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Efficacy of Disinfectants against Health-Associated Multi-drug Resistant Clinical Isolates
Biomed Sci Letters 2021;27:264-269
Published online December 31, 2021;  https://doi.org/10.15616/BSL.2021.27.4.264
© 2021 The Korean Society For Biomedical Laboratory Sciences.

Jeong-Lib An1,* , Sang-Ha Kim2,* , Young-Bin Yu3,* , Sunghyun Kim4,* , Moo-Sik Lee5,†,* and Young-Kwon Kim6,†,*

1Department of Health Sciences, The Graduate School of Konyang University, Daejeon 35365, Korea
2Department of Laboratory Medicine, Konyang University Hospital, Daejeon 35365, Korea
3Department of Biomedical Laboratory Science, College of Medical Sciences, Konyang University, Daejeon 35365, Korea
4Department of Clinical Laboratory Sciences, College of Health Sciences, Catholic University of Pusan, Busan 46252, Korea
5Department of Medicine, College of Medicine, Konyang University, Daejeon 35365, Korea
6Department of Health Sciences, The Graduate School of Konyang University, Daejeon 35365, Korea
Correspondence to: *Professor.
Corresponding author: Moo-Sik Lee. Department of Medicine, College of Medicine, Konyang University, 158 Gwanjeodong-ro, Seo-gu, Daejeon 35365, Korea.
Tel: 82-10-2514-4527, Fax: 82-42-600-8631, e-mail: mslee@konyang.ac.kr
Corresponding author: Young-Kwon Kim. Department of Health Sciences, The Graduate School of Konyang University, 158 Gwanjeodong-ro, Seo-gu, Daejeon 35365, Korea.
Tel: 82-10-6436-3245, Fax: 82-42-600-6401, e-mail: ykkim3245@konyang.ac.kr
Received September 27, 2021; Revised November 15, 2021; Accepted November 16, 2021.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
 Abstract
The purpose of this study was to evaluate the disinfecting efficacy of peracetic acid (PAA), sodium hypochlorite (NaOCl) and phenol, which are representative disinfectants in medical environments using four types of multi-drug resistance (MDR) clinical isolates with healthcare-associated infections (HAI). 26 antibiotic susceptibility tests were conducted for the four types of MDR clinical isolates in the same way as for clinical specimens. The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of the disinfectants were determined by using in vitro liquid medium dilution method and inoculation of the plate medium. Both the MIC and MBC of phenol against MRSA and VRE were 3.1%, while those against KPC and MRPA were 6.2%. The MIC and MBC of peracetic acid (PAA) against MRSA, VRE, KPC, and MRPA were 0.18%. The MIC and MBC of sodium hypochlorite (NaOCl) against MRSA were 0.39% and 0.78%, respectively. Both values of MIC and MBC were 0.78% for VRE. In addition, KPC and MRPA showed 0.39% for MIC and 0.78% for MBC. For all MDR strains used in this study, sodium hypochlorite and peracetic acid showed significant sterilizing efficiency, while no clear correlation was identified between antibiotic resistance clinical isolated and ability of disinfection.
Keywords : Minimum Inhibition Concentration (MIC), Multi-drug Resistance clinical isolates, Peracetic acid (PAA), Phenol, Sodium hypochlorite
꽌 濡

理쒓렐 뱾뼱 肄붾줈굹諛붿씠윭뒪-19 媛먯뿼利(Corona Virus Disease 19, COVID-19)쓽 꽭怨꾩쟻 쑀뻾, 以묒쬆湲됱꽦 샇씉湲곌컧뿼利 諛붿씠윭뒪 媛먯뿼利(severe acute respiratory syndrome coronavirus, SARS-CoV), 以묐룞 샇씉湲곌컧뿼利 諛붿씠윭뒪 媛먯뿼利(middle east respiratory syndrome coronavirus, MERS-CoV) 떊醫낆씤뵆猷⑥뿏옄 媛먯뿼利(Influenzavirus H1N1), 뿉蹂쇰씪諛붿씠윭뒪 媛먯뿼利(Ebola virus disease), 吏移대컮씠윭뒪 媛먯뿼利(Zikavirus disease), 쓽猷뚭젴 媛먯뿼 궡꽦洹 媛먯뿼(Healthcare-Associated Multi-drug Resistance Bacteria) 벑 꽭怨 룄泥섏뿉꽌 떎뼇븳 깉濡쒖슫 蹂묒썝泥대줈 씤븳 떊醫 異쒗쁽 媛먯뿼蹂(Emerging infectious disease)쓽 利앷 媛먯뿼寃쎈줈쓽 떎뼇솕濡 씤빐 吏덈퀝쓽 뼇긽룄 떎뼇븯寃 蹂솕븯怨 엳뼱 媛먯뿼蹂묒쓽 삁諛⑷낵 뵾빐 솗궛쓣 諛⑹븯젮뒗 끂젰씠 利앷븯怨 엳떎(Chung, 2018).

