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Immunoactivity-Enhancing Effect of Fermented Samultang Porridge in an Animal Model of Cyclophospahmide-Induced Immunodeficiency
Biomed Sci Letters 2023;29:168-177
Published online September 30, 2023;  https://doi.org/10.15616/BSL.2023.29.3.168
© 2023 The Korean Society For Biomedical Laboratory Sciences.

Ji-Hye Oh1,*, Seung-Hwa Baek1,**, Hak-Joo Choi2,*** and Seock-Yeon Hwang1,†,****

1Department of Biomedical Laboratory Science, Daejeon University, Daejeon 34520, Korea
2Department of Herbology, Collge of Korean Medicine, Daejeon University, Daejeon 34520, Korea
Correspondence to: Seock-Yeon Hwang. Department of Biomedical Laboratory Science, Daejeon University, Daejeon 34520, Korea.
Tel: +82-10-9429-4175, Fax: +82-42-283-8808, e-mail: syhwang@dju.kr
*Ungraduate student, **Graduate student, ***Researcher, ****Professor.
Received September 11, 2023; Revised September 22, 2023; Accepted September 22, 2023.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
 Abstract
Recently, as a health problem of the elderly in an aging society, the risk of nutritional imbalance and weakening of immunity due to deterioration of masticatory function has been mentioned. In order to solve this problem, this study was conducted to investigate the effect of cyclophosphamide (CPA)-induced immunosuppression in mice induced by fermented samultang (FST) porridge on the markers related to immune activity function. ICR Mouse was divided into 6 groups of 7 animals each. Experimental groups were set as normal control group, CPA-administration group, positive control group, and FST-administration experimental group (0.25%, 0.5%, 1.0%). In groups except for the normal control group, 100 μL of CPA dissolved in 0.9% NaCl at a concentration of 150 mg/kg was injected twice at the start of the experiment and after 3 days to induce immunosuppression. As a result of analyzing the cell proliferation capacity of splenocytes, all B and T cells decreased in the CPA-administered group and increased in a concentration-dependent manner in the FST-administered group. In addition, IgA measured to evaluate the effect of improving immunity showed high values in medium and high concentration FST (P<0.05). These results can be expected as an effective solution to improve the nutritional imbalance of the elderly.
Keywords : Aging society, Cyclophosphamide, Fermented Samultang (FST), Immune-Enhancing
꽌 濡

쁽옱 슦由щ굹씪뒗 寃쎌젣諛쒖쟾 諛 蹂닿굔쓽猷 吏 뼢긽 벑쑝濡 룊洹좎닔紐낆씠 뒛뼱궗吏留 異쒖궛 諛 寃고샎 湲고뵾, 끂씤 씤援ъ쓽 利앷濡 怨좊졊솕媛 쁽 궗쉶뿉꽌 以묒슂븳 臾몄젣濡 씤떇릺怨 엳쑝硫, 끂뀈湲 룞븞 吏덈퀝 諛 옣븷媛 뾾뒗 嫄닿컯븳 궣뿉 愿떖쓣 湲곗슱씠怨 엳떎(Mumma and Quinton, 1970; Bang and Yang, 2008). 理쒓렐 湲됯꺽븳 怨좊졊솕濡 씤빐 끂씤쓽 떊泥 諛 씤吏湲곕뒫 븯 벑 끂씤 嫄닿컯怨 愿젴맂 臾몄젣媛 利앷븯怨 엳떎(Kim and Joo, 2015). 듅엳 援ш컯 긽깭 븙솕濡 옉湲곕뒫씠 뼥뼱졇 냼솕怨쇱젙뿉꽌 쁺뼇냼 꽠痍④ 遺議깊빐吏硫댁꽌 쁺뼇遺덇퇏삎쓣 珥덈옒븳떎怨 蹂닿퀬릺怨 엳떎(Choi and Mun, 2018). 援誘쇨굔媛뺥넻怨꾩옄猷뚯뿉 뵲瑜대㈃, 65꽭 씠긽 끂씤뱾쓽 옉 遺덊렪 샇냼쑉씠 33.1%씠怨, 援ш컯湲곕뒫 젣븳쑉씠 34.8%濡 굹궗쑝硫, 쟾泥 끂씤 씤援ъ쓽 빟 30% 씠긽씠 옉 슫룞뿉 遺덊렪쓣 寃り퀬 엳떎怨 諛쒗몴릺뿀떎(Oh et al., 2022). 꽑뻾뿰援 議곗궗 寃곌낵, 옉 뒫젰씠 븯맂 끂씤 젙긽 끂씤蹂대떎 뿉꼫吏 꽠痍⑥쑉씠 媛먯냼븯怨, 留롮 옉 슫룞씠 븘슂濡 븯뒗 怨쇱씪 諛 梨꾩냼 꽠痍⑥쑉씠 媛먯냼븳 寃껋쑝濡 굹궗떎(Mumma and Quinton, 1970). 씠泥섎읆, 옉옉슜씠 렪븳 쓬떇쓣 二쇰줈 꽑깮븯뒗 떇씠 꽠痍 뒿愿씠 삎꽦릺硫댁꽌 끂씤쓽 떊泥닿린뒫 븯, 젙떊쟻 뒪듃젅뒪, 泥댁쨷 媛먯냼瑜 쑀諛쒗븯寃 맂떎. 삉븳, 끂솕媛 吏꾪뻾맖뿉 뵲씪 泥대궡 빆泥 깮꽦씠 媛먯냼븯怨, 硫댁뿭꽭룷媛 쇅遺 빆썝뿉 빟븯寃 諛섏쓳븯硫, 媛먯뿼뿉 痍⑥빟빐졇 吏덈퀝 끂異쒖뿉 쁺뼢쓣 겮移섍쾶 맂떎. 븯굹쓽 삁濡, 2017뀈 援誘쇨굔媛뺣낫뿕怨듬떒쓽 諛쒗몴뿉 쓽븯硫, 끂씤뿉寃뚯꽌 쓷븯寃 굹굹뒗 留뚯꽦 옄媛硫댁뿭吏덊솚씤 瑜섎쭏떚뒪 愿젅뿼(Song, 2021)쓽 寃쎌슦 留ㅻ뀈 利앷븯뒗 異붿꽭瑜 蹂댁씠怨 엳쑝硫, 뿰룊洹 利앷쑉씠 40 50뒗 媛곴컖 2.5%씤 諛섎㈃, 70뒗 6.0%, 80 씠긽 9.0%濡 利앷쑉씠 넂븘吏怨 엳떎(Park and Lee, 2017). 씠泥섎읆, 끂뀈씠 릺硫댁꽌 痍⑥빟빐吏뒗 泥대궡 硫댁뿭젰 븯瑜 삁諛⑺븯湲 쐞빐, 쟻젅븳 쁺뼇 꽠痍⑤뒗 끂뀈쓽 硫댁뿭젰 利앹쭊뿉 以묒슂븳 寃곗젙슂씤씠 맂떎(Lee, 2008).

