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The Effect of Angelica gigas Nakai on Immune Enhancement in Cyclophosphamide-induced Immune-suppressed Mice
Biomed Sci Letters 2022;28:120-126
Published online June 30, 2022;  https://doi.org/10.15616/BSL.2022.28.2.120
© 2022 The Korean Society For Biomedical Laboratory Sciences.

Byung-cheol Seo1,* and Su-Jin Kim2,†,* *

1Research Institute of Nutragen, Eumseong 27649, Korea
2Department of Biotechnology and Convergence, Daegu Haany University, Kyungsan 38578, Korea
Correspondence to: Su-Jin Kim. Department of Biotechnology and Convergence, Daegu Haany University, Kyungsan 38578, Korea.
Tel: +82-53-819-1389, Fax: +82-53-819-1389, e-mail: ksj1009@dhu.ac.kr
*Ph D, **Professor.
Received June 2, 2022; Revised June 25, 2022; Accepted June 28, 2022.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
 Abstract
Natural products are important sources for drug development because they have a wide variety of useful biological properties. Angelica gigas Nakai (AGN) has been used as an herbal medicine. The present study was designed to evaluate the immune-enhancing effect of AGN in the cyclophosphamide (CP) induced immune-suppressed mice. As the result, we found that CP induced the reductions of body ratio, spleen weights, hematopoietic parameter and cytokine productions in mice. However, AGN recovered immunosuppression-mediated decreased body ratio, spleen and thymus weights as well as regulation of hematopoietic parameter including white blood cell, lymphocyte, and neutrophil. According to histological study, AGN regenerated on CP-mediated injured spleen. Moreover, AGN increased the CP-induced reduction of cytokine expression in spleen tissue. Collectively, the findings provide experimental evidence that AGN may be a candidate for health-improving herbs.
Keywords : Angelica gigas Nakai, Immune-enhancement, Cyclophosphamide, Cytokine
꽌 濡

硫댁뿭諛섏쓳 쇅遺뿉꽌 쑀엯릺뒗 諛뺥뀒由ъ븘, 諛붿씠윭뒪 媛숈 떎뼇븳 媛먯뿼썝쑝濡쒕꽣 떊泥대 蹂댄샇븯湲 쐞븳 諛⑹뼱옉슜씠떎(Basha et al., 2014). 理쒓렐, 硫댁뿭 議곗젅 옣븷 吏덊솚씠 利앷븯뒗 異붿꽭濡 듅엳 媛먯뿼, 뒪듃젅뒪, 끂솕 벑쑝濡 씤븳 硫댁뿭떆뒪뀥 씠긽 븫, 쟾뿼蹂, 븣젅瑜닿린, 泥쒖떇 諛 愿젅뿼怨 媛숈 硫댁뿭愿젴 吏덊솚쓣 쑀諛쒖떆궎뒗 寃껋쑝濡 蹂닿퀬릺怨 엳떎(Kumar and Bhat, 2016). 硫댁뿭 泥닿퀎뒗 꽑泥쒖쟻 硫댁뿭怨 썑泥쒖쟻 硫댁뿭씠 쑀湲곗쟻씤 긽샇옉슜쑝濡 議곗젅맂떎. 꽑泥쒖쟻 硫댁뿭 媛먯뿼 珥덇린뿉 愿묐쾾쐞븳 蹂묒썝泥댁뿉 븳 鍮꾪듅씠쟻쑝濡 씪뼱굹硫, 썑泥쒖쟻 硫댁뿭 솢꽦솕맂 T 由쇳봽援, B 由쇳봽援ш 媛먯뿼뿉 빐 듅씠쟻쑝濡 씪뼱굹뒗 寃껋쑝濡 듅젙 媛먯뿼썝뿉 븳 湲곗뼲諛섏쓳씠 쑀룄릺뼱 吏덈퀝쓣 삁諛⑺븷 닔 엳떎(Nathaniel et al., 2020). B 由쇳봽援щ뒗 빆썝怨 寃고빀븯뒗 빆泥대 깮꽦븯硫댁꽌 硫댁뿭湲곕뒫쓣 쑀룄븯怨 T 由쇳봽援щ뒗 떎뼇븳 궗씠넗移댁씤쓣 遺꾨퉬븿쑝濡쒖뜥 硫댁뿭諛섏쓳쓣 議곗젅븳떎(Delves and Roitt, 2000; Hoffman et al., 2016). 硫댁뿭諛섏쓳 議곗젅뿉꽌 씤꽣猷⑦궓(IL)-2 T 由쇳봽援 遺꾪솕瑜 議곗젅븯怨, 씤꽣럹濡(INF)-γ뒗 떇꽭룷瑜 룷븿븳 硫댁뿭꽭룷 솢꽦솕뿉 愿뿬븳떎. 듅엳 IL-2 IFN-γ 寃고븤 떎뼇븳 蹂묒썝泥 븳 媛먯뿼씠 뜑 돺寃 諛쒖깮맂떎怨 蹂닿퀬릺뼱 엳떎(Dalton et al., 1993). 뵲씪꽌 궗씠넗移댁씤 솢꽦쓣 議곗젅븯뒗 泥쒖뿰臾쇱 硫댁뿭利앷컯 湲곕뒫꽦 냼옱濡쒖꽌 솢슜媛뒫꽦씠 엳쓣 寃껋쑝濡 궗猷뚮맂떎.