듅엳 쓽猷뚭젴 媛먯뿼쓣 씪쑝궎뒗 誘몄깮臾쇰뱾 二쇰줈 吏곴컙젒쟻씤 젒珥됱쑝濡 씤빐 怨듦린굹 鍮꾨쭚쓣 넻빐꽌 쟾뙆릺湲곕룄 븯硫, 삤뿼맂 臾쇱씠굹 떇뭹, 닾빟 諛 쓽猷 泥섏튂 諛 쓽猷 솚寃 벑쓣 넻빐꽌 쟾뙆릺뒗 寃쎌슦룄 엳떎. 씪遺 솚옄뿉꽌 利앹긽쓣 룞諛섑븯뒗 媛먯뿼씠 諛쒖깮븯뒗 寃쎌슦룄 엳쑝굹, 遺遺 솚옄뿉꽌뒗 利앹긽 뾾씠 꽭洹좎쓣 蹂댁쑀븯怨 엳뒗 吏묐씫솕 긽깭濡 굹굹湲곕룄 븳떎. 洹몃윭굹 솚옄媛 臾댁쬆긽 蹂닿퇏옄씤 寃쎌슦뿉룄 二쇰 솚寃쎌쓣 삤뿼떆궗 닔 엳湲 븣臾몄뿉 臾댁쬆긽 蹂닿퇏옄룄 빆깮젣궡꽦 꽭洹좎쓽 쟾뙆뿉 以묒슂븳 슂씤쑝濡 옉슜븯硫, 쓽猷뚭젴 媛먯뿼 쟾 꽭怨꾩쟻쑝濡 씠솚쑉怨 궗留앸쪧쓽 以묒슂븳 썝씤씠 맂떎(Guimarães et al., 2000).

쓽猷 솚寃쎌쓽 냼룆諛⑸쾿 蹂묒썝꽦 삉뒗 옞옱쟻 蹂묒썝꽦 誘몄깮臾쇰줈 씤븳 媛먯뿼利앹쓣 삁諛⑺븯嫄곕굹 媛먯냼떆궎뒗 寃껋쓣 紐⑺몴濡 븳떎. HAIs (healthcare-associated infections) 쟾 꽭怨꾩쟻쑝濡 씠솚쑉怨 궗留앸쪧쓽 以묒슂븳 썝씤쓣 굹궡湲 븣臾몄뿉 쓽猷 솚寃 몴硫댁쓽 삤뿼 怨듭쨷蹂닿굔븰쟻쑝濡 留ㅼ슦 以묒슂븳 臾몄젣媛 릺怨 엳떎(Quinn et al., 2015).

궡洹좎냼룆젣쓽 궡洹좏슚怨쇰뒗 냼룆젣媛 誘몄깮臾쇨낵 젒珥됲븯뒗 떆媛꾧낵 냼룆젣쓽 냽룄, 궡洹좎냼룆젣瑜 궗슜븷 븣쓽 삩룄쓽 蹂솕 pH, 쑀湲곕Ъ吏덉쓽 議댁옱 뿬遺, 誘몄깮臾쇱쓽 醫낅쪟 벑뿉 쓽빐 쁺뼢쓣 諛쏅뒗떎. 뵲씪꽌 쓽猷뚭린愿 諛 怨듦났옣냼뿉꽌쓽 솚寃 듅꽦쓣 怨좊젮븳 슚怨쇱쟻씤 궡洹좎냼룆젣瑜 꽑깮븯뿬 궗슜븯뿬빞 誘몄깮臾 利앹떇怨 媛먯뿼諛쒖깮쓣 삁諛⑺븷 닔 엳湲 븣臾몄뿉 쓽猷뚯떆꽕뿉꽌 쟻슜븷 냼룆젣쓽 떆뿕愿 궡(in vitro) 빆洹 솢꽦쓣 議곗궗븯뒗 寃껋 냼룆젣 냼룆諛⑸쾿 꽑깮뿉 以묒슂븳 쓽誘멸 엳떎(Lee et al., 2005).

蹂 떎뿕뿉 궗슜븳 냼룆젣 以 븯굹씤 怨쇱큹궛(Peracetic acid, PAA), C2H4O3) 臾쇱뿉 끃븘엳뒗 珥덉궛(CH3COOH)怨 怨쇱궛솕닔냼(H2O2)쓽 샎빀臾쇰줈 솚寃 몴硫 궡洹좎젣濡 옉슜븯硫 떎瑜 궛솕젣 쑀궗븯寃 옉슜븯뿬 꽭룷踰 닾怨쇨낵젙쓣 뙆愿댄븯怨 떒諛깆쭏, 슚냼 諛 湲고 궗궛臾쇱쓽 솴솕寃고빀쓣 궛솕떆耳 궗硫명븳떎(Malchesky, 1993).