쁽옱, 슦由щ굹씪蹂대떎 30뀈 븵꽌 怨좊졊솕媛 吏꾪뻾릺뼱 꽭怨 옣닔援媛濡 븣젮吏 씪蹂몄 옉, 뿰븯(궪궡) 怨ㅻ怨 吏덈퀝 삁諛⑹뿉 븳 끂졊 嫄닿컯떇뭹媛쒕컻씠 솢諛쒗븯寃 씠猷⑥뼱吏怨 엳吏留, 슦由щ굹씪쓽 寃쎌슦 怨좊졊 移쒗솕 떇뭹 궛뾽떆옣씠 珥덇린 떒怨꾨줈, 怨좊졊옄뱾쓽 깮由ъ쟻 듅꽦뿉 留욌뒗 젣뭹媛쒕컻쓽 룄엯씠 뒭뼱吏 듅吏뺤씠 엳떎. 삉븳, 理쒓렐源뚯쓽 硫댁뿭利앹쭊 솢꽦 뿰援 遺遺꾩씠 硫댁뿭뼲젣젣쓽 遺옉슜씠굹 룆꽦쓣 寃쎄컧떆궗 닔 엳뒗 臾쇱쭏媛쒕컻, 씤泥 硫댁뿭議곗젅 솢꽦뿉 誘몄튂뒗 쁺뼢뿉 吏묒쨷릺뼱, 듅젙 뿰졊痢듭뿉 븳 留욎땄삎 뿰援щ뒗 誘명씉븳 떎젙씠떎(Lee et al., 2007; Yang et al., 2015). 씠뿉, 끂씤쓽 옉湲곕뒫뿉 룄쓣 以 닔 엳뒗 옄泥 媛쒕컻 二쎌뿉 硫댁뿭젰 利앹쭊 슚怨쇰 蹂 닔 엳떎怨 븣젮吏 諛쒗슚궗臾쇳깢 二쎌쓣 泥④븯뿬 떎뿕븯怨좎옄 븳떎. 諛쒗슚궗臾쇳깢 二쎌 떦洹, 泥쒓턿, 닕吏솴, 諛깆옉빟쑝濡 援ъ꽦릺뼱 엳쑝硫, 븳諛⑹쿂諛 以 媛옣 湲곕낯쟻씤 泥섎갑쑝濡 媛깅뀈湲 옣븷, 硫댁뿭븯 벑쓽 移섎즺젣濡 삁濡쒕꽣 꼸由 궗슜맂떎怨 븣젮졇 엳떎(Lee et al., 2010; Hwang et al., 2022).

諛쒗슚궗臾쇳깢 二쎌쓽 援ъ꽦꽦遺 以 떦洹(Angelica gigas)뒗 誘몃굹由ш낵뿉 냽븯뒗 떎뀈깮 蹂 珥덉씤 李몃떦洹 肉뚮━濡 1~2뀈깮 肉뚮━瑜 嫄댁“븳 寃껋씠떎(Huh et al., 2022). 떦洹쓽 슚뒫 빆뿼, 吏꾪넻, 硫댁뿭利앷컯뿉 슚뒫씠 엳뒗 寃껋쑝濡 븣젮졇 엳떎(Lee et al., 2009). 삉븳, 꽑뻾뿰援ъ뿉 뵲瑜대㈃, 硫댁뿭꽭룷 솢꽦臾쇱쭏쓽 깮꽦議곗젅쓣 넻빐 硫댁뿭議곗젅뿉 슚뒫씠 엳떎怨 븣젮졇 엳떎(Jeon and Kim, 2022).

泥쒓턿(Cnidium officinal) 誘몃굹由ш낵뿉 냽븯뒗 떎뀈깮 珥덈낯씠怨 以묎뎅, 븳援, 씪蹂몄뿉꽌 二쇰줈 옱諛곕맂떎(Choi et al., 2012). 泥쒓턿 삁愿쓣 솗옣떆耳 삁븸닚솚뿉 룄쓣 二쇨퀬, 湲곗슫쓣 닚議곕∼寃 넻븯寃 븯뿬, 삁濡쒕꽣 떎뼇븳 吏덊솚쓽 삁諛⑷낵 移섎즺젣濡 꼸由 궗슜릺뼱 솕떎(Zhou and Qu, 2009). 理쒓렐 뿰援ъ뿉꽌 泥쒓턿異붿텧臾쇱쓽 깮由ъ옉슜쑝濡 빆븫, 吏꾪넻, 빆뿼(Lee et al., 2019), 빆諛뺥뀒由ъ븘(Sim and Shin, 2014) 벑쑝濡 옉슜븳떎怨 蹂닿퀬릺怨 엳떎.

닕吏솴(Rehmanniae Radix Preparata) 떦洹, 泥쒓턿, 옉빟 벑怨 븿猿 4 븳빟옱濡 빐뿴젣, 吏삁젣, 鍮덊삁, 룓寃고빑 벑뿉 궗슜릺뒗 以묒슂븳 븳빟옱씠떎(Bae et al., 2015). 삉븳, 삁븬議곗젅, 議고삁옉슜, 븫꽭룷 꽦옣 빐 벑쓽 留뚯꽦蹂묒뿉 꼸由 벐씠怨, 硫댁뿭젰 利앷쓽 슚뒫쓣 媛吏怨 엳떎(Kim, 2011).

諛깆옉빟(Paeonia japonica) 옉빟怨쇱쓽 옉빟 냽뿉 냽븯뒗 떎뀈깮떇臾쇰줈 肉뚮━瑜 嫄댁“븯뿬 빟옱瑜 궗슜븳떎. 諛깆옉빟쓽 二쇱슂 옉슜쑝濡 빆끂솕, 빆뿼利, 빆洹좎옉슜, 빆븫, 硫댁뿭湲곕뒫 利앹쭊 벑뿉 슚怨쇨 蹂닿퀬릺怨 엳떎(Lee et al., 2021; Park et al., 2001).

뵲씪꽌 蹂 뿰援щ뒗 끂씤쓽 옉, 뿰븯, 硫댁뿭湲곕뒫 븯 臾몄젣瑜 빐寃고븯湲 쐞븯뿬 (二)꽌李쎌궛뾽쑝濡쒕꽣 젣怨듬컺 二쇱썝猷뚯씤 떗怨좉린 二쎌뿉 쐞쓽 湲곕뒫쓣 媛議뚮떎怨 븣젮吏 諛쒗슚궗臾쇳깢 二쎌쓣 씠슜븯뿬 Cyclophosphamide 쑀룄 硫댁뿭뼲젣 룞臾쇰え뜽뿉꽌 硫댁뿭젰 利앹쭊 슚怨쇨 엳뒗吏 寃利앺븯怨, 硫댁뿭 옣湲 諛 泥대궡 硫댁뿭議곗젅 愿젴 吏몴뿉 誘몄튂뒗 쁺뼢쓣 議곗궗븯怨좎옄 븯떎.

옱猷 諛 諛⑸쾿

諛쒗슚궗臾쇳깢 二쎌쓽 젣議

蹂 뿰援ъ뿉 궗슜맂 諛쒗슚궗臾쇳깢 二쎌 (二)꽌李쎌궛뾽쑝濡쒕꽣 젣怨듬컺븘 吏꾪뻾븯떎. 諛쒗슚궗臾쇳깢쓽 硫댁뿭슚뒫 솢꽦 寃利앹쓣 쐞빐 1.0%, 0.5%, 0.25% 諛고빀鍮꾨줈 硫댁뿭슚뒫 솢꽦 寃利앹쓣 떆뻾븯怨, 諛쒗슚궗臾쇳깢쓽 벖留쏆쓣 셿솕븯뒗 理쒖쟻쓽 諛고빀鍮꾨 꽦옣븯떎. 二쇱썝猷뚯씤 떗怨좉린뒗 깮臾쇱쓣 궗슜븯怨, 諛쒗슚궗臾쇳깢怨 諛고빀 떆 떗怨좉린瑜 癒쇱 씡엺 썑 샎빀븯뿬 떎뿕쓣 吏꾪뻾븯떎. 諛쒗슚궗臾쇳깢 二쎌쓽 議곗꽦 鍮꾩쑉 泥쒓턿, 옉빟, 떦洹, 닕吏솴쑝濡 媛 븳빟옱瑜 1:1:1:1쓽 鍮꾩쑉뿉 珥 븳빟옱 뼇뿉 15諛곗쓽 젙젣닔瑜 닾엯븯뿬 1李 異붿텧怨 2李 異붿텧쓣 넻빐 냽異뺥븯쑝硫, L. delbruekii, L. acidophilus, L. casei, [0085] S. thermophilus 諛 Yo-mix쓽 5媛吏쓽 洹좎쓣 젒醫낆떆耳 30~40꼦뿉꽌 138떆媛 諛쒗슚떆耳곕떎. 理쒖쥌떆猷뚮뒗 룞寃 嫄댁“맂 삎깭濡 쟾떖諛쏆븯쑝硫, 깋옣怨좎뿉 蹂닿븯뿬 硫멸퇏利앸쪟닔뿉 끃뿬 궗슜븯떎.