Cyclophosphamide (CP)뒗 DNA 븣궗젅씠뀡 쑀룄젣濡 諛깊삁蹂, 궃냼븫 諛 쑀諛⑹븫 벑 떎뼇븳 醫낆뼇쓽 移섎즺瑜 쐞빐 궗슜릺怨 엳떎(Huitema et al., 2000). 븫솚옄뱾뿉寃 愿묐쾾쐞븯寃 궗슜릺뒗 移섎즺젣엫뿉 遺덇뎄븯怨 CP 닾뿬뒗 泥댁쨷 媛먯냼, 삁냼뙋 媛먯냼利 諛 由쇳봽援 媛먯냼瑜 넻븳 硫댁뿭뼲젣 벑 떖媛곹븳 遺옉슜씠 蹂닿퀬릺怨 엳떎(De Souza et al., 2000). 뵲씪꽌 빆븫 移섎즺젣濡 씤븳 泥대궡硫댁뿭 湲곕뒫 븯瑜 셿솕떆궗 닔 엳뒗 硫댁뿭議곗젅 湲곕뒫꽦 냼옱 諛쒓뎬 뿰援ш 吏냽쟻쑝濡 슂援щ릺怨 엳뒗 떎젙씠떎.

李몃떦洹(Angelica gigas Nakai, AGN)뒗 誘몃굹由ш낵(Umbelliferae)뿉 냽븯뒗 떎뀈깮 珥덈낯쑝濡 빟꽦씠 뵲쑜븯怨, 臾대룆븯뿬 떎뼇븳 吏덊솚뿉 빟슜떇臾쇰줈 솢슜릺怨 엳떎. 듅엳, 李몃떦洹뒗 떦눊 媛쒖꽑 슚怨, 삁냼뙋 쓳吏묒뼲젣 옉슜, 빆궛솕 슚뒫 媛꾩쭏솚 媛쒖꽑 諛 빆븫옉슜 벑씠 蹂닿퀬릺怨 엳떎(Kim et al., 2004; Lee et al., 2009; Park et al., 2007). 李몃떦洹쓽 몴쟻씤 쑀슚꽦遺꾩쑝濡쒕뒗 decursin, nodakentin, limonene, β-sitosterol 諛 umbelliferon 벑씠 븣젮졇 엳떎(Kim et al., 2009). 理쒓렐 硫댁뿭湲곕뒫뿉 븳 以묒슂꽦씠 몢릺硫댁꽌 硫댁뿭湲곕뒫쓣 議곗젅븷 닔 엳뒗 湲곕뒫꽦 냼옱 媛쒕컻 뿰援ш 솢諛쒗엳 吏꾪뻾릺怨 엳쑝굹 슚뒫 諛 븞쟾꽦 엯利앹쓣 쐞븳 怨쇳븰쟻 洹쇨굅媛 遺議깊븳 떎젙씠떎. 蹂 뿰援ъ뿉꽌뒗 硫댁뿭利앷컯 湲곕뒫꽦 냼옱瑜 媛쒕컻븯湲 쐞빐 CP濡 쑀룄맂 硫댁뿭븯 룞臾쇰え뜽뿉꽌 李몃떦洹 異붿텧臾쇱쓽 硫댁뿭젰 利앷컯 솢꽦쓣 룊媛븯떎.

옱猷 諛 諛⑸쾿

떎뿕룞臾

蹂 떎뿕뿉꽌뒗 6二쇰졊쓽 썒꽦 ICR mice瑜 슚李(Daegu, Korea)뿉꽌 怨듦툒諛쏆븘 빟 7씪媛 쟻쓳떆궓 썑 옄쑀떇씠 臾쇱쓣 異⑸텇엳 怨듦툒빐二쇰㈃꽌 궗쑁븯떎. 궗쑁떎쓽 삩룄뒗 22±2℃濡 쑀吏릺뿀怨, 뒿룄뒗 50±5%濡 븯쑝硫, 議곕챸 12떆媛 二쇨린濡 궗슜븯떎. 蹂 룞臾쇱떎뿕 援ы븳쓽븰援 룞臾쇱떎뿕쑄由ъ쐞썝쉶 洹쒖젙뿉 뵲씪 吏꾪뻾븯떎.

떎뿕옱猷

CP, Eosin, hematoxylin 諛 洹 쇅 紐⑤뱺 떆빟 Sigma Chemical Co. (St. Louis, MO, USA)뿉꽌 援ъ엯븯떎. 留덉슦뒪 IL-2, IFN-γ 諛 TNF-α ELISA kit뒗 BD Biosciences (San Diego, CA, USA)뿉꽌 援ъ엯븯뿬 궗슜븯떎. Tissue Protein Extraction Reagent Intron Biotechnology (Seongnam, Korea)뿉꽌 bicinchoninic acid (BCA) assay kit뒗 Pierce Thermo Scientific (Rockford, IL, USA)뿉꽌 援ъ엯븯떎. 李몃떦洹 異붿텧臾쇱 (二)돱듃씪젨(Eumseong, Korea)뿉꽌 젣怨듬컺븘 궗슜븯떎.