李⑥븘뿼냼궛굹듃瑜(Sodium hypochlorite, NaOCl) 誘몄깮臾쇱쓽 떒諛깆쭏 꽭룷솢꽦쓣 뙆愿댄븯硫, 돱겢젅삤떚뱶 뿼湲곗쓽 뿼냼솕 쑀룄泥댁쓽 삎꽦쓣 넻빐 誘몄깮臾쇱쓽 DNA뿉 쑀빐븳 쁺뼢쓣 誘몄튂湲 븣臾몄뿉 꽭洹, 諛붿씠윭뒪 諛 怨고뙜씠쓽 냼룆뿉 슚怨쇱쟻씠硫, 寃쎌젣쟻쑝濡쒕룄 졃븯뿬 닔吏덉젙솕뿉 슚怨쇱쟻쑝濡 궗슜맆 닔 엳뒗 솕빀臾쇱씠떎. 議 냼룆젣濡 떎瑜 냼룆젣쓽 슚젰쓣 鍮꾧탳븷 븣쓽 湲곗쑝濡 릺怨 엳뒗 럹(phenol)쓣 궗슜븯떎(Maria et al., 2018).

쓽猷뚭젴 媛먯뿼 엫긽 遺꾨━洹좎<뿉 븳 빆깮젣 궡꽦뿉 빐꽌뒗 愿묐쾾쐞븯寃 뿰援щ릺怨 엳吏留, 쓽猷뚭젴 媛먯뿼 떎젣 궡꽦洹좎뿉 븳 냼룆옉슜怨쇱쓽 뿰愿꽦뿉 愿븳 뿰援щ뒗 냼닔뿉 遺덇낵븯硫, 援궡뿉꽌쓽 뿰援щ뒗 嫄곗쓽 뾾뒗 떎젙씠떎.

뵲씪꽌 蹂 뿰援ъ뿉꽌뒗 엫긽쓽 솚옄뿉꽌 쓽猷뚭젴 媛먯뿼 떎젣 궡꽦洹 4醫 MRSA, VRE, MRPA, KPC瑜 긽쑝濡 蹂묒썝 쓽猷뚰솚寃 냼룆젣濡 궗슜븯怨 엳뒗 몴쟻씤 냼룆젣씤 怨쇱큹궛(peracetic acid, PAA), 李⑥븘뿼냼궛 굹듃瑜(sodium hypochlorite, NaOCl), 諛 럹(phenol)뿉 븳 냼룆젰쓣 룊媛븯뿬 쓽猷 솚寃쎌쓽 媛먯뿼愿由щ 쐞븳 냼룆젣쓽 꽑깮뿉 李멸퀬븷 湲곗큹 옄猷뚮 젣怨듯븯怨좎옄 븯떎.

옱猷 諛 諛⑸쾿

꽭洹좎쓽 遺꾨━ 諛 룞젙

쟾 떆궡 K 븰蹂묒썝쓽 엫긽寃泥댁뿉꽌 遺꾨━맂 꽭洹 以 Gram 뿼깋怨 1李 諛곗뼇쓣 떆뻾븯뿬 Gram 뼇꽦꽭洹 諛 Gram 쓬꽦꽭洹좎쓣 遺꾨━븯怨, VITEK Ⅱ compact (Biomerieux Durham NC, USA) 옄룞솕 룞젙 떆뒪뀥쑝濡 룞젙븯쑝硫, 쓽猷뚭젴 媛먯뿼 빆깮젣 궡꽦꽭洹 4醫낆쓣 遺꾨━븯떎.

떎뿕뿉 궗슜븳 냼룆젣

떎뿕뿉 궗슜븳 냼룆젣뒗 떆以묒뿉 뙋留ㅻ릺뒗 슜븸쓽 썝븸쓣 젣議곗뾽泥댁쓽 吏移⑥뿉 뵲씪 씗꽍븯뿬 궗슜븯떎.