떎뿕룞臾

泥댁쨷 33.2짹1.8 g쓽 6 二쇰졊 닔而 ICR mouse瑜 (二)씪삩諛붿씠삤(Yongin City, Gyeong-gido)濡쒕꽣 援ъ엯븯뿬 씪二쇱씪媛 쟻쓳 썑 떎뿕뿉 씠슜븯떎. 떎뿕룞臾쇱쓽 궗쑁 삩룄뒗 23짹 1꼦, 뒿룄 50짹5%, 紐낆븫二쇨린 12떆媛꾩쑝濡 빆삩, 빆뒿씠 쑀吏릺뒗 궗쑁떎뿉꽌 궗쑁븯떎. 궗猷뚮뒗 떎뿕룞臾쇱슜 怨좏삎궗猷(Purina, Seongnam City, Korea)瑜 異⑸텇엳 怨듦툒븯怨, 떇닔룄 젣븳 뾾씠 怨듦툒븯떎. 蹂 룞臾 떎뿕뿉 愿젴맂 紐⑤뱺 떆뿕 怨쇱젙怨 젅李⑤뒗 쟾븰援 룞臾 떎뿕 쐞썝쉶쓽 궗쟾떖쓽 쑄由 洹쒖젙쓣 以닔븯뿬 닔뻾븯떎(DJUAIRB2018-56).

떎뿕 諛⑸쾿

룞臾쇨뎔 꽕젙 諛 닾뿬: 씪二쇱씪쓽 닚솕 湲곌컙쓣 嫄곗튇 썑 룊洹 泥댁쨷怨 洹쇱젒븳 媛쒖껜뱾쓣 꽑諛쒗븯쑝硫, 臾댁옉쐞踰뺤쓣 씠슜븯뿬 援곕떦 7留덈━뵫 6援곗쑝濡 굹늻뿀떎. 떎뿕援곗 젙긽議곌뎔(Normal Control, 씠븯 N.C), Cyclophosphamide(씠븯 CPA) 떒룆 닾뿬援(immunsuppression, 씠븯 I), CPA 룞寃 嫄댁“맂 湲곕낯二(10% 냽룄, D.W) 닾뿬援곗뿉 諛쒗슚궗臾쇳깢 蹂묓뻾 닾뿬 냽룄援(씠븯 BL, 0.25% mg/kg b.w), 以묐냽룄援(씠븯 BM, 0.5% mg/kg b.w), 怨좊냽룄援(씠븯 BH, 1.0% mg/kg 6媛쒖쓽 援곗쑝濡 꽕젙븯떎. 0.9% NaCl뿉 Cyclophosphamide (Sigma aldrich, Korea)瑜 150 mg/kg 냽룄濡 100 關L뵫 떎뿕 떆옉씪怨 3씪 썑뿉 蹂듦컯 닾뿬濡 몢 踰 二쇱궗븯뿬 硫댁뿭뼲젣瑜 쑀룄븯떎. 떆뿕援곗쓽 寃쎌슦 10% 냽룄쓽 湲곕낯 쁺뼇二쎌뿉 냽룄, 以묐냽룄, 怨좊냽룄(0.25%, 0.5%, 1.0%)쓽 냽룄濡 媛곴컖 룞寃 嫄댁“맂 븳빟옱瑜 泥④븯뿬 硫댁뿭뼲젣젣 룞떆 닾뿬瑜 吏꾪뻾븯怨, 10 mL/kg 슜웾쑝濡 珥 14씪媛 寃쎄뎄 닾뿬븯떎.

옣湲 以묐웾 痢≪젙: 떎뿕 떆옉씪怨 醫낅즺씪뿉 떎뿕룞臾 泥댁쨷쓣 痢≪젙븯쑝硫, 떆뿕臾쇱쭏 留덉留 닾뿬媛 걹궃 썑 24떆媛 뮘뿉 isoflurane (JSK, Korea)쑝濡 씉엯留덉랬 썑 蹂듬 룞留μ뿉꽌 梨꾪삁븯怨 硫댁뿭꽭룷쓽 遺꾪솕媛 씪뼱굹뒗 湲곌씤 쓨꽑 諛 鍮꾩옣쓣 닔吏묓븯떎. 媛 媛쒖껜쓽 泥댁쨷뿉 뵲瑜 李⑥씠瑜 以꾩씠湲 쐞빐 理쒖쥌 씗깮 씪쓽 泥댁쨷뿉 븳 긽 以묐웾쓣 궛異쒗븯뿬 諛깅텇쑉(%)濡 硫댁뿭 옣湲 臾닿쾶 蹂솕瑜 굹궡뿀떎.


Relative혻Organ혻Weights혻(%혻of혻body혻weight)=Absolute혻organ혻weightBody혻weight혻at혻sacrifice100

Relative organ weights of ICR mice (% Absolute Organ weight/body weight)


鍮꾩옣꽭룷 遺꾨━: 떎뿕룞臾쇱쓽 씗깮 썑 鍮꾩옣쓣 쟻異쒗븯뿬 二쇰 議곗쭅쓣 젣嫄고빐 以 뮘 젅諛섏쑝濡 젅떒븯뿬 궗슜븯쑝硫, 궓 젅諛섏쓽 議곗쭅 議곗쭅蹂묐━븰쟻 寃궗뿉 궗슜릺뿀떎. 鍮꾩옣쓣 빟 10 mL쓽 RPMI (2%, Gibco, USA)뿉 꽭泥숉븳 뮘, Petri dish뿉 삷寃 遺꾩뇙븳 썑 썝떖遺꾨━湲(4꼦, 1,700 rpm, 5遺, LABOGENE 1248, Korea)瑜 씠슜븯뿬 긽痢듭븸쓣 젣嫄고븯떎. 鍮꾩옣꽭룷븸뿉 븿쑀릺뼱 엳뒗 쟻삁援щ 젣嫄고븯湲 쐞빐 RBC lysis buffer (Sigma aldrich. Korea)瑜 3 mL 泥④븳 뮘 빆삩닔議(37꼦)뿉꽌 5遺꾧컙 諛⑹튂븳 썑 썝떖遺꾨━(4꼦, 1,700 rpm, 5遺) 븯떎. 긽痢듭븸쓣 젣嫄고븳 썑 移⑥쟾맂 鍮꾩옣꽭룷뿉 FBS (10%, Gibco, USA)媛 泥④맂 RPMI-1640 (Gibco, USA) 諛곗븸쓣 泥④븯떎.

鍮꾩옣꽭룷 利앹떇뒫 痢≪젙: 遺꾨━맂 鍮꾩옣꽭룷瑜 5횞106 Cell/ mL媛 릺룄濡 議곗젅븯떎. 遺꾨━맂 鍮꾩옣꽭룷븸쓣 96 well plate뿉 well 떦 90 關L뵫 媛븯怨, 媛 援곕떦 mitogen쑝濡 肄섏뭅굹諛쒕┛ A (Concanavalin A, 씠븯 ConA, 5 關g/mL, Sigma aldrich. Korea), 吏吏덈떎떦瑜(Lipopolysaccharide, 씠븯 LPS, 15 關g/mL, Sigma aldrich. Korea)瑜 10 關L뵫 遺꾩<븯怨 CO2 incubator (37꼦, 5%)뿉꽌 44떆媛 룞븞 諛곗뼇븯떎. 諛곗뼇 썑 MTT (Gibco, USA)瑜 10 關L 媛븯怨 4떆媛 룞븞 諛곗뼇 썑 formazan crystal 삎꽦쓣 쑀룄븯떎. 씠썑 4꼦, 1,500 rpm, 5遺꾧컙 썝떖遺꾨━븯뿬 긽痢듭븸쓣 젣嫄고븯怨 媛 well뿉 150 關L쓽 Dimethyl sulfoxide (DMSO)瑜 媛븯뿬 10遺꾧컙 諛⑹튂븯쑝硫, ELISA reder (Multiskan GO, Thermo SCIENTIFIC, USA)瑜 씠슜븯뿬 540 nm쓽 씉愿묐룄뿉꽌 痢≪젙븯떎.