硫댁뿭븯 紐⑤뜽 諛 떆猷 닾뿬

留덉슦뒪쓽 硫댁뿭븯瑜 쑀룄븯湲 쐞븯뿬 떆猷 닾뿬 떆옉 1씪 諛 3씪 쟾뿉 125 mg/kg쓽 CP瑜 2쉶 蹂듦컯뿉 二쇱궗븯怨 젙긽援곗뿉뒗 CP 떊 利앸쪟닔瑜 蹂듦컯 닾뿬븯떎. 硫댁뿭븯 쑀룄媛 醫낅즺맂 썑, 李몃떦洹 異붿텧臾쇱쓣 1씪 1쉶, 14씪 룞븞 寃쎄뎄닾뿬 븯떎. 珥 떎뿕援곗 硫댁뿭뼲젣瑜 쑀룄븯吏 븡 젙긽援(control), 硫댁뿭뼲젣 썑 利앸쪟닔 닾뿬援(CP), 硫댁뿭뼲젣 썑 李몃떦洹 異붿텧臾 10 mg/kg 닾뿬븳 떎뿕援(AGN10)怨 100 mg/kg쑝濡 닾뿬븳 떎뿕援(AGN100)쑝濡 珥 4洹몃9씠硫 援곕떦 7留덈━뵫 떎뿕뿉 궗슜븯떎.

泥댁쨷 痢≪젙

紐⑤뱺 떎뿕룞臾쇱쓽 泥댁쨷 CP 닾뿬濡 硫댁뿭븯 쑀룄 씠썑 利앸쪟닔 李몃떦洹 異붿텧臾쇱쓣 媛곴컖 닾뿬븯뒗 룞븞 씪젙떆媛꾩뿉 紐⑤뱺 떎뿕援곗쓽 媛쒖껜蹂 泥댁쨷쓣 痢≪젙븯떎.

옣湲 臾닿쾶 痢≪젙

理쒖쥌 씗깮씪뿉 紐⑤뱺 떎뿕援곗쓽 媛쒖껜蹂 鍮꾩옣 諛 쓨꽑쓣 쟻異쒗븯뿬 洹 以묐웾쓣 쟾옄슱쓣 씠슜븯뿬 g 떒쐞濡 媛곴컖 痢≪젙븯떎.

삁븸 遺꾩꽍

삁븸 EDTA tube뿉 떞븘 Roll mixer뿉 빟 30遺 쉶쟾떆궓 썑 삁븸遺꾩꽍湲곕 씠슜븯뿬 諛깊삁援(white blood cell), 由쇳봽援(lymphocyte) 諛 샇以묎뎄(neutrophill) 벑 삁븸븰쟻 寃궗瑜 吏꾪뻾븯떎.

鍮꾩옣 궡 cytokine 遺꾩꽍

理쒖쥌 씗깮씪뿉 鍮꾩옣 議곗쭅쓽 씪遺瑜 梨꾩랬븯뿬 꽭泥 썑 Tissue Protein Extraction Reagent쓽 lysis buffer瑜 궗슜븯뿬 鍮꾩옣 議곗쭅쓣 洹좎쭏솕 븯떎. 洹좎쭏솕맂 鍮꾩옣 議곗쭅 -4℃, 10,000 g쓽 議곌굔븯뿉꽌 5遺 룞븞 썝떖遺꾨━瑜 떎떆븯뿬 떒諛깆쭏씠 룷븿맂 긽痢듭븸쓣 痍⑦븯떎. BCA 떆빟쑝濡 떒諛깆쭏 젙웾 썑 ELISA kit瑜 솢슜븯뿬 IL-2, IFN-γ 諛 TNF-α쓽 닔移섎 痢≪젙븯떎.

議곗쭅븰쟻 遺꾩꽍

쟻異쒗븳 鍮꾩옣 議곗쭅 10% formalin뿉 怨좎젙떆궓 썑 깉닔 諛 뙆씪뿉 룷留ㅽ븯뿬 4 μm쓽 몢猿섎줈 젅렪쓣 젣옉븯떎. Hematoxylin-eosin (H&E)濡 뿼깋맂 議곗쭅 愿묓븰쁽誘멸꼍씤 LEICA DMi8 (LEICA, Germany)瑜 씠슜븯뿬 愿李고븯떎.

넻怨꾨텇꽍

떎뿕 寃곌낵뒗 理쒖냼 3踰 諛섎났 떎뿕븯怨 넻怨꾪봽濡쒓렇옩 Graph Pad Prism software 5.0瑜 씠슜븯뿬 룊洹(mean) ± 몴以렪李(standard deviation, S.D.)濡 굹궡뿀떎. 媛 뜲씠꽣쓽 넻怨꾨텇꽍 ANOVA Bonferroni's post-hoc test뿉 쓽빐 寃젙븯怨, P<0.05씤 寃쎌슦 쑀쓽꽦씠 엳뒗 寃껋쑝濡 뙋떒븯떎.