1) 李⑥븘뿼냼궛굹듃瑜(Sodium hypochlorite), 6~14% available chlorine, SAMCHUN PURE CHEMICAL CO. LTD. Korea

2) 怨쇱큹궛(Peraetic acid), 1.5% solution trade name Peragreen Nexchem, CO, Korea

3) 럹(Phenol), A, >98.0% trade name Bisphenol SAMCHUN PURE CHEMICAL CO. LTD. Korea

諛곗 諛 떆빟

怨좎븬 硫멸퇏맂 trypticase soy broth (TSB, Oxoid, UK), trypticase soy agar (TSA, Oxoid, UK) 삁븸슦臾대같吏(sheep blood agar plate, Oxoid, UK)瑜 궗슜븯떎. 떆뿕愿(25×150 mm), 빆삩닔議(56℃ JeioTech CO, Korea), 빆삩湲(37℃ JeioTech CO, Korea), 諛곗뼇湲(25~30℃ JeioTech CO, Korea), 럹듃由щ뵒돩, 쁽誘멸꼍, 깮由ъ떇뿼닔 벑쓣 궗슜븯떎.

냼룆젣쓽 理쒖냼諛쒖쑁뼲젣냽룄(minimum inhibitory concentration, MIC) 痢≪젙

빟젣쓽 誘몄깮臾 諛쒖쑁쓣 뼲젣븯뒗 理쒖냼냽룄瑜 痢≪젙븯湲 쐞빐 떆뿕愿씗꽍踰뺤쓣 궗슜븯떎. 냼룆젣쓽 냽룄媛 1/2諛 씗꽍怨꾩뿴씠 릺룄濡 젣옉븳 TSB 諛곗뿉 떆뿕洹좎쓣 1 mL 뵫 젒醫낇븳 썑 씠瑜 37℃ 諛곗뼇湲곗뿉꽌 24떆媛 諛곗뼇븯뿬 젒醫낇븳 떆뿕 꽭洹좎쓽 諛쒖쑁 쑀臾대 쑁븞쑝濡 솗씤븯뿬 理쒖냼諛쒖쑁뼲젣냽룄瑜 뙋젙븯떎.

냼룆젣쓽 理쒖냼궡洹좊냽룄(minimum bacteriocidal concentration, MBC) 痢≪젙

理쒖냼諛쒖쑁뼲젣냽룄 떆뿕愿쓣 룷븿븳 쑁븞쑝濡 꽭洹좎쓽 利앹떇씠 蹂댁씠吏 븡뒗 떆뿕愿뿉꽌 硫멸퇏맂 諛깃툑씠濡 1 loop瑜 뵲꽌 삁븸슦臾대같吏뿉 젒醫낇븳 썑 37℃ 諛곗뼇湲곗뿉꽌 24떆媛 諛곗뼇 썑 꽭洹좎쓽 諛쒖쑁 뿬遺瑜 愿李고븯떎.

寃 怨

쓽猷뚭젴媛먯뿼 빆깮젣 궡꽦 꽭洹좎쓽 遺꾨━

엫긽寃泥댁뿉꽌 遺꾨━맂 꽭洹 以 硫뷀떚떎由 궡꽦 솴깋룷룄븣洹(Methicillin-resistant Staphylococcus aureus, MRSA), 諛섏퐫留덉씠떊 궡꽦 옣븣洹(vancomycin-resistant Enterococcus, VRE), 移대컮럹꽩 遺꾪빐슚냼 깮꽦 룓졃 留됰洹(Klebsiella pneumoniae Carbapenemase Producing, KPC), 떎젣 궡꽦 끃냽洹(multidrug-resistant Pseudomonas aeruginosa, MRPA) 벑 쓽猷뚭젴 媛먯뿼꽭洹 4醫낆쓣 遺꾨━븯떎.

