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Anti-Adipogenic Activity of Ailanthoidol on 3T3-L1 Adipocytes
Published online June 30, 2014
© 2014 The Korean Society For Biomedical Laboratory Sciences.

Ju-Hyung Park1, Jong-Gab Jun2 and Jin-Kyung Kim1,

1Department of Biomedical Science, Catholic University of Daegu, Gyeongsan-Si 712-702, Korea,
2Department of Chemistry and Institute of Natural Medicine, Hallym University, Chuncheon 200-702, Korea
Correspondence to: Jin-Kyung Kim
Department of Biomedical Science, College of Natural Science, Catholic University of Daegu, 13-13 Hayang-Ro, Gyeongsan-Si, Gyeongsangbuk-Do 712-702, Korea.
Tel: +82-53-850-3774, Fax: +82-53-850-3774
Received March 25, 2014; Revised May 14, 2014; Accepted May 24, 2014.
Previous our study demonstrated that ailanthoidol (3-deformylated 2-arylbenzo[b]furan), a neolignan from Zanthoxylum ailanthoides or Salvia miltiorrhiza Bunge, is a novel anti-inflammatory agent. In this investigation, we examined the anti-adipogenic effect of ailanthoidol. Our data showed that ailanthoidol suppressed lipid droplet formation and adipocyte differentiation in 3T3-L1 cells. Treatment of the 3T3-L1 adipocytes with ailanthoidol resulted in an attenuation of the releases of leptin and interleukin-6. The expression of peroxisome proliferator-activated receptor (PPAR)款 and CCAAT/ enhancer-binding protein (C/EBP)慣, the central transcriptional regulators of adipogenesis, was decreased by treatment with ailanthoidol. Additionally, ailanthoidol treatment increased the phosphorylation levels of 5' adenosine monophosphateactivated protein kinase. These results suggest that ailanthoidol effectively suppresses adipogenesis and that it exerts its role mainly through the significant down-regulation of PPAR款 and C/EBP慣 expression. Our findings provide important insights into the mechanisms underlying the anti-adipogenic activity of ailanthoidol.
Keywords : Adipogenesis, Ailanthoidol, C/EBPalpha, PPARgamma