寃 怨

硫댁뿭븯 紐⑤뜽뿉꽌 泥댁쨷 蹂솕뿉 븳 李몃떦洹 異붿텧臾쇱쓽 쁺뼢

CP쓽 닾뿬濡 硫댁뿭븯媛 쑀룄릺硫 遺옉슜쑝濡 泥댁쨷 媛먯냼媛 蹂닿퀬릺뼱 엳떎. 蹂 뿰援ъ뿉꽌뒗 CP濡 쑀룄맂 硫댁뿭븯 紐⑤뜽뿉꽌 泥댁쨷 媛먯냼뿉 븳 李몃떦洹 異붿텧臾쇱쓽 쁺뼢쓣 痢≪젙븯떎. 떎뿕援곗쓽 泥댁쨷 蹂솕瑜 愿李고븳 寃곌낵, CP援곗뿉꽌뒗 젙긽援곌낵 鍮꾧탳빐꽌 泥댁쨷 蹂솕媛 媛먯냼븯怨, 李몃떦洹 異붿텧臾 닾뿬援곗뿉꽌뒗 CP濡 쑀룄맂 泥댁쨷 媛먯냼媛 셿솕릺뒗 寃껋쓣 솗씤븯떎. 떎뿕 醫낅즺 썑, 媛 떎뿕援 泥댁쨷 젙긽援곗쓽 寃쎌슦 23.5±0.8 g, CP援 21.8±0.9 g濡 CP 닾뿬濡 泥댁쨷씠 쑀쓽븯寃 媛먯냼맂 寃껋쑝濡 솗씤릺뿀떎. 諛섎㈃ CP 떒룆援곌낵 鍮꾧탳븯뿬 李몃떦洹 異붿텧臾 10 mg/kg 닾뿬援곗쓽 泥댁쨷 22.2±2.1 g, 李몃떦洹 異붿텧臾 100 mg/kg 닾뿬븳 떎뿕援곗 22.8±1.6 g쑝濡 CP濡 씤븳 泥댁쨷 媛먯냼媛 셿솕릺뒗 寃껋쑝濡 솗씤릺뿀떎(Fig. 1A). 媛 떎뿕援곗쓽 利앹껜웾쓣 痢≪젙븳 寃곌낵, 李몃떦洹 異붿텧臾쇨뎔 CP濡 쑀룄맂 硫댁뿭븯濡 媛먯냼맂 泥댁쨷씠 쉶蹂듬릺뒗 寃껋쓣 솗씤븷 닔 엳뿀떎(Fig. 1B).

Fig. 1. Effects of AGN on body weight in cyclophosphamide-induced immunosuppressed mice. (A) Body weight detected during oral treatment with AGN 10 mg/kg and 100 mg/kg for 14 days. (B) Body weight gain after test (Body weight at sacrifice-Body weight at start). Data were represented in the mean ± S.D. and results were analyzed by Tukey post hoc test (#P < 0.05 versus control, *P < 0.05 versus CP alone).

硫댁뿭븯 紐⑤뜽뿉꽌 옣湲 臾닿쾶 蹂솕뿉 븳 李몃떦洹 異붿텧臾쇱쓽 쁺뼢

CP 닾뿬뒗 꽭룷 룆꽦쑝濡 씤빐 鍮꾩옣怨 쓨꽑쓽 臾닿쾶媛 媛먯냼맂떎怨 蹂닿퀬릺뼱 엳떎. 蹂 뿰援ъ뿉꽌뒗 CP濡 쑀룄맂 硫댁뿭븯 紐⑤뜽뿉꽌 옣湲 臾닿쾶 蹂솕뿉 븳 李몃떦洹 異붿텧臾쇱쓽 쁺뼢쓣 痢≪젙븯떎. 떎뿕 寃곌낵, CP援곗뿉꽌뒗 鍮꾩옣쓽 以묐웾(0.077±0.02 g)씠 젙긽議곌뎔(0.139±0.018 g)뿉 鍮꾪빐꽌 쑀쓽븯寃 媛먯냼븯뒗 寃껋쑝濡 솗씤릺뿀떎. 諛섎㈃ 李몃떦洹 異붿텧臾쇱쓣 닾뿬븳 떎뿕援곗쓽 鍮꾩옣쓽 以묐웾 씠 CP濡 쑀룄맂 鍮꾩옣 臾닿쾶 媛먯냼媛 利앷맂 寃껋쑝濡 솗씤릺뿀떎. 듅엳 怨좊냽룄 李몃떦洹(100 mg/kg) 닾뿬援곗쓽 鍮꾩옣쓽 臾닿쾶뒗 0.12±0.021 g쑝濡 CP援(0.077±0.02 g)蹂대떎 쑀쓽븯寃 利앷맂 寃껋쑝濡 솗씤릺뿀떎(Fig. 2A). 쓨꽑쓽 以묐웾 蹂솕瑜 愿李고뻽쓣 븣, CP援곗쓽 以묐웾 0.045±0.004 g濡, 젙긽援곗쓽 以묐웾(0.0746±0.006 g)뿉 鍮꾪빐꽌 쑀쓽誘명븯寃 媛먯냼븯뒗 寃껋쓣 솗씤븯떎. 삉븳 怨좊냽룄쓽 李몃떦洹 異붿텧臾(100 mg/kg) 닾뿬援곗뿉꽌 쓨꽑쓽 以묐웾(0.058±0.004 g)씠 CP援곕낫떎 쑀쓽븯寃 利앷맂 寃껋쑝濡 솗씤릺뿀떎(Fig. 2B).