냼룆젣쓽 理쒖냼諛쒖쑁뼲젣냽룄(minimum inhibitory concentration, MIC) 痢≪젙

쓽猷뚭젴 떎젣궡꽦 꽭洹좎뿉 븳 냼룆젣쓽 냽룄瑜 100%, 50%, 25%, 12.5%, 6.25%, 3.13%, 1.56%, 0.78%, 0.39%, 0.18%濡 怨꾩뿴 씗꽍븯뿬 37℃ 諛곗뼇湲곗뿉꽌 24떆媛 諛곗뼇 썑 쑁븞쑝濡 愿李고븳 寃곌낵 샎긽쓣 蹂 닔 뾾뒗 媛옣 궙 떆뿕愿쓽 냽룄瑜 理쒖냼諛쒖쑁뼲젣냽룄(MIC)濡 븯뿬 냼룆젣 냽룄瑜 솗씤븳 寃곌낵 議 洹좎<씤 Escherichia coli ATCC 25922 Methicillin resistant Staphylococcus aureus (MRSA)뒗 Peracetic acid Sod. hypochlorite쓽 0.18% 냽룄쓽 떆뿕愿源뚯 利앹떇씠 뾾뿀쑝굹, Phenol뿉꽌뒗 1.6% 씠븯쓽 냽룄뿉꽌 꽭洹좎씠 利앹떇븯怨, 3.1% 씠긽쓽 냽룄뿉꽌뒗 紐⑤몢 꽭洹좎씠 利앹떇븯吏 븡븘꽌 Phenol쓽 MIC뒗 3.1%濡 뙋젙븯떎. Vancomycin resistant Enterococcus faecium (VRE) Peracetic acid뿉꽌뒗 紐⑤뱺 냽룄쓽 슜븸뿉꽌 利앹떇븯吏 븡븯쑝굹 Sod. hypochlorite 슜븸뿉꽌뒗 0.8% 슜븸 씠븯쓽 냽룄뿉꽌 紐⑤몢 利앹떇븯쑝硫, 1.6% 씠긽쓽 냽룄뿉꽌遺꽣 紐⑤몢 利앹떇븯吏 븡븘 1.6% 냽룄瑜 Sod. hypochlorite 슜븸쓽 MIC濡 뙋젙븯떎. Phenol 슜븸뿉꽌뒗 1.6% 슜븸 씠븯쓽 냽룄뿉꽌 紐⑤몢 利앹떇븯쑝硫, 3.2% 씠긽쓽 냽룄뿉꽌遺꽣뒗 紐⑤몢 利앹떇븯吏 븡븘 3.2% 냽룄瑜 Phenol 슜븸쓽 MIC濡 뙋젙븯떎. Carbapenemase Producing Klebsiella pneumoniae (KPC)怨 Multi-drug resistance Pseudomonas aeruginosa (MRPA)뿉꽌뒗 Peracetic acid Sod. Hypochlorite 슜븸뿉꽌뒗 紐⑤뱺 냽룄뿉꽌 利앹떇릺吏 븡븯吏留 Phenol 슜븸뿉꽌뒗 3.1% 씠븯쓽 냽룄뿉꽌 紐⑤몢 利앹떇븯쑝硫, 6.2% 씠긽쓽 냽룄뿉꽌遺꽣뒗 紐⑤몢 利앹떇븯吏 븡븘 6.2% 냽룄瑜 Phenol 슜븸쓽 MIC濡 뙋젙븯떎(Table 1).

Minimum inhibitory concentration test of disinfectant for healthcare-associated multi-drug resistant from clinical isolates

Disinfectant (%) Escherichia coli ATCC 25922 MRSA VRE KPC MRPA
6.2 3.1 1.6 0.8 6.2 3.1 1.6 0.8 6.2 3.1 1.6 0.8 6.2 3.1 1.6 0.8 6.2 3.1 1.6 0.8
Peracetic acid NG NG NG NG NG NG NG NG NG NG NG NG NG NG NG NG NG NG NG NG
Phenol NG NG G G NG NG G G NG NG G G NG G G G NG G G G
Sod. hypochlorite NG NG NG NG NG NG NG NG NG NG NG G NG NG NG NG NG NG NG NG

Abbreviation; NG: no growth, G; growth, MRSA; Methicillin resistant Staphylococcus aureus, VRE; Vancomycin resistant Enterococcus faecium, KPC; Klebsiella pneumoniae Carbapenemase Producing, MRPA; Multi-drug resistance Pseudomonas aeruginos



냼룆젣쓽 理쒖냼궡洹좊냽룄(minimum bactericidal concentration, MBC) 痢≪젙

쓽猷뚭젴 떎젣궡꽦 꽭洹좎뿉 븳 理쒖냼諛쒖쑁뼲젣냽룄(MIC)瑜 痢≪젙븳 썑 MIC 씠긽쓽 꽭洹좎씠 利앹떇븯吏 븡 냽룄쓽 떆뿕愿쓣 긽쑝濡 삁븸슦臾대같吏뿉 젒醫낇븯뿬 빆깮젣媛 뱾뼱 엳吏 븡 諛곗뿉꽌 옄 꽭洹좎쓽 吏묐씫 닔 鍮 99.99% 씠긽쓽 궡洹좏슚怨쇰 굹궡뒗 냽룄瑜 MBC濡 젙븯뿬 냼룆젣 냽룄瑜 솗씤븳 寃곌낵 議 洹좎<씤 Escherichia coli ATCC 25922뒗 Peracetic acid뿉꽌 MIC MBC媛 0.18% 냽룄濡 媛숈 냽룄瑜 蹂댁쑝硫, Sod. hypochlorite뿉꽌뒗 MIC뒗 0.39%瑜 蹂댁怨, Phenol뿉꽌 MIC MBC뒗 紐⑤몢 3.1%瑜 蹂댁떎. Methicillin resistant Staphylococcus aureus (MRSA)뒗 Peracetic acid뿉꽌 MIC MBC媛 0.18% 냽룄濡 媛숈 냽룄瑜 蹂댁怨, Sod. hypochlorite뿉꽌뒗 MIC뒗 0.39%瑜 蹂댁怨, MBC뒗 0.78%瑜 蹂댁쑝硫, Phenol뿉꽌 MIC MBC뒗 紐⑤몢 3.1%瑜 蹂댁떎.