由쇳봽援 븘삎 寃궗: 由쇳봽援 븘삎 寃궗 痢≪젙쓣 쐞빐 遺꾨━맂 鍮꾩옣꽭룷븸쓣 1횞106 Cell/100 關L媛 릺룄濡 議곗젅븯떎. 議곗젅맂 鍮꾩옣꽭룷븸뿉 FACS Buffer (PBS contain 1% BSA) 1 mL瑜 泥④븳 썑 썝떖遺꾨━(4꼦, 2,000 rpm, 10遺)븯뿬 2쉶 꽭泥숉븯떎. 긽痢듭븸 젣嫄 썑, 1% paraformaldehyde媛 룷븿맂 FACS buffer 200 關L瑜 씠슜빐 pellet쓣 쁽긽떆궎怨 ice뿉꽌 15遺꾧컙 諛섏쓳떆耳곕떎. FACS buffer (PBS contain 1% BSA) 1 mL쓣 씠슜븯뿬 2쉶 꽭泥 썑, 긽痢듭븸쓣 젣嫄고븯怨, pellet쓣 FACS buffer 500 關L뿉 쁽긽떆耳 FACS tube濡 씠룞 썑 痢≪젙븯떎.

硫댁뿭湲濡쒕텋由 痢≪젙: 硫댁뿭湲濡쒕텋由 G (Immunoglobulin G, 씠븯 IgG), 硫댁뿭湲濡쒕텋由 A (Immunoglobulin A, 씠븯 IgA), 硫댁뿭湲濡쒕텋由 M (Immunoglobulin M, 씠븯 IgM) 痢≪젙 Mouse Immunoglobulin ELISA Kit (Cusabio Biotech., Co. Ltd Fannin St, USA)瑜 궗슜븯뿬 떎뿕븯떎. 硫댁뿭湲濡쒕텋由 痢≪젙쓣 쐞빐 媛 Well 떦 Standard 寃泥대 50 關L뵫 遺꾩<븳 뮘, HRP-conjugate瑜 50 關L 異붽븯怨 옒 샎빀 썑 37꼦뿉꽌 60遺 룞븞 諛곗뼇븯떎. 媛 Well쓣 wash buffer瑜 씠슜빐 5踰 꽭泥숉븳 썑 90 關L쓽 TMB substrate瑜 꽔怨 鍮쏆씠 李⑤떒맂 긽깭뿉꽌 37꼦, 20遺꾧컙 諛곗뼇븯떎. 留덉留됱쑝濡 媛 Well뿉 50 關L쓽 stop solution쓣 꽔怨 뵆젅씠듃瑜 샎빀떆궓 썑 ELISA reader (Multiscan GO, Thermo SCIEN TIFIC, USA)뿉꽌 450 nm쓽 씉愿묐룄濡 痢≪젙븯떎.

議곗쭅蹂묐━븰쟻 寃궗: 룞臾 떆뿕 醫낅즺씪뿉 젅痍⑦븳 鍮꾩옣 諛 쓨꽑쓣 10% 以묒꽦 셿異 룷瑜대쭚由곗뿉 24떆媛 怨좎젙븯떎. 怨좎젙맂 議곗쭅 蹂묐━議곗쭅븰쟻 寃궗瑜 쐞븳 넻긽쟻씤 諛⑸쾿쓣 궗슜븯뿬 뙆씪 룷留 썑, 4 關m 몢猿섎줈 뿰냽젅렪 븯떎. 鍮꾩옣 諛 쓨꽑쓽 씪諛섏쟻씤 삎깭 蹂솕瑜 愿李고븯湲 쐞빐 뿰냽젅렪쓣 hematoxylin & eosin (H&E) 뿼깋 썑 愿묓븰쁽誘멸꼍쑝濡 愿李고븯떎.

넻怨꾩쿂由: 議곗쭅솕븰 諛 硫댁뿭議곗쭅솕븰 寃곌낵瑜 젙웾솕븯湲 쐞빐 image J program (Wayne Rasband, National Institutes of Health, Bethesada, MD, USA)쓣 씠슜븯뿬 쁺긽遺꾩꽍쓣 떆뻾븯떎. 紐⑤뱺 떎뿕 寃곌낵뒗 룊洹좉낵 몴以삤李⑤줈 굹궡怨, 媛 援 媛꾩쓽 李⑥씠뒗 SPSS v.20 (SPSS Inc. Chicago, IL. USA)쓣 궗슜븯뿬 Student's t-test濡 遺꾩꽍븯쑝硫, P<0.05 씪 븣 넻怨꾩쟻쑝濡 쑀쓽꽦씠 엳떎怨 뙋떒븯떎.

寃 怨

硫댁뿭 옣湲 以묐웾 痢≪젙

諛쒗슚궗臾쇳깢쓣 븿쑀븳 湲곕낯二 닾뿬뿉 쓽븳 硫댁뿭젰 媛쒖꽑 슚怨쇰 룊媛븯湲 쐞빐 룞臾쇱쓽 鍮꾩옣 諛 쓨꽑 議곗쭅뿉꽌 옣湲 以묐웾쓣 痢≪젙븯떎. 鍮꾩옣쓽 寃쎌슦 젙긽議곌뎔 0.27짹0.02% 鍮꾧탳븯쓣 븣, 硫댁뿭뼲젣援 0.55짹0.04%뿉꽌 쑀쓽븯寃 利앷(P<0.05)븯떎(Fig. 1). 諛섎㈃, 떆뿕臾쇱쭏 닾뿬援곗뿉꽌뒗 냽룄뿉 뵲씪 媛먯냼븯쑝硫 듅엳 BM援곗뿉꽌 0.49짹0.14%濡 媛먯냼븯뒗 寃껋쓣 愿李고븯쑝굹, 쑀쓽꽦 굹굹吏 븡븯떎. 쓨꽑쓽 寃쎌슦 젙긽議곌뎔 0.16짹0.03% 鍮꾧탳븯쓣 븣, 硫댁뿭뼲젣援 0.15짹0.01%濡 媛먯냼븯쑝굹 쑀쓽꽦 굹굹吏 븡븯떎. CPA 諛쒗슚궗臾쇳깢 二 닾뿬援곗쓣 鍮꾧탳븯쓣 븣 쓨꽑쓽 臾닿쾶媛 쟾泥댁쟻쑝濡 利앷븯吏留 쑀쓽꽦 愿李곕릺吏 븡븯떎(Fig. 2).

Fig. 1. Effects of fermented samultang extract on spleen weight rate changes in mouse with immunsuppression induced by cyclophosphamide. N.C: normal control, I: Cyclophosphamide induced immunsuppression, P.C: Cyclophosphamide induced immunsuppression with basic porridge 10%, BL: Cyclophosphamide induced immunsuppression with fermented samultang extract 0.25% in basic porridge 10%, BM: Cyclophosphamide induced immunsuppression with fermented samultang extract 0.5% in basic porridge 10%, BH: Cyclophosphamide induced immunsuppression with fermented samultang extract 1% in basic porridge 10% in male ICR mouse. Data are shown as mean 짹 SD (n=7). Data are shown as mean 짹 SE (n=7). Significant difference from normal group by Student's t-test: *P<0.05.

Fig. 2. Effects of fermented samultang extract on thymus weight rate changes in mouse with immunsuppression induced by cyclophosphamide. Notes on group designation at the same as Fig. 1. Data are shown as mean 짹 SE (n=7).