Fig. 2. Effects of AGN on organ weight in cyclophosphamide-induced immunosuppressed mice. Spleen (A) and thymus (B) weight after oral treatment with AGN 10 mg/kg and 100 mg/kg for 14 days. Data were represented in the mean ± S.D. and results were analyzed by Tukey post hoc test (#P < 0.05 versus control, *P < 0.05 versus CP alone).

硫댁뿭븯 紐⑤뜽뿉꽌 삁援ъ꽭룷뿉 誘몄튂뒗 李몃떦洹 異붿텧臾쇱쓽 슚怨

CP 닾뿬뒗 삁援ъ꽭룷 媛먯냼濡 硫댁뿭븯瑜 쑀룄븯뒗 寃껋쑝濡 蹂닿퀬릺뼱 엳떎(Son et al., 2003). CP濡 쑀룄맂 硫댁뿭븯 紐⑤뜽뿉꽌 李몃떦洹 異붿텧臾쇱쓽 삁븸꽭룷 蹂솕뿉 誘몄튂뒗 쁺뼢쓣 遺꾩꽍븯떎. 李몃떦洹 異붿텧臾쇱쓣 14씪媛 寃쎄뎄 닾뿬븳 썑 삁븸쓽 諛깊삁援 닔瑜 痢≪젙븳 寃곌낵, CP 닾뿬援곗쓽 諛깊삁援 닔뒗 젙긽援곗뿉 鍮꾪빐 媛먯냼릺뿀怨, 李몃떦洹 異붿텧臾 닾뿬援곗뿉꽌뒗 CP뿉 쓽빐 쑀룄맂 諛깊삁援 닔移섏븯瑜 셿솕떆궎뒗 슚怨쇰 솗씤븯떎(Fig. 3). 諛깊삁援ъ쓽 援ъ꽦슂냼씤 由쇳봽援 諛 샇以묎뎄쓽 寃쎌슦, CP뿉 쓽빐 삁븸 궡 鍮꾩쑉씠 蹂솕릺뒗 寃껋쓣 솗씤븷 닔 엳뿀떎. 젙긽援곌낵 鍮꾧탳 떆 由쇳봽援 諛 샇以묎뎄뒗 鍮꾩쑉씠 쑀쓽誘명븯寃 媛먯냼븯쑝굹(P<0.05), 李몃떦洹 異붿텧臾 닾뿬援곗쓽 寃쎌슦 CP 닾뿬濡 쑀룄맂 媛먯냼 쁽긽씠 쉶蹂듬릺뒗 寃껋쓣 솗씤븯떎. 듅엳 怨좊냽룄 李몃떦洹 異붿텧臾(100 mg/kg) 닾뿬援곗뿉꽌 CP뿉 쓽빐 븯맂 由쇳봽援 諛 샇以묎뎄뒗 鍮꾩쑉씠 쑀쓽꽦 엳寃 利앷릺뒗 寃껋쓣 솗씤븯떎(Fig. 3).

Fig. 3. Effects of AGN on blood components in cyclophosphamide-induced immunosuppressed mice. At the end of the experiment, blood cell count test was conducted by blood analyzer. Changes in white blood cell, lymphocytes and neutrophil. Data were represented in the mean ± S.D. and results were analyzed by Tukey post hoc test (#P < 0.05 versus control, *P < 0.05 versus CP alone).

硫댁뿭븯 紐⑤뜽뿉꽌 鍮꾩옣湲곕뒫 蹂솕뿉 븳 李몃떦洹 異붿텧臾쇱쓽 쁺뼢

CP濡 쑀룄맂 硫댁뿭븯 紐⑤뜽뿉꽌 李몃떦洹 異붿텧臾쇱씠 鍮꾩옣쓽 湲곕뒫 蹂솕뿉 誘몄튂뒗 쁺뼢쓣 痢≪젙븯湲 쐞븯뿬 媛 떎뿕援곗쓽 鍮꾩옣 議곗쭅 蹂묐쓣 愿李고븯떎. 젙긽援곗쓽 鍮꾩옣 議곗쭅 由쇳봽냼젅(lymph nodule, LN)씠 媛옣옄由 援ъ뿭(marginal zone, MZ)뿉 몮윭떥뿬 엳怨 쟻깋닔吏(red pule, RP)怨 寃쎄퀎媛 紐낇솗븯寃 援щ텇맂 寃껋쑝濡 愿李곕릺뿀떎. CP 닾뿬援곗쓽 寃쎌슦, 由쇳봽냼젅 媛옣옄由 援ъ뿭쓽 寃쎄퀎媛 紐낇솗븯吏 븡怨 쟻깋닔吏덉뿉꽌 쓳異뺣맂 꽭룷뱾씠 愿李곕릺怨 洹 諛곗뿴 삉븳 留ㅼ슦 遺덇퇋移숉븳 寃껋쑝濡 굹궗떎. 諛섎㈃ 李몃떦洹 異붿텧臾 닾뿬援곗뿉꽌뒗 CP濡 넀긽맂 由쇳봽냼젅쓽 媛옣옄由 援ъ뿭씠 쉶蹂듬릺怨 쟻깋닔吏덉쓽 꽭룷 쓳異 쁽긽씠 샇쟾릺뒗 寃껋쓣 솗씤븯떎(Fig. 4).