Vancomycin resistant Enterococcus faecium (VRE) Peracetic acid뿉꽌뒗 MIC MBC媛 0.18% 냽룄濡 媛숈 냽룄瑜 蹂댁쑝硫, Sod. hypochlorite뿉꽌뒗 MIC MBC뒗 紐⑤몢 0.78%瑜 蹂댁떎.

Phenol뿉꽌 MIC MBC뒗 紐⑤몢뿉꽌 3.1%瑜 蹂댁떎. Carbapenemase Producing Klebsiella pneumoniae (KPC) Multi-drug resistance Pseudomonas aeruginosa (MRPA)뿉꽌뒗 紐⑤몢 Peracetic acid뿉꽌뒗 MIC MBC媛 0.18% 냽룄濡 媛숈 냽룄瑜 蹂댁쑝硫, Sod. hypochlorite뿉꽌뒗 MIC뒗 0.39%, MBC뒗 0.78%瑜 蹂댁떎. Phenol뿉꽌 MIC MBC뒗 紐⑤몢 6.2%瑜 蹂댁떎(Table 2).

Minimum inhibitory concentration and minimum bactericidal concentration of disinfectant for healthcare-associated multi-drug resistant from clinical isolates

Microorganisms Peracetic acid (%) Phenol (%) Sodium hypochlorite (%)
MIC MBC MIC MBC MIC MBC
Escherichia coli ATCC 25922 Control 0.18 0.18 3.1 3.1 0.18 0.36
Methicillin resistant Staphylococcus aureus (MRSA) 0.18 0.18 3.1 3.1 0.39 0.78
Vancomycin resistant Enterococcus faecium (VRE) 0.18 0.18 3.1 3.1 0.78 0.78
Klebsiella pneumoniae Carbapenemase Producing (KPC) 0.18 0.18 6.2 6.2 0.39 0.78
Multi-drug resistant Pseudomonas aeruginosa (MRPA) 0.18 0.18 6.2 6.2 0.39 0.78

Abbreviation; MIC; minimum inhibitory concentration

MBC; minimum bactericidal concentration


怨 李

쓽猷뚰솚寃쎌뿉꽌쓽 냼룆 蹂묒썝꽦 삉뒗 옞옱쟻 蹂묒썝꽦 誘몄깮臾쇰줈 씤븳 媛먯뿼利앹쓣 삁諛⑺븯嫄곕굹 媛먯냼떆궎뒗 寃껋쓣 紐⑺몴濡 븳떎. 쓽猷뚰솚寃 몴硫댁쓽 삤뿼 怨듭쨷蹂닿굔븰쟻쑝濡 留ㅼ슦 以묒슂븳 臾몄젣媛 릺怨 엳떎(Quinn et al., 2015). 듅엳 쓽猷뚭젴 媛먯뿼 떎젣 궡꽦洹좊뱾 蹂묒썝쓽 꽭硫닿린援, 솕옣떎, 泥냼룄援, 湲덉냽 뙆씠봽 諛 諛붾떏 諛곗닔援 벑뿉꽌 옄씪硫 遺덈━븳 議곌굔쑝濡쒕꽣 洹좎껜瑜 蹂댄샇븯湲 쐞빐 깮臾쇰쭑쓣 留뚮뱾 닔 엳떎. 떎뼇븳 빆洹좊Ъ吏덉뿉 븳 궡꽦 솚옄쓽 移섎즺 諛⑸쾿쓣 蹂솕떆궗 닔 엳쑝硫, 쓽猷뚰솚寃쎌뿉꽌 냼룆諛⑸쾿쓣 媛쒖꽑븷 븘슂꽦씠 利앷븯怨 엳떎(Al-Taw?q and Tambyah, 2014).