鍮꾩옣꽭룷 利앹떇뒫 痢≪젙

諛쒗슚궗臾쇳깢쓣 븿쑀븳 湲곕낯二 닾뿬뿉 쓽븳 硫댁뿭젰 媛쒖꽑 슚怨쇰 룊媛븯湲 쐞빐 룞臾쇱쓽 鍮꾩옣꽭룷뿉꽌 B 꽭룷 諛 T 꽭룷 利앹떇뒫쓣 痢≪젙븯떎. B 꽭룷 遺꾩뿴珥됱쭊씤옄씤 LPS瑜 씠슜븳 B 꽭룷 利앹떇뒫쓣 痢≪젙븳 寃곌낵, 젙긽議곌뎔 6.08짹7.80 pg/mL怨 鍮꾧탳븯쓣 븣, 硫댁뿭뼲젣援곗뿉꽌 0.91짹0.68 pg/mL濡 쁽븯寃 媛먯냼븯쑝굹, 쑀쓽꽦 굹굹吏 븡븯떎. 硫댁뿭뼲젣援곌낵 鍮꾧탳븯쓣 븣, 諛쒗슚궗臾쇳깢 二쎌쓣 닾뿬븳 紐⑤뱺 援곗뿉꽌 냽룄 쓽議댁쟻쑝濡 利앷븯뒗 슚怨쇰 蹂댁쑝硫, 듅엳 BH援곗뿉꽌 5.24짹3.47 pg/mL濡 쑀쓽븯寃 利앷(P<0.05)븯떎(Fig. 3). 삉븳, 湲곕낯二쎌쓣 닾뿬뻽쓣 븣蹂대떎, 諛쒗슚궗臾쇳깢 二쎌쓣 以묐냽룄, 怨좊냽룄濡 닾뿬븳 援곗뿉꽌 젙긽議곌뎔怨 쑀궗븷 젙룄濡 利앷븯뒗 寃껋쓣 솗씤븷 닔 엳뿀떎. T 꽭룷 遺꾩뿴珥됱쭊씤옄씤 ConA瑜 씠슜븳 T 꽭룷 利앹떇뒫쓣 痢≪젙븳 寃곌낵, 젙긽議곌뎔 4.62짹6.55 pg/mL怨 鍮꾧탳븯쓣 븣, 硫댁뿭뼲젣援곗뿉꽌 1.68짹1.56 pg/mL쑝濡 쁽븯寃 媛먯냼븯쑝굹 쑀쓽꽦 굹굹吏 븡븯떎. 硫댁뿭뼲젣援곌낵 鍮꾧탳븯쓣 븣, 諛쒗슚궗臾쇳깢쓣 닾뿬븳 援곗뿉꽌 紐⑤몢 냽룄 쓽議댁쟻쑝濡 利앷븯뒗 슚怨쇰 蹂댁쑝굹, 쑀쓽꽦 굹굹吏 븡븯떎(Fig. 4).

Fig. 3. Effects of fermented samultangextracton B cell proliferation changes in mouse with immunsuppression induced by cyclophosphamide. After MTT assay, it was measured at 570 nm using an ELISA Reader. Notes on group designation at the same as Fig. 2. Data are shown as mean 짹 SE (n=7). Significant difference from I group by Student's t-test: *P<0.05.

Fig. 4. Effects of fermented samultangextracton T cell proliferation changes in mouse with immunsuppression induced by cyclophosphamide. Notes on group designation at the same as Fig. 2. Data are shown as mean 짹 SE (n=7).

由쇳봽援 븘삎 寃궗

諛쒗슚궗臾쇳깢쓣 븿쑀븳 湲곕낯二 닾뿬뿉 쓽븳 硫댁뿭젰 媛쒖꽑 슚怨쇰 룊媛븯湲 쐞빐 鍮꾩옣꽭룷뿉꽌 由쇳봽援 븘삎 寃궗瑜 吏꾪뻾븯떎. B 꽭룷瑜 몴쟻 留덉빱 CD19, T 꽭룷瑜 蹂닿린 쐞븳 CD3, NK 꽭룷瑜 蹂닿린 쐞븳 CD56쓣 痢≪젙븯떎(Table 1). CD19瑜 痢≪젙븳 寃곌낵, 젙긽議곌뎔 1245.2짹571.5 ng/mL怨 鍮꾧탳븯쓣 븣, 硫댁뿭뼲젣援곗뿉꽌 785.2짹241.0 ng/mL濡 媛먯냼븯怨, 쑀쓽꽦 굹굹吏 븡븯떎. 硫댁뿭뼲젣援곌낵 鍮꾧탳븯쓣 븣, 떆뿕臾쇱쭏 닾뿬援곗뿉꽌 냽룄 쓽議댁쟻쑝濡 利앷븯쑝硫, BH援곗뿉꽌 996.4짹190.5 ng/mL濡 利앷븯쑝굹 쑀쓽븳 李⑥씠뒗 굹굹吏 븡븯떎. CD3쓣 痢≪젙븳 寃곌낵, 젙긽議곌뎔 570짹172.8 ng/mL 鍮꾧탳븯쓣 븣, 硫댁뿭뼲젣援곗뿉꽌 390.1짹22.2 ng/mL濡 媛먯냼븯쑝굹 쑀쓽꽦 굹굹吏 븡븯떎. 硫댁뿭뼲젣援곌낵 鍮꾧탳븯쓣 븣, 떆뿕臾쇱쭏 닾뿬援곗뿉꽌 냽룄 쓽議댁쟻쑝濡 利앷븯쑝硫, 듅엳 BH援곗뿉꽌 537.3짹115.7 ng/mL濡 쑀쓽븯寃 利앷(P<0.05)븯떎. CD56쓣 痢≪젙븳 寃곌낵 젙긽議곌뎔 34.3짹15.8 ng/mL怨 鍮꾧탳븯쓣 븣, 硫댁뿭뼲젣援곗뿉꽌 24.5짹6.8 ng/mL濡 媛먯냼븯쑝굹 쑀쓽븳 李⑥씠뒗 굹굹吏 븡븯떎. 硫댁뿭뼲젣援곌낵 떆뿕臾쇱쭏 닾뿬援곗쓣 鍮꾧탳븯쓣 븣, 냽룄 쓽議댁쟻쑝濡 利앷븯怨, 듅엳 BH援곗뿉꽌 36.3짹8.0 ng/mL濡 쑀쓽븯寃 利앷(P<0.05)븯떎.

Effects of fermented samultangextracton CD19, CD3, CD56 changes in mouse with immunsuppression induced by cyclophosphamide

Group CD3 CD19 CD56
NC 540.7짹70.5 1245.2짹233.3 34.3짹6.5
I 390.13짹9.07 785.2짹98.4 24.5짹2.8
PC 401.0짹18.0 794.3짹217.7 30.4짹2.3
BL 451.9짹62.3 864.8짹126.0 28.3짹2.0
BM 451.9짹62.3 751.2짹39.8 28.1짹0.7
BH 537.3짹47.2* 996.4짹77.8 36.3짹3.2*

Notes on group designation at the same as Fig. 2. Data are shown as mean 짹 SE (n=7). Significant difference from I group by Student's t-test: *P<0.05



硫댁뿭湲濡쒕텋由 寃궗

諛쒗슚궗臾쇳깢쓣 븿쑀븳 湲곕낯二 닾뿬뿉 쓽븳 硫댁뿭젰 媛쒖꽑 슚怨쇰 룊媛븯湲 쐞빐 룞臾쇱쓽 삁泥뿉꽌 硫댁뿭湲濡쒕텋由 寃궗瑜 吏꾪뻾븯떎(Table 2). IgA瑜 痢≪젙븳 寃곌낵, 젙긽議곌뎔 480짹131 ng/mL怨 鍮꾧탳븯쓣 븣, 硫댁뿭뼲젣援곗뿉꽌 48짹69 ng/mL濡 쁽븯寃 媛먯냼븯쑝硫 쑀쓽븳 寃곌낵瑜 굹궡뿀떎(P<0.05). 硫댁뿭뼲젣援곌낵 떆뿕臾쇱쭏 닾뿬援곗쓣 鍮꾧탳븯쓣 븣, BM援 372짹173 ng/mL, BH援 220짹212 ng/m濡 쑀쓽븯寃 利앷(P<0.05)븯쑝硫, 諛쒗슚궗臾쇳깢 二 닾뿬援 紐⑤몢 硫댁뿭뼲젣援곌낵 鍮꾧탳븯쓣 븣 20% 씠긽 利앷븯뒗 寃곌낵瑜 蹂댁떎(Keyt et al., 2020). IgM쓣 痢≪젙븳 寃곌낵, 젙긽議곌뎔 190짹69 ng/mL怨 쑀쓽븳 李⑥씠뒗 굹굹吏 븡븯떎. 硫댁뿭뼲젣援곌낵 鍮꾧탳븯쓣 븣, 떆뿕臾쇱쭏 닾뿬援곗뿉꽌 利앷븯吏留, 쑀쓽꽦 굹굹吏 븡븯떎. IgG瑜 痢≪젙븳 寃곌낵, 젙긽議곌뎔 1258짹442 ng/mL怨 鍮꾧탳븯쓣 븣, 硫댁뿭뼲젣援곗뿉꽌 646짹209 ng/mL濡 쁽븯寃 媛먯냼븯쑝硫, 鍮꾨줉 쑀쓽꽦 굹굹吏 븡븯쑝굹 硫댁뿭뼲젣젣瑜 닾뿬븳 紐⑤뱺 援곗뿉꽌 媛먯냼븯뒗 寃쏀뼢쓣 蹂댁떎. 諛섎㈃, 湲곕낯二쎌쓣 닾뿬븯쓣 븣 媛먯냼맂 IgG媛 利앷븯吏 븡븯쑝굹, 諛쒗슚궗臾쇳깢 二쎌쓣 닾뿬븳 紐⑤뱺 援곗뿉꽌 쑀쓽꽦 굹굹吏 븡븯吏留 利앷븯뒗 寃쏀뼢쓣 굹궡뿀떎.