Fig. 4. Effects of AGN on histological feature of spleen in cyclophosphamide-induced immunosuppressed mice. Spleens were stained with H&E reagent and ob-served by optical microscope. (A) Control; (B) CP; (C) CP+AGN 10 mg/kg; (D) CP+AGN 100 mg/kg. Original magnifi-cation: ×100. scale bar = 100 μm.

鍮꾩옣 議곗쭅 궡 궗씠넗移댁씤 蹂솕뿉 븳 李몃떦洹 異붿텧臾쇱쓽 쁺뼢

CP 닾뿬濡 쑀룄맂 硫댁뿭븯 紐⑤뜽뿉꽌 李몃떦洹 異붿텧臾쇱씠 鍮꾩옣 궡 궗씠넗移댁씤 蹂솕뿉 誘몄튂뒗 쁺뼢 痢≪젙븯떎. IL-2, IFN-γ 諛 TNF-α 諛쒗쁽뿉 븳 李몃떦洹 異붿텧臾쇱쓽 슚怨쇰 痢≪젙븯湲 쐞븯뿬 媛 떎뿕援곗쓽 鍮꾩옣 議곗쭅뿉꽌 떒諛깆쭏쓣 異붿텧 諛 젙웾 썑 ELISA 떎뿕踰뺤쑝濡 痢≪젙븯떎. IL-2쓽 寃쎌슦, CP 닾뿬援곗 0.46±0.08 pg/mL濡 젙긽援(1.02±0.09 pg/mL)怨 鍮꾧탳뻽쓣 븣 쑀쓽誘명븯寃 媛먯냼븯뒗 寃껋쓣 솗씤븯떎(P<0.05). 李몃떦洹 異붿텧臾 닾뿬援(100 mg/kg)쓽 IL-2 닔移섎뒗 0.62±0.09 pg/mL濡 CP濡 媛먯냼맂 IL-2 諛쒗쁽뼇씠 쑀쓽쟻쑝濡 利앷릺뒗 寃껋쓣 솗씤븯떎. IFN-γ쓽 寃쎌슦, CP 닾뿬援곗뿉꽌 1.80±0.18 pg/mL濡 젙긽援(3.22±0.20 pg/mL)蹂대떎 쑀쓽꽦 엳寃 媛먯냼븯怨(P<0.05), 李몃떦洹 異붿텧臾(100 mg/kg) 닾뿬援곗뿉꽌뒗 2.71±0.21 pg/mL濡 CP 닾뿬(1.80±0.18 pg/mL)濡 媛먯냼맂 IFN-γ 닔以씠 쑀쓽꽦 엳寃 利앷릺뒗 寃껋쓣 솗씤븯떎. TNF-α쓽 寃쎌슦, CP 닾뿬援곗 2.51±0.28 pg/mL濡 젙긽援(2.87±0.19 pg/mL) 蹂대떎 닔移섎뒗 媛먯냼뻽吏留 쑀쓽꽦 뾾뿀떎. 李몃떦洹 異붿텧臾 닾뿬援(100 mg/kg)쓽 TNF-α 닔移섎뒗 2.65±0.35 pg/mL濡 CP 닾뿬援(2.51±0.28 pg/mL)怨 鍮꾧탳 떆 쑀쓽븳 李⑥씠瑜 굹궡吏 븡븯떎(Fig. 5).

Fig. 5. Effects of AGN on cytokines in cyclophosphamide-induced immunosuppressed mice. At the end of the experiment, the spleen tissues were excised and homogenized. Following protein quantification, the levels of IL-2, IFN-γ and TNF-α in spleen tissue were assayed using ELISA assay kits. Data were represented in the mean ± S.D. and results were analyzed by Tukey post hoc test (#P < 0.05 versus control, *P < 0.05 versus CP alone).
怨 李

硫댁뿭怨꾨뒗 쇅遺뿉꽌 쑀엯릺뒗 떎뼇븳 媛먯뿼썝쑝濡쒕꽣 泥대궡瑜 蹂댄샇븯뒗 諛⑹뼱떆뒪뀥쑝濡 硫댁뿭湲곕뒫씠 븯맂 긽깭뿉꽌뒗 硫댁뿭諛섏쓳쓽 遺덇퇏삎쑝濡 븫, 쟾뿼蹂, 븣젅瑜닿린 벑쓽 떎뼇븳 吏덈퀝씠 쑀諛쒕맂떎. 뵲씪꽌 嫄닿컯븳 씤泥대 쑀吏븯湲 쐞빐 硫댁뿭젰 利앹쭊쓣 쐞븳 떎뼇븳 湲곕뒫꽦 냼옱 諛쒓뎬 뿰援ъ쓽 븘슂꽦씠 吏냽쟻쑝濡 슂援щ릺怨 엳떎. 蹂 뿰援щ뒗 CP濡 쑀룄맂 硫댁뿭븯 紐⑤뜽뿉꽌 李몃떦洹 異붿텧臾쇱쓽 硫댁뿭議곗젅 湲곕뒫뿉 븳 슚뒫쓣 룊媛븯떎. 떎뿕 寃곌낵, 李몃떦洹 異붿텧臾쇱쓽 닾뿬뒗 CP濡 넀긽맂 硫댁뿭湲곕뒫쓣 솢꽦솕븯뿬 媛먯냼맂 泥댁쨷 諛 鍮꾩옣, 쓨꽑쓽 議곗쭅以묐웾, 삁援ъ꽭룷 援ъ꽦, 삁븸 궡 硫댁뿭愿젴 궗씠넗移댁씤 븿웾 벑 쟾諛섏쟻쑝濡 硫댁뿭湲곕뒫씠 쉶蹂듬릺뒗 寃껋쑝濡 솗씤븯떎. 씠윴 寃곌낵뒗 李몃떦洹 異붿텧臾쇱씠 硫댁뿭利앹쭊쓣 쐞븳 湲곕뒫꽦 냼옱濡쒖꽌쓽 솢슜媛뒫꽦쓣 솗씤븷 닔 엳뿀떎.