뵲씪꽌 怨쇱큹궛 씠 媛숈 떒젏씠 뾾湲 븣臾몄뿉 쓽猷뚯옣鍮꾩쓽 꽭泥숈뿉 궗슜릺湲 떆옉븯쑝硫, FDA뒗 Steris 20 Sterilant瑜 궡떆寃, 愿젅寃 諛 湲고 쇅怨쇱슜 湲곌뎄瑜 쐞븳 옄룞솕맂 븸泥 솕븰 궡洹좎쿂由 옣移섏씤 Steris System 1怨 븿猿 궗슜븯룄濡 듅씤븯떎. 듅엳 쓽猷뚭린湲, 궡떆寃 諛 삁븸 닾꽍湲곗쓽 냼룆젣濡 怨쇨굅뿉 궗슜릺뜕 쟾넻쟻씤 냼룆젣瑜 泥댄븯뿬 궗슜븯怨 엳떎. 李⑥븘뿼냼궛굹듃瑜⑥ 媛뺣젰븳 궛솕젣씠硫 씤솕꽦 臾쇱쭏 諛 씤뜒꽣 諛섏쓳븯吏留 李⑥븘뿼냼궛굹듃瑜 슜븸 씤솕꽦씠 빟븳 뿼湲곗씠떎. 李⑥븘뿼냼궛 굹듃瑜⑥ 슫諛섍낵 蹂닿뿉 븞쟾븯硫, 냼룆 슚怨쇰뒗 뿼냼 媛뒪留뚰겮 슚怨쇱쟻씠떎. 씪諛섏쟻쑝濡 3~5% 닔슜븸쓣 냼룆젣濡 궗슜븯吏留 븘룷(spore)瑜 媛吏뒗 꽭洹좎뿉뒗 궡洹좊젰쓣 媛吏吏 紐삵븯뒗 븳怨꾧 엳떎(Maria et al., 2018).

씠 뿰援ъ뿉 궗슜븳 냼룆젣 以 Phenol뿉 븳 냼룆젰쓣 痢≪젙븳 寃곌낵 Methicillin resistant Staphylococcus aureus (MRSA) Vancomycin resistant Enterococcus faecium (VRE) 1.6% 씠븯쓽 냽룄뿉꽌 꽭洹좎씠 利앹떇븯怨, 3.1% 씠긽쓽 냽룄뿉꽌뒗 紐⑤몢 꽭洹좎씠 利앹떇븯吏 븡븘꽌 MIC MBC뒗 紐⑤몢 3.1%瑜 蹂댁쑝硫, Klebsiella pneumoniae Carbapenemase Producing (KPC)怨 Multi-drug resistance Pseudomonas aeruginosa (MRPA)뿉꽌뒗 3.1% 씠븯쓽 냽룄뿉꽌 紐⑤몢 利앹떇븯怨, 6.2% 씠긽쓽 냽룄뿉꽌遺꽣뒗 紐⑤몢 利앹떇븯吏 븡븘 MIC MBC뒗 紐⑤몢 6.2%瑜 蹂댁떎. 씠뒗 1:100쑝濡 씗꽍븳 phenol 슜븸뿉꽌 떎뿕뿉 궗슜븳 Staphylococcus aureus 20媛 洹좎< 紐⑤몢 궗硫명뻽吏留 Pseudomonas aeruginosa쓽 寃쎌슦 20媛 洹좎< 以 3洹좎<媛 깮議댄븯뿬 洹몃엺쓬꽦꽭洹좎뿉 phenol쓽 솢꽦씠 遺議깊븯떎뒗 寃곌낵 鍮꾩듂븳 寃곌낵瑜 굹깉떎(Maria et al., 2018). 씠踰 떎뿕뿉 궗슜븳 럹 씪諛섏쟻씤 꽭洹좎뿉 븳 궡洹 諛 寃고빑洹좎뿉 솢꽦씠 넂 寃껋쑝濡 븣젮졇 엳쑝硫, 怨좊냽룄뿉꽌 꽭룷踰쎌쓣 넻怨쇳븯뿬 꽭룷吏덉뿉 룆옉슜쓣 씪쑝耳 꽭룷 떒諛깆쭏쓣 移⑥쟾떆궎뒗 옉슜쓣 븯怨, 냽룄 諛 怨 遺꾩옄웾 럹 쑀룄泥대뒗 븘닔 슚냼 떆뒪뀥쓽 遺덊솢꽦솕 諛 꽭룷踰쎌뿉꽌 븘닔 궗臾쇱쭏쓣 늻異쒗븯뿬 꽭洹좎쓽 궗硫몄쓣 쑀諛쒗븯뒗 寃껋쑝濡 븣젮議뚮떎(Goddard and McCue, 2001).

Peracetic acid (PAA)뿉 븳 냼룆젰쓣 痢≪젙븳 寃곌낵 MRSA VRE, KPC 諛 MRPA뿉꽌뒗 紐⑤뱺 냽룄뿉꽌 利앹떇릺吏 븡븘 MIC MBC뒗 紐⑤몢 0.18%瑜 蹂댁뿬 媛옣 媛뺥븳 궡洹좊젰쓣 蹂댁떎. 怨쇱큹궛 湲곕컲 냼룆젣뒗 떇뭹 궛뾽怨 臾 삉뒗 븯닔泥섎━ 쉶궗뿉꽌 궗슜릺硫, 뿴 媛먯쓳 쓽猷 옣鍮 諛 蹂묒썝뿉꽌 궗슜븯뒗 궡떆寃 벑 옣鍮꾩쓽 삤뿼 젣嫄 諛 硫멸퇏뿉룄 鍮좊Ⅴ寃 옉슜븯硫, 꽭洹, 怨고뙜씠, 諛붿씠윭뒪 諛 룷옄뿉 슚怨쇱쟻쑝濡 옉슜븯뒗 寃껋쑝濡 븣젮議뚮떎(Chino et al., 2017).

