삁諛⑹쓽븰쟻 痢〓㈃뿉꽌 쟻젅븳 梨낆쓣 닔由쏀븯湲 쐞빐 蹂묒썝꽦씤옄瑜 넂 鍮덈룄濡 蹂댁쑀븯怨 엳뒗 슂濡쒕퀝썝꽦 옣洹좎쓽 O-serogroup뿉 븳 異붿쟻 諛 愿李곗쓣 쐞븳 吏냽쟻씤 紐⑤땲꽣留곸씠 븘슂븷 寃껋쑝濡 궗猷뚮맂떎. 븳렪, 蹂 뿰援ъ뿉꽌뒗 슂濡쒓컧뿼 醫낅쪟뿉 뵲瑜 遺꾩꽍 떆뻾릺吏 븡븯쑝誘濡, 슂濡쒓컧뿼 醫낅쪟蹂 슂濡쒕퀝썝꽦 옣洹좎쓽 O-serogroup쓽 遺꾪룷, 蹂묒썝꽦씤옄 蹂댁쑀쑉 諛 怨꾪넻遺꾨쪟援곗뿉 愿븳 뿰援щ 吏꾪뻾븳떎硫 뜑 굹 寃곕줎쓣 뼸쓣 닔 엳쑝由щ씪 깮媛곷맂떎.

ACKNOWLEDGEMENT

"This Work was supported by the Masan University research grant in 2021".

CONFLICT OF INTEREST

No potential conflict of interest relevant to this article was reported.

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