CP뒗 諛깊삁蹂, 궃냼븫 諛 쑀諛⑹븫 벑 떎뼇븳 醫낆뼇 移섎즺젣濡 궗슜릺怨 엳쑝硫 洹 遺옉슜쑝濡 泥댁쨷 媛먯냼 諛 硫댁뿭湲곌쓽 湲곕뒫 옣븷 벑씠 蹂닿퀬릺怨 엳떎(Gladstone et al., 2002). 蹂 뿰援ъ뿉꽌룄 CP 닾뿬援곗 젙긽援곗뿉 鍮꾪빐 쑀쓽꽦 엳뒗 泥댁쨷 媛먯냼瑜 솗씤븯怨, 諛섎㈃ 李몃떦洹 異붿텧臾 닾뿬援곗뿉꽌뒗 CP濡 媛먯냼맂 泥댁쨷 蹂솕媛 쉶蹂듬릺뒗 寃껋쓣 솗씤븯떎(Fig. 1). 삉븳 CP濡 泥대궡 硫댁뿭븯瑜 쑀룄븯寃 릺硫, 硫댁뿭湲곌씤 鍮꾩옣 諛 쓨꽑쓽 以묐웾씠 媛먯냼맂떎뒗 寃껋씠 蹂닿퀬맂 諛붽 엳떎. CP 닾뿬 硫댁뿭븯 援곌낵 李몃떦洹 異붿텧臾 닾뿬援곗뿉꽌 鍮꾩옣, 쓨꽑쓽 以묐웾쓣 痢≪젙븳 寃곌낵, CP濡 媛먯냼맂 鍮꾩옣怨 쓨꽦쓽 以묐웾씠 李몃떦洹 닾뿬援곗뿉꽌 쑀쓽쟻쑝濡 利앷븯떎. CP뿉 쓽빐 쑀룄릺뒗 硫댁뿭븯뒗 삁븸 議곗꽦쓽 蹂솕瑜 씪쑝궎硫 쟻삁援, 諛깊삁援 臾쇰줎 由쇳봽援ъ 샇以묎뎄 媛먯냼瑜 쑀룄븯뒗 寃껋쑝濡 븣젮졇 엳떎. 蹂 뿰援 寃곌낵, CP 닾뿬 硫댁뿭븯 援곗뿉꽌 삁以 諛깊삁援 닔, 由쇳봽援 諛 샇以묎뎄쓽 鍮꾩쑉씠 젙긽援곗뿉 鍮꾪빐 쁽븳 媛먯냼媛 솗씤릺뿀쑝硫, 李몃떦洹 異붿텧臾 닾뿬援곗뿉꽌뒗 CP뿉 쓽빐 쑀룄릺뒗 삁븸븰쟻 빐옉슜씠 셿솕릺뒗 寃껋쓣 솗씤븯떎(Fig. 3).

李몃떦洹 異붿텧臾 닾뿬媛 CP濡 쑀룄맂 硫댁뿭湲곌쓽 湲곕뒫 븯뿉 븳 쁺뼢쓣 痢≪젙븯湲 쐞븯뿬 鍮꾩옣 議곗쭅쓽 삎깭쟻씤 蹂솕瑜 愿李고븯떎. 議곗쭅蹂묐━븰쟻 遺꾩꽍 寃곌낵, 젙긽援곗쓽 鍮꾩옣 議곗쭅 由쇳봽냼젅씠 媛옣옄由 援ъ뿭뿉 몮윭떥뿬 엳怨 쟻깋닔吏덇낵 寃쎄퀎媛 紐낇솗븯寃 援щ텇맂 寃껋쑝濡 愿李곕릺뿀떎. CP援곗쓽 寃쎌슦, 由쇳봽냼젅 媛옣옄由 援ъ뿭쓽 寃쎄퀎媛 紐낇솗븯吏 븡怨 쟻깋닔吏덉뿉꽌 쓳異뺣맂 꽭룷뱾씠 愿李곕릺怨 洹 諛곗뿴 삉븳 留ㅼ슦 遺덇퇋移숉븳 寃껋쑝濡 굹궗떎. 諛섎㈃ 李몃떦洹 異붿텧臾 닾뿬援곗뿉꽌뒗 議곗쭅 궡 媛옣옄由 援ъ뿭씠 꽑紐낇븯寃 愿李곕릺怨 쟻깋닔吏덉쓽 꽭룷 쓳異 쁽긽씠 샇쟾릺뒗 寃껋쓣 솗씤븯떎(Fig. 4).