蹂 뿰援ъ뿉꽌 Sodium hypochlorite (NaOCl) 슜븸뿉꽌 MRSA쓽 MIC뒗 0.39%瑜 蹂댁怨, MBC뒗 0.78%瑜 蹂댁쑝硫, VRE뒗 MIC MBC뒗 紐⑤몢 0.78%瑜 蹂댁떎. KPC MRPA뒗 媛곴컖 MIC뒗 0.39%, MBC뒗 0.78%瑜 蹂댁떎. NaOCl 슜븸 옒 븣젮吏 빆洹좎옉슜怨 議곗쭅슜빐 뒫젰쑝濡 씤빐 70뀈 씠긽 궗슜릺뼱 솕吏留 0.5 % 궡吏 5.25% 踰붿쐞 궡쓽 理쒖쟻 냽룄뿉 븳 씪諛섏쟻씤 빀쓽뒗 議댁옱븯吏 븡뒗 寃껋쑝濡 븣젮졇 엳쑝硫, Siqueira et al.쓽 뿰援ъ뿉꽌뒗 移섏븘 洹쇨 떎뿕뿉꽌 0.5% 궡吏 5% 踰붿쐞쓽 NaOCl 냽룄 궗씠뿉 빆洹좏슚怨쇱쓽 쑀쓽븳 李⑥씠瑜 굹궡吏 븡븯떎뒗 蹂닿퀬룄 엳떎(Siqueira et al., 2000).

Berber et al.쓽 뿰援ъ뿉꽌뒗 移섏븘 꽭愿뿉 븳 떆뿕냽룄 궗씠뿉 넻怨꾩쟻쑝濡 쑀쓽븳 李⑥씠媛 엳뿀떎뒗 蹂닿퀬룄 엳뼱 蹂묒썝솚寃 냼룆뿉꽌 洹좎쥌蹂, 냽룄蹂, 뿬윭 媛吏 議곌굔뿉꽌 醫 뜑 떎뼇븳 뿰援ш 릺뼱빞 븷 寃껋쑝濡 깮媛곷맂떎(Berber et al., 2006).

떎뿕뿉 궗슜맂 紐⑤뱺 쓽猷뚭젴 떎젣궡꽦 洹좎<뿉 빐 李⑥븘뿼냼궛 굹듃瑜④낵 怨쇱큹궛 紐⑤몢 媛뺥븳 궡洹좊젰씠 엳뿀吏留 럹뿉 빐꽌뒗 洹몃엺뼇꽦꽭洹좎뿉꽌뒗 媛뺥븳 궡洹좊젰쓣 洹몃엺쓬꽦꽭洹좎뿉꽌 빟븳 궡洹좊젰쓣 媛吏怨 엳뼱 엫긽遺꾨━ 洹좎<뿉 븳 빆깮젣 궡꽦怨 냼룆젣쓽 궡洹좊젰 궗씠쓽 긽愿愿怨꾨 諛쒓껄븯吏 紐삵뻽떎. Rutala et al.쓽 뿰援ъ뿉꽌 엫긽遺꾨━ 洹좎<뿉 븳 빆깮젣뿉 븳 궡꽦怨 냼룆젣쓽 궡洹좊젰 궗씠쓽 紐낅갚븳 긽愿愿怨꾨 諛쒓껄븯吏 紐삵뻽떎뒗 蹂닿퀬 슦由ъ쓽 뿰援ъ뒗 씪移섎맂 寃곌낵瑜 蹂댁떎(Rutala et al., 1997).

쓽猷뚭젴 떎젣궡꽦 꽭洹 媛먯뿼씠 利앷븯怨, 쓽猷뚰솚寃쎌뿉꽌쓽 媛먯뿼愿由щ 쐞븳 냼룆怨 삤뿼젣嫄곕 쐞븳 냼룆젣쓽 꽑깮씠 뼱뒓 븣蹂대떎 以묒슂븳 긽솴뿉꽌 쓽猷뚭젴 媛먯뿼 떎젣궡꽦 꽭洹좎뿉 븳 냼룆젣쓽 슚뒫 떎뿕쓣 떆룄뻽떎뒗뜲 쓽誘멸 엳뒗 寃껋쑝濡 깮媛곷맂떎.

ACKNOWLEDGEMENT

None.

CONFLICT OF INTEREST

No potential conflict of interest relevant to this article was reported.

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