Effects of fermented samultang extracton immunoglobulin A, immunoglobulin M, immunoglobulin G changes in mouse with immunsuppression induced by cyclophosphamide

Group IgA IgG IgM
NC 480.3짹53.5 1258.1짹180.6 189.7짹28.1
I 47.6짹28.2# 645.6짹85.2 176.6짹37.7#
PC 144짹90.7 490.8짹165.6 98.6짹56.3
BL 115.9짹44.5 713.8짹97.7 146.9짹58.4
BM 372.1짹70.6* 785.5짹161.7 166.5짹31.7
BH 220.1짹86.6* 788.2짹186.5 192.4짹42.5

Notes on group designation at the same as Fig. 2. Data are shown as mean 짹 SE (n=7). Significant difference from Normal group by Student's t-test: #P<0.05. Significant difference from I group by Student's t-test: *P<0.05



議곗쭅蹂묐━븰쟻 寃궗

Haematoxylin & Eosin (H&E) 뿼깋쓣 넻븯뿬 鍮꾩옣 諛 쓨꽑쓽 蹂묐━븰쟻 蹂솕瑜 愿李고븯떎. 젙긽議곌뎔쓽 鍮꾩옣議곗쭅 以묒떖 젙留(central vein, 씠븯 CA) 二쇰쑝濡 諛깆깋닔吏덉씠 怨좊Ⅴ寃 遺꾪룷릺뼱 엳쑝硫, 由쇳봽냼젅(lymph nodule, 씠븯 LN)씠 媛옣옄由 援ъ뿭뿉 몮윭떥뿬 엳뼱 쟻깋닔吏덇낵쓽 寃쎄퀎媛 紐낇솗븯寃 援щ텇맂 寃껋쑝濡 愿李곕릺뿀떎(Park et al., 2001). 諛깆깋 닔吏덉쓽 鍮꾩쑉쓣 痢≪젙븯湲 쐞빐 쁽誘멸꼍쓣 씠슜븯뿬 200諛곗쓽 鍮꾩쑉濡 珥ъ쁺 썑 image j program쓣 씠슜븯뿬 鍮꾩옣쓽 쟾泥 硫댁쟻 鍮 諛깆깋닔吏덉쓽 硫댁쟻쓣 痢≪젙븯뿬 鍮꾩쑉濡 怨꾩궛븯떎. 硫댁뿭뼲젣援곗뿉꽌 諛깆깋 닔吏덉쓽 鍮꾩쑉씠 쁽븯寃 媛먯냼븳 諛섎㈃ 硫댁뿭뼲젣援 19.4짹4.9% 鍮꾧탳븯쓣 븣, 떆뿕臾쇱쭏 닾뿬援곗뿉꽌 諛깆깋닔吏덉쓽 鍮꾩쑉씠 냽룄 쓽議댁쟻쑝濡 利앷븯쑝硫 듅엳 BM 39.8짹7.4%, BH 44.0짹9.6%뿉꽌 쑀쓽븯寃 利앷븯뿬 議곌뎔뿉꽌 愿李곕릺뜕 諛깆깋닔吏덉쓽 遺뺢눼뒗 嫄곗쓽 솗씤릺吏 븡븯떎(P<0.05) (Fig 5, 6). 떎쓬쑝濡, 쓨꽑뿉꽌 닔吏덉씠 李⑥븯뒗 硫댁쟻 鍮꾩쑉쓣 痢≪젙븯湲 쐞빐 쁽誘멸꼍쓣 씠슜븯뿬 200諛곗쓽 鍮꾩쑉濡 珥ъ쁺 썑 image j program쓣 씠슜븯뿬 쓨꽑쓽 쟾泥 硫댁쟻 鍮 닔吏덉쓽 硫댁쟻쓣 痢≪젙븯뿬 鍮꾩쑉濡 怨꾩궛븯떎. 젙긽議곌뎔뿉꽌뒗 쐞 媛숈 議곗쭅蹂묐━븰쟻 냼寃ъ 굹굹吏 븡뒗 諛섎㈃, 硫댁뿭뼲젣援곗뿉꽌 뵾吏 궡 TBM쓽 利앷, 닔吏덉씠 李⑥븯뒗 硫댁쟻쓽 鍮꾩쑉씠 쑀쓽븯寃 媛먯냼(P<0.05)븯쑝硫, 닔吏덇낵 뵾吏덉쓽 寃쎄퀎媛 遺덈챸솗븳 寃곌낵瑜 蹂댁떎. 삉븳 硫댁뿭뼲젣援곌낵 鍮꾧탳븯쓣 븣, 諛쒗슚궗臾쇳깢 二 닾뿬援곗뿉꽌 닔吏덉씠 李⑥븯뒗 鍮꾩쑉씠 利앷븯쑝硫, 쑀쓽븳 李⑥씠뒗 굹굹吏 븡븯쑝굹(Fig. 7, 8) 硫댁뿭뼲젣援곗뿉꽌 蹂댁뜕 쑀쓽븳 냼寃щ뱾씠 냽룄 쓽議댁쟻쑝濡 媛쒖꽑릺뒗 슚怨쇰 愿李고븷 닔 엳뿀떎.

Fig. 5. Effect of fermented samultang extracton spleen histological changes by H&E staining in cyclophosphamide induced immunsuppression in mouse (횞100). Notes on group designation at the same as Fig. 2.

Fig. 6. Effects of fermented samultang extracton white pulp rate changes in mouse with immunsuppression induced by cyclophosphamide. Notes on group designation at the same as Fig. 1. Data are shown as mean 짹 SE (n=7).

Fig. 7. Effect of fermented samultang extract on thymus histological changes by H&E staining in cyclophosphamide induced immunsuppression in mouse (횞100). Notes on group designation at the same as Fig. 2.

Fig. 8. Effects of fermented samultang extract on Thymus meddulla rate changes in mouse with immunsuppression induced by cyclophosphamide. Notes on group designation at the same as Fig. 2. Data are shown as mean 짹 SE (n=7). Significant difference from normal group by Student's t-test: #P<0.05.
怨 李