硫댁뿭꽭룷뿉꽌 遺꾨퉬릺뒗 궗씠넗移댁씤 떎뼇븳 硫댁뿭湲곕뒫쓣 議곗젅븯뒗 寃껋쑝濡 蹂닿퀬릺뼱 엳떎(Jiang et al., 2010). 듅엳 TNF-α뒗 硫댁뿭꽭룷씤 T 由쇳봽援ъ쓽 遺꾪솕瑜 쑀룄븯怨, IL-2뒗 T cell 遺꾪솕 諛 꽭룷꽦 硫댁뿭쓣 솢꽦솕 떆궎硫 Th1 꽭룷뿉꽌 깮꽦릺뒗 IFN-γ뒗 떇꽭룷쓽 湲곕뒫쓣 媛뺥솕븯뒗 寃껋쑝濡 븣젮졇 엳떎. CP쓽 硫댁뿭븯 쑀룄뒗 硫댁뿭꽭룷뿉꽌 궗씠넗移댁씤쓽 깮꽦쓣 뼲젣떆궎뒗 寃껋쑝濡 蹂닿퀬맂 諛 엳떎(Logani et al., 2012). 蹂 뿰援ъ뿉꽌뒗 CP 닾뿬濡 쑀룄맂 硫댁뿭븯 紐⑤뜽뿉꽌 李몃떦洹 異붿텧臾쇱씠 鍮꾩옣 궡 궗씠넗移댁씤 諛쒗쁽 蹂솕뿉 誘몄튂뒗 쁺뼢쓣 ELISA 遺꾩꽍踰뺤쓣 씠슜븯뿬 痢≪젙븯떎. 떎뿕 寃곌낵, CP 닾뿬援곗뿉꽌뒗 鍮꾩옣 議곗쭅 궡 IL-2 IFN-γ 諛쒗쁽웾씠 쑀쓽꽦 엳寃 媛먯냼릺뿀怨, 李몃떦洹 異붿텧臾 닾뿬援곗뿉꽌뒗 븯맂 IL-2 IFN-γ 諛쒗쁽웾씠 쉶蹂듬릺뒗 寃껋쓣 솗씤븷 닔 엳뿀떎. 븯吏留 鍮꾩옣 議곗쭅 궡 TNF-α 諛쒗쁽 蹂솕뿉 븳 李몃떦洹 異붿텧臾쇱쓽 슚怨쇰뒗 CP 닾뿬援곌낵 鍮꾧탳 떆 쑀쓽븳 李⑥씠瑜 蹂댁씠吏 븡븯떎. 씠윭븳 寃곌낵뒗 李몃떦洹 異붿텧臾쇱 硫댁뿭湲곕뒫 諛 議고삁옉슜 媛쒖꽑 슚怨쇨 엳쓬쓣 利앸챸븯뒗 怨쇳븰쟻 洹쇨굅媛 맆 닔 엳쓣 寃껋쑝濡 궗猷뚮맂떎.

寃곕줎쟻쑝濡 蹂 뿰援щ뒗 CP濡 쑀룄맂 硫댁뿭븯 紐⑤뜽뿉꽌 李몃떦洹 異붿텧臾쇱쓽 硫댁뿭議곗젅 湲곕뒫뿉 븳 슚뒫쓣 룊媛븳 寃곌낵濡 李몃떦洹 異붿텧臾쇱쓽 硫댁뿭湲곕뒫 媛쒖꽑 슚뒫쓣 怨쇳븰쟻쑝濡 엯利앺븯떎. 듅엳 李몃떦洹 異붿텧臾쇱쓽 寃쎌슦 CP 닾뿬濡 쑀룄맂 泥댁쨷 媛먯냼, 硫댁뿭湲곌(鍮꾩옣 諛 쓨꽑)쓽 以묐웾 蹂솕 諛 삁援ъ꽭룷쓽 媛먯냼媛 媛쒖꽑맖쓣 솗씤븯떎. 삉븳 CP濡 쑀룄맂 鍮꾩옣 議곗쭅쓽 넀긽 諛 궗씠넗移댁씤 諛쒗쁽웾 媛먯냼媛 李몃떦洹 異붿텧臾쇱뿉 쓽빐 쉶蹂듬릺뒗 寃껋쑝濡 솗씤븯떎. 뵲씪꽌 李몃떦洹 異붿텧臾쇱 CP 닾뿬濡 쑀룄맂 硫댁뿭븯 紐⑤뜽뿉꽌 硫댁뿭利앹쭊 솢꽦씠 엯利앸릺뿀怨 異뷀썑 硫댁뿭議곗젅 젣뭹 媛쒕컻뿉 湲곕뒫꽦 냼옱濡 솢슜 媛뒫븷 寃껋쑝濡 궗猷뚮맂떎.

ACKNOWLEDGEMENT

None.

CONFLICT OF INTEREST

The authors declare that they have no conflict of interest.

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