Search for


TEXT SIZE

search for




CrossRef (0)
Time-course Analysis of Bone Loss Induced by Bilateral Ovariectomy in BDF-1 Hybrid Mice
Biomed Sci Letters 2024;30:248-254
Published online December 31, 2024;  https://doi.org/10.15616/BSL.2024.30.4.248
© 2024 The Korean Society For Biomedical Laboratory Sciences.

Dahyeon Yoo1* and Jaewang Lee2,†,**

1Department of Biomedical Laboratory Science, Eulji University, Seongnam 13135, Korea
2Department of Senior Healthcare, Graduate School of Eulji University, Seongnam 13135, Korea
Correspondence to: †Jaewang Lee
Department of Biomedical Laboratory Science, Eulji University, 553 Sanseong-daero, Sujeong-gu, Seongnam 13135, Korea
Tel: +82-31-740-7144
E-mail: wangjaes@gmail.com
ORCID: https://orcid.org/0000-0001-6801-7149

*Graduate student, ** Professor.
Received October 16, 2024; Revised December 9, 2024; Accepted December 9, 2024.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
 Abstract
Objectives: Osteoporosis is a major health concern in postmenopausal women. The aim of this study was to investigate the time-course bone loss phenomenon caused by bilateral ovariectomy (biOVX) through bioimaging, biochemical, and histological analyses.
Methods: In this study, 8-week-old BDF-1 hybrid female mice were subjected to biOVX to induce bone loss. Following biOVX, the mice were randomly assigned to five groups (sham control without biOVX, and groups sacrificed at 4, 8, and 12 weeks post-biOVX). The mice were sacrificed to collect dual-energy X-ray absorptiometry images, blood serum, and bone samples for further analysis at 4, 8, and 12 weeks post-biOVX.
Results: Four weeks after biOVX, all osteoporosis-related biomarkers (serum Ca²+, Mg²+, bone mineral content, bone mineral density [BMD], and bone volume [BV] in the femur) were significantly decreased compared with sham controls. Bilateral OVX continued to show reduced serum Ca²+, BMD, and BV at 8 weeks post-surgery. Histomorphological changes in the femoral bone were also observed at 4 weeks and persisted until 12 weeks post-biOVX.
Conclusion: This study confirmed the time-course bone loss caused by biOVX through X-ray imaging, biochemical, and histological analyses. Based on these findings, we suggest that biOVX is a robust model for studying osteoporosis. However, further studies are still required.
Keywords : Ovariectomy, Bone loss, Dual-energy X-ray absorptiometry, Biochemical analysis, Histology