蹂 뿰援щ뒗 諛쒗슚궗臾쇳깢씠 븿쑀맂 二쎌쓽 硫댁뿭솢꽦利앹쭊 슚怨쇰 솗씤븯湲 쐞빐 CPA濡 쑀諛쒗븳 硫댁뿭뼲젣 룞臾쇰え뜽쓣 씠슜븯뿬 뿰援ы븯떎. 씪諛섏쟻쑝濡 깮泥 궡 硫댁뿭湲곕뒫 痢≪젙 湲곗쑝濡 씤吏릺뒗 쓨꽑 誘 鍮꾩옣 吏닔뒗 떇씠 꽦遺꾩뿉 쓽빐 쑀쓽쟻씤 쁺뼢쓣 諛쏅뒗떎怨 븣젮졇 엳떎. 떇씠濡 씤븳 泥대궡 痢≪젙移 李⑥씠뒗 遺遺꾩쟻쑝濡 由쇳봽援 깮꽦 뒫젰쓽 李⑥씠瑜 珥덈옒븯怨, 씠寃껋 듅젙 吏덊솚 媛먯뿼 떆 뜑슧 겙 쁺뼢쓣 諛쏅뒗 寃껋쑝濡 빐꽍릺怨 엳떎(Cho et al., 2013). 깮泥 궡 硫댁뿭湲곕뒫 痢≪젙 湲곗쑝濡 씤吏릺뒗 쓨꽑 諛 鍮꾩옣쓽 臾닿쾶瑜 痢≪젙븯쓣 븣, Kim 벑쓽 蹂닿퀬 媛숈씠 CPA 닾뿬援곗쓽 쓨꽑以묐웾 媛먯냼븯怨, 鍮꾩옣쓽 以묐웾 硫댁뿭젰 븯뿉 뵲瑜 蹂댄샇옉슜쑝濡 利앷븯뒗 寃껋쓣 愿李고븯떎(Kim, 2020). 諛섎㈃, 諛쒗슚궗臾쇳깢 二 以묐냽룄瑜 닾뿬븳 援곗뿉꽌 鍮꾩옣 臾닿쾶媛 媛옣 겕寃 媛먯냼븯쑝硫, 씠뒗 씪諛 嫄닿컯二쎌쓣 꽠痍⑦븳 뼇꽦議곌뎔 蹂대떎 겕寃 媛먯냼븯뒗 寃껋쓣 蹂 닔 엳뿀떎. 씠윭븳 二쇱슂 옣湲 臾닿쾶쓽 蹂솕뒗 諛쒗슚궗臾쇳깢씠 硫댁뿭뼲젣젣씤 CPA濡 씤빐 쑀諛쒕맂 硫댁뿭젰 븯 諛섏쓳쓣 뼲젣븯뿬 硫댁뿭利앹쭊 옉슜뿉 쁺뼢쓣 겮移 寃껋쑝濡 궗猷뚮맂떎(Kang, 2014). 鍮꾩옣 궡뿉 議댁옱븯뒗 T, B 由쇳봽援щ 痢≪젙븯쓣 븣, CPA 닾뿬濡 씤빐 媛먯냼릺뒗 寃껋쓣 솗씤븯쑝硫, 諛쒗슚궗臾쇳깢 二쎌쓣 닾뿬븯쓣 븣 넻怨꾪븰쟻쑝濡 쑀쓽븯吏 븡 遺遺꾨룄 엳쑝굹 냽룄 쓽議댁쟻쑝濡 利앷븯뒗 寃쏀뼢쓣 蹂댁떎. 떎쓬쑝濡 諛쒗슚궗臾쇳깢 二쎌뿉 쓽븳 硫댁뿭젰 媛쒖꽑 슚怨쇰 룊媛븯湲 쐞빐 룞臾쇱쓽 鍮꾩옣꽭룷뿉꽌 B, T 꽭룷 利앹떇뒫쓣 痢≪젙븯떎. 鍮꾩옣 씠李 由쇳봽議곗쭅쑝濡 옉슜븯뿬 삁븸 냽 빆썝쓣 젣嫄고븯怨 硫댁뿭꽭룷瑜 솢꽦솕떆耳 쇅遺 빆썝뿉 빐 쟻쓳 硫댁뿭諛섏쓳쓣 떆옉븯뒗 二쇱슂 옣湲곗씠떎. CPA 궗泥대뱾 鍮꾩옣꽭룷쓽 DNA瑜 akylation 떆耳, T, B 由쇳봽援ъ뿉 쓽븳 硫댁뿭諛섏쓳쓣 뼲젣떆궓떎怨 븣젮졇 엳떎(Choi et al., 2019). CPA濡 硫댁뿭뼲젣瑜 쑀룄븯쓣 븣, T, B 由쇳봽援ъ쓽 쁽븳 媛먯냼媛 굹궗쑝硫, 씠뒗 CPA 닾뿬뿉 쓽븳 T B 由쇳봽援ъ쓽 利앹떇뒫쓣 媛먯냼떆궓 寃껋 由쇳봽援ъ쓽 mitogen 쑀룄꽦 利앹떇諛섏쓳쓣 솗씤븳 寃곌낵 mitogen뿉 諛섏쓳븳 由쇳봽援ш 궙寃 굹궗떎뒗 꽑뻾뿰援 寃곌낵 씪移섑븯떎(Shin et al., 1996). 諛섎㈃, 諛쒗슚궗臾쇳깢 二쎌쓣 닾뿬븳 紐⑤뱺 援곗뿉꽌 냽룄 쓽議댁쟻쑝濡 利앷븯뒗 슚怨쇰 蹂댁쑝硫 듅엳, 諛쒗슚궗臾쇳깢 二 以, 怨좊냽룄 닾뿬援곗뿉꽌뒗 湲곕낯二쎌쓣 닾뿬븳 뼇꽦議곌뎔怨 鍮꾧탳븯쓣 븣, 빟 2諛 씠긽 利앷븯뒗 寃곌낵瑜 굹궡뿀떎. 諛쒗슚궗臾쇳깢 二 닾뿬援곗쓽 T 꽭룷 B 꽭룷 利앹떇 鍮꾩옣 궡 硫댁뿭꽭룷 솢꽦쑝濡 빐꽍릺怨, 諛쒗슚궗臾쇳깢씠 鍮꾩옣꽭룷쓽 利앹떇뿉룄 슚怨쇨 엳쓬쓣 利앸챸븳 Lee 벑쓽 뿰援ш껐怨쇱 씪移섑븯떎(Lee et al., 2009). 씠 쇅뿉룄 諛쒗슚궗臾쇳깢쓣 援ъ꽦븯뒗 꽦遺꾩씤 諛깆옉빟 異붿텧臾쇱쓽 議곕떎떦遺꾪쉷 臾쇱쭏씠 T 꽭룷뿉 쁺뼢쓣 二쇱뼱 cytokine쓣 쑀諛쒗븯怨 씠뱾씠 떇꽭룷 B 꽭룷瑜 솢꽦솕떆耳 硫댁뿭議곗젅臾쇱쭏濡 궗슜맂떎뒗 Park 벑쓽 뿰援ъ뿉 뵲씪, T, B 꽭룷 솢꽦솕뿉 湲곗뿬뻽쓣 寃껋쑝濡 궗猷뚮맂떎(Park et al., 2001). 삉븳, 諛쒗슚궗臾쇳깢 二쎌쓣 닾뿬븯뿬 利앷맂 p38 Mitogen-activatedprotein kinase (MAPK)쓽 諛쒗쁽씠 媛먯냼릺뿀떎뒗 꽑뻾뿰援 寃곌낵濡 鍮꾩텛뼱 蹂댁븯쓣 븣, 諛쒗슚궗臾쇳깢 二쎌씠 mitogen쓽 議곗젅뿉 愿뿬븯뿬 굹궃 寃곌낵濡 蹂댁뿬吏꾨떎(Kim et al., 2014). 鍮꾩옣꽭룷 궡 由쇳봽援 븘삎쓽 遺꾪룷 蹂솕瑜 솗씤븯湲 쐞빐, B, T 由쇳봽援ъ NK 꽭룷瑜 CD19, CD3, CD56 marker瑜 씠슜빐 痢≪젙븳 寃곌낵 젙긽議곌뎔뿉 鍮꾪빐 紐⑤뱺 硫댁뿭뼲젣쑀룄援곗뿉꽌 媛먯냼븯쑝硫, 씠뒗 R. Huang 벑쓽 뿰援ъ뿉꽌 CPA 닾뿬濡 씤빐 CD19, CD3쓽 닔移섍 媛먯냼븳 寃껉낵 룞씪븳 寃곌낵瑜 굹궡뿀떎(Huang et al., 2013). 