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Angelica gigas Nakai Attenuates Mast Cell-mediated Inflammation via Suppression of Caspase-1 Activation
Biomed Sci Letters 2024;30:238-247
Published online December 31, 2024;  https://doi.org/10.15616/BSL.2024.30.4.238
© 2024 The Korean Society For Biomedical Laboratory Sciences.

Su Jin Kim†,*

Department of Biotechnology and Convergence, Daegu Haany University, Gyeongsan 38578, Korea
Correspondence to: †Su Jin Kim
Department of Biotechnology and Convergence, Daegu Haany University, 285-10 Eobongji-gil, Gyeongsan 38578, Korea
Tel: +82-53-819-1389
E-mail: ksj1009@dhu.ac.kr
ORCID: https://orcid.org/0000-0003-2389-0336

*Professor.
Received September 4, 2024; Revised December 2, 2024; Accepted December 6, 2024.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
 Abstract
Objectives: Natural products are crucial sources for drug development due to their diverse biological properties. Angelica gigas Nakai (AG) has been traditionally used as an effective herbal medicine for diseases treatment, but its accurate anti-inflammatory mechanism is not well understood. The study aimed to investigate the mechanisms of action of AG on mast cell-mediated inflammatory responses.
Methods: The antioxidant activity of AG was assessed using 2,2-diphenyl-1-picrylhydrazy (DPPH) scavenging ability and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) cation scavenging. To explore AG’s pharmacological mechanism on inflammation, we evaluated its impact on histamine release and interleukin (IL)-6, IL-8, and tumor necrosis factor (TNF)-α production in activated human mast cells-1 (HMC-1). Additionally, the inhibitory effects of AG on activation of nuclear factor-κB (NF-κB) and caspase-1 were determined.
Results: AG demonstrated strong DPPH and ABTS+ radical scavenging activity, and significantly suppressed histamine, IL-8, IL-6, and TNF-α production. Moreover, AG effectively ameliorated the activation of NF-κB and caspase-1 in activated-HMC-1.
Conclusion: These finding suggest that AG has potential for inflammation therapy.
Keywords : Angelica gigas Nakai, Inflammation, Mast cells, Histamine, NF-kappa B, Caspase-1