諛섎㈃, 硫댁뿭뼲젣援곌낵 諛쒗슚궗臾쇳깢 닾뿬援곗쓣 鍮꾧탳븯쓣 븣 諛쒗슚궗臾쇳깢 二 닾뿬援곗뿉꽌 냽룄 쓽議댁쟻쑝濡 利앷븯떎. 듅엳, CD3怨 CD56뿉꽌 BH援곗씠 쑀쓽븯寃 利앷븯떎. 肉먮쭔 븘땲씪, 諛쒗슚궗臾쇳깢 二 怨좊냽룄 닾뿬援곗뿉꽌 湲곕낯二쎌쓣 닾뿬븳 뼇꽦議곌뎔蹂대떎 CD19, CD3, CD56쓽 諛쒗쁽씠 利앷븯쑝硫, 젙긽議곌뎔怨 쑀궗븯嫄곕굹 빟媛 넂寃 痢≪젙맂 寃껋쑝濡 遺꾩꽍릺뿀떎. 삉븳, 룞臾쇱쓽 삁泥뿉꽌 硫댁뿭湲濡쒕텋由곗쓣 IgG, IgA, IgM쓽 諛쒗쁽쓣 痢≪젙븯떎. IgA뒗 諛붿씠윭뒪媛 샇씉湲 몴뵾꽭룷뿉 遺숈 떎쓬 몴뵾痢듭쓣 넻怨쇳븯뿬 꽭룷 궡濡 移⑥엯븳 뮘 踰덉떇븯寃 릺뒗 怨쇱젙씠 吏꾪뻾릺뒗 寃껋쓣 留됱븘二쇰뒗 뿭븷쓣 븳떎怨 븣젮졇 엳쑝硫(Patr챠cia and Woof, 2019), IgG뒗 삁泥뿉꽌 媛옣 뭾遺븳 빆泥대줈꽌 硫댁뿭湲濡쒕텋由곗쓽 80%瑜 李⑥븯怨 엳떎(Papadea and Check, 1989)怨 븣젮졇 엳떎. 痢≪젙寃곌낵, IgA IgG뿉꽌 議곌뎔뿉 鍮꾪빐 硫댁뿭뼲젣援곗뿉꽌 쑀쓽븯寃 媛먯냼븯怨, IgA쓽 寃쎌슦 硫댁뿭뼲젣援곗뿉 鍮꾪빐 BM援곌낵 BH援곗뿉꽌 쑀쓽븯寃 利앷븯쑝硫, 씠뒗 湲곕낯二쎌쓣 닾뿬븳 뼇꽦議곌뎔蹂대떎 2諛 媛源뚯씠 利앷븯뒗 寃쏀뼢쓣 굹궡뿀떎. 留덉留됱쑝濡, 鍮꾩옣怨 쓨꽑쓽 蹂묐━븰쟻 蹂솕瑜 愿李고븯쓣 븣, 鍮꾩옣議곗쭅 CA 二쇰쑝濡 諛깆깋닔吏덉씠 怨좊Ⅴ寃 遺꾪룷릺뼱 엳怨, LN씠 媛옣옄由ъ뿉 몮윭떥뿬 엳뼱 쟻깋닔吏덇낵 寃쎄퀎媛 紐낇솗븯寃 援щ텇릺뿀떎 硫댁뿭뼲젣援곗 CPA濡 씤븳 諛깆깋닔吏 넀긽쑝濡 CA 쟻깋닔吏덇낵쓽 寃쎄퀎媛 遺덈챸솗븯뒗뜲, 씪諛섏쟻쑝濡 CPA瑜 泥섎━븯寃 릺硫 鍮꾩옣쓽 諛깆깋닔吏덉씠 넀긽릺뼱 以묒떖젙留, 쟻깋닔吏덇낵쓽 寃쎄퀎媛 遺덈챸솗븯떎怨 蹂닿퀬맂 寃곌낵 媛숈 냼寃ъ쓣 蹂댁떎(Chung et al., 1978). 諛섎㈃, 諛쒗슚궗臾쇳깢 二 닾뿬援곗 諛깆깋닔吏덉쓽 鍮꾩쑉씠 넻怨꾪븰쟻쑝濡 쑀쓽븯吏 븡 遺遺꾨룄 엳쑝굹 냽룄 쓽議댁쟻쑝濡 利앷븯뒗 寃쏀뼢쓣 蹂댁쑝硫, CPA瑜 떒룆 닾뿬븳 議곌뎔뿉꽌 愿李곕맂 諛깆깋닔吏덉쓽 遺뺢눼뒗 愿李곕릺吏 븡븯떎. 씠뒗 諛쒗슚궗臾쇳깢쓽 援ъ꽦臾쇱쭏씤 떦洹 異붿텧臾쇱쓣 닾뿬븯쓣 , 鍮꾩옣 議곗쭅쓽 닔吏덇컙쓽 寃쎄퀎媛 紐낇솗븯寃 援щ텇릺硫 議곗쭅 궡 媛옣옄由 援ъ뿭씠 꽑紐낇븯寃 愿李곕릺硫 쟻깋닔吏덉쓽 꽭룷 쓳異 쁽긽씠 샇쟾맂떎뒗 Seo Kim쓽 蹂닿퀬 씪移섑븯뒗 寃쏀뼢쓣 굹궡뿀떎(Seo and Kim, 2022). 삉 떎瑜 二쇱슂 硫댁뿭 옣湲 以 븯굹씤 쓨꽑쓽 뵾吏덉 떆뿕臾쇱쭏뿉 쓽븳 吏곸젒쟻 쁺뼢 삉뒗 뒪듃젅뒪뿉 쓽빐 꽭룷 닔 媛먯냼 蹂묐씠 굹궇 닔 엳쑝硫, 由쇳봽援 옄硫몄궗 利앷, 뵾吏 궡 Tingible Body Macrophages (TBM)쓽 利앷, 뵾吏덇낵 닔吏 궗씠 寃쎄퀎 媛먯냼瑜 룞諛섑븯寃 맂떎. 쓨꽑쓽 寃쎌슦 젙긽議곌뎔뿉꽌뒗 由쇳봽援 옄硫, TBM 利앷, 뵾吏덇낵 닔吏덉쓽 寃쎄퀎 媛먯냼 벑怨 媛숈 議곗쭅蹂묐━븰쟻 냼寃ъ 蹂댁씠吏 븡븯怨, 硫댁뿭뼲젣援곗 뵾吏 궡 TBM 利앷, 닔吏덈㈃쟻 鍮꾩쑉 媛먯냼, 닔吏덇낵 뵾吏덉쓽 寃쎄퀎媛 遺덈텇紐낇븳 냼寃ъ쓣 蹂댁떎. 씠윭븳 썝씤 硫댁뿭뼲젣젣濡 씤븳 쓨꽑꽭룷쓽 꽭룷궗硫몄쓣 넻븳 쓨꽑쐞異뺤쑝濡 궗猷뚮맂떎(Lee, 2014). 諛섎㈃ 硫댁뿭뼲젣援곗뿉 鍮꾪빐 諛쒗슚궗臾쇳깢 二 닾뿬援곗뿉꽌 닔吏덈㈃쟻 鍮꾩쑉씠 利앷븯吏留, 쑀쓽븳 李⑥씠뒗 뾾뿀떎. 蹂 뿰援щ 넻빐 옄泥 媛쒕컻븳 諛쒗슚궗臾쇳깢 二쎌씠 硫댁뿭솢꽦利앹쭊뿉 슚怨쇨 엳쓬쓣 솗씤븯怨, 怨좊졊솕濡 씤븳 끂씤쓽 옉슫룞 븯濡 諛쒖깮븯뒗 쁺뼇遺덇퇏삎 諛 硫댁뿭젰 븯뿉 룄쓣 以 寃껋쑝濡 깮媛곷맂떎. 뵲씪꽌, 諛쒗슚궗臾쇳깢 二쎌 硫댁뿭利앹쭊怨 愿젴맂 湲곕뒫꽦 냼옱濡 솢슜쓣 湲곕븷 닔 엳怨, 떇뭹媛쒕컻쓽 냼옱濡쒖뜥 솢슜븷 寃쎌슦 怨좊졊솕 떆쓽 궗쉶쟻 鍮꾩슜 젅媛먭낵 궣쓽 吏 媛쒖꽑뿉룄 留롮 湲곗뿬瑜 븷 닔 엳쓣 寃껋쑝濡 湲곕맂떎.

ACKNOWLEDGEMENT

None.

CONFLICT OF INTEREST

No potential conflict of interest relevant to this article was